長鏈非編碼RNA AK125512在肝癌組織中的表達及其臨床意義

蔡杰，林孔英，袁聲賢，陶其飛，徐慶國，余建，黃位龍，汪珍光，楊遠，林川，周偉平（第二軍醫大學附屬東方肝膽外科醫院　肝外三科，上海　200433）

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長鏈非編碼RNA AK125512在肝癌組織中的表達及其臨床意義

蔡杰，林孔英，袁聲賢，陶其飛，徐慶國，余建，黃位龍，汪珍光，楊遠，林川，周偉平
（第二軍醫大學附屬東方肝膽外科醫院肝外三科，上海200433）

[摘 要]目的 檢測長鏈非編碼RNA AK125512在肝癌、癌旁肝組織中的表達情況，探討其與臨床病理指標的關系及作為預測肝癌患者術后預后標記物的潛在價值。方法 從本課題組已完成表達譜芯片（GSE54238）中篩選明顯差異表達lncRNA-AK125512，采用實時熒光定量PCR（qRT-PCR）法檢測lncRNAAK125512在肝癌、癌旁肝組織中的表達；x2檢驗分析肝癌組織中lncRNA-AK125512表達量與臨床病理指標相關性；應用Log-rank檢驗和COX回歸模型分析肝癌組織中lncRNA-AK125512表達量與肝癌患者術后預后的關系。結果 lncRNA-AK125512在肝癌組織中較癌旁肝組織明顯低表達（P＜0.0001）；肝癌組織中lncRNA-AK125512的表達水平與Edmondson分級（P=0.022）、腫瘤包膜完整與否（P=0.005）、血AFP（P=0.018）明顯相關；肝癌組織中lncRNA-AK125512相對低表達患者肝癌切除術后無瘤生存期（P=0.0041）及總體生存期（P=0.0008）均顯著縮短。肝癌組織中lncRNA-AK125512相對低表達是影響肝癌患者術后無瘤生存期（P= 0.037）、總體生存期（P=0.003）的一個獨立危險因素。結論 lncRNA-AK125512有望成為預測肝癌患者術后預后的一個新的潛在標記物。

[關鍵詞]長鏈非編碼RNA；癌，肝細胞；實時熒光定量PCR；預后

Expression of long non-coding RNA AK125512 in hepatocellular carcinoma and its clinical significance

CAI Jie,LIN Kong-ying,YUAN Sheng-xian,TAO Qi-fei,XU Qing-guo,YU Jian,HUANG Wei-long,WANG Zhen-guang,YANG Yuan,LIN Chuan,ZHOU Wei-ping.The Third Department of Hepatic Surgery,Eastern Hepatobiliary Hospital Affiliated to Second Military Medical University,Shanghai 200433,China

Abstract objectiveTo investigate the expression of the long non-coding RNA AK125512 in hepatocellular carcinoma(HCC)and its clinical significance.MethodsFrom the lncRNA microarray(GSE54238)which we completed before,lncRNA-AK125512 was selected because of its obvious expression difference.The realtime fluorescence quantitative PCR(qRT-PCR)was performed to detect the expression difference of lncRNAAK125512 between HCC and its adjacent normal tissue.Then,the correlation between the expression of lncRNA-AK125512 in HCC and the clinicopathological characteristics was analyzed by Chi-square test.What’s more,the potential of lncRNA-AK125512 in predicting the prognosis of patient with HCC after operation was assessed by Log-rank test and Cox’s proportional hazards regression model.ResultsThe expression of lncRNAAK125512 in HCC was markedly lower than that in its adjacent normal tissue(P＜0.0001).Moreover,the expression of lncRNA-AK125512 in HCC was significantly correlated with Edmondson classification(P=0.022),complete tumor capsules(P=0.005),alpha fetoprotein(AFP)serum levels(P=0.018).The patients with lower expression of lncRNA-AK125512 in HCC had a statistically shorter tumor-free survival time and overall survival time(P=0.0041,0.0008).What’s more,the lower expression of lncRNA-AK125512 in HCC was an independent risk factor for the tumor-free survival time(P=0.037)and overall survival time(P=0.003)of patients with HCC after curative hepatectomy.ConclusionlncRNA-AK125512 may be a new prognostic biomarker for hepatocel-lular carcinoma after curative hepatectomy.

Key words long non-coding RNA; hepatocellular carcinoma; real-time fluorescence quantitative PCR(qRTPCR); prognosis

肝癌是世界范圍內的高發惡性腫瘤之一，特別是在東亞、東南亞國家，而每年約一半的新發和死亡病例是發生在中國[1]。隨著現代醫療技術的進步，肝癌的診療手段已漸趨成熟，但是其術后5年的生存率仍只有15%～40%[2-3]，且每年的發病率與病死率基本持平?！?br>