PRDX1在人胰腺導管腺癌中的表達及其與病理指標的關系

張小薄，石剛，譚曉冬，楊一帆，王懷濤

(1.中國醫科大學附屬盛京醫院普通外科，遼寧沈陽110004；2.遼寧省腫瘤醫院大腸外科，遼寧沈陽110042)

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PRDX1在人胰腺導管腺癌中的表達及其與病理指標的關系

張小薄1，石剛2，譚曉冬1，楊一帆1，王懷濤1

(1.中國醫科大學附屬盛京醫院普通外科，遼寧沈陽110004；2.遼寧省腫瘤醫院大腸外科，遼寧沈陽110042)

[摘 要]目的 探討過氧化還原蛋白1(peroxiredoxin 1，PRDX1)在胰腺導管腺癌(pancreatic ductal adenocarcinoma，PDAC)中的表達及與臨床病理學參數的關系并評價其判斷預后的價值。方法 采用免疫組織化學法分析117例PDAC組織石蠟切片及41例癌旁正常胰腺組織石蠟切片中的PRDX1表達，對PRDX1表達水平與臨床病理指標進行相關性分析，進行生存分析。采用Western blotting和實時定量PCR(qRT-PCR)檢測9對配對新鮮PDAC組織及癌旁正常胰腺組織中PRDX1蛋白質和mRNA水平的表達。結果 免疫組化顯示PRDX1在PDAC中表達陽性率高于癌旁正常胰腺組織，差異有統計學意義(x2=9.761，P＜0.01)；新鮮PDAC中PRDX1蛋白及mRNA表達明顯高于配對的癌旁正常胰腺組織(t=9.187，P＜0.01；t=7.829，P＜0.011)；PRDX1表達與腫瘤直徑、腫瘤TNM分期、淋巴結轉移、病理分級有關(x2=8.876，P＜0.01；x2=4.342，P＜0.05；x2=9.273，P＜0.01；x2=6.943，P＜0.01)；PRDX1與術后復發率相關(x2=7.326，P＜0.01)，PRDX1表達與生存期呈負相關(r=-0.845，P＜0.05)。結論 PRDX1表達與PDAC的惡性程度有關，可作為判斷PDAC預后的預測因素。

[關鍵詞]胰腺導管腺癌；過氧化還原蛋白1(PRDX1)；生存分析

Expression and pathological signi■cance of peroxiredoxin 1 in human pancreatic ductal adenocarcinoma ZHANG Xiao-bao1, SHI Gang2, TAN Xiao-dong1, YANG Yi-fan1, WANG Huai-tao1.1Department of General Surgery, Shengjing Hospital Af■liated to China Medical University, Shenyang 110001, China;2Department of Colorectal Surgery, Liaoning Cancer Hospital, Shenyang 110042, China

Abstract ObjectiveTo explore the clinicopathological significance of PRDX1 expression in pancreatic ductal adenocarcinoma (PDAC) and evaluate its prognostic value.MethodsImmunohistochemistry was used to detect the expression of PRDX1 in 117 paraf■n-embedded PDAC specimens and 41 adjacent non-cancerous pancreatic tissues, the correlation between PRDX1 expression and clincopathological characteristics, survival and multifactor analysis were carried out.Western blotting and quantitative real-time polymerase chain reaction (qRTPCR) were used to detect the protein and mRNA expression in 9 paired fresh PDAC specimens and adjuvant noncancerous pancreatic tissues.ResultsPRDX1 was signi■cantly over-expressed in PDAC than that in adjacent non-cancerous pancreatic tissues (x2=9.761, P＜0.01).Western blotting and qRT-PCR showed that PRDX1 expression at protein and mRNA level were higher in PDAC tissues than those in adjacent non-cancerous pancreatic tissues (t=9.187, P＜0.01； t=7.829, P＜0.01).Tumor size, TNM stage, lymph node metastasis and pathological grade were correlated with the expression of PRDX1 (x2=8.876, P＜0.01； x2=4.342, P＜0.05； x2=9.273, P＜0.01； x2=6.943, P＜0.01).Post-operation recurrence rate was correlated with PRDX1 expression (x2=7.326, P＜0.01).The PRDX1 expression was negatively correlated with survival time (r=-0.845, P＜0.05).ConclusionPRDX1 expression is correlated with the malignant degree of PDAC and which may be used to predict the prognosis of PDAC.

Key words pancreatic ductal adenocarcinoma； peroxiredoxin 1； survival analysis

胰腺癌在發病早期即發生轉移，是復發率及死亡率最高的消化系惡性腫瘤之一，其中胰腺導管腺癌(pancreatic ductal adenocarcinoma，PDAC)占胰腺癌病理分型的80%以上[1-2]。過氧化還原蛋白(peroxiredoxins，PRDXs)是一類抗氧化酶，對細胞氧化活性具有調控作用，從而影響著細胞的分化、增殖及凋亡等生物學功能[3]。……