門靜脈輸注胰島至大鼠肝臟去細胞支架的結果考察

鄭祥韜，傅紅興，邱凱燕，何云強，張偉，高歌，王麗芳(.溫州醫科大學附屬第一醫院血管外科，浙江溫州5000；.溫州醫科大學藥學院，浙江溫州505；.寧波大學醫學院附屬醫院藥劑科，浙江寧波5000)

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門靜脈輸注胰島至大鼠肝臟去細胞支架的結果考察

鄭祥韜1，傅紅興2，邱凱燕2，何云強2，張偉3，高歌2，王麗芳2
(1.溫州醫科大學附屬第一醫院血管外科，浙江溫州325000；2.溫州醫科大學藥學院，浙江溫州325035；3.寧波大學醫學院附屬醫院藥劑科，浙江寧波315000)

[摘 要]目的 考察經門靜脈移植后的胰島細胞團在肝臟血管中停留的位置及栓塞部位細胞團的相關情況。方法 取成年SD大鼠，從門靜脈在體灌注去污劑十二烷基硫酸鈉溶液使肝臟去細胞化，再輸注經二苯基硫卡巴腙染色后的大鼠胰島，在體視顯微鏡和倒置顯微鏡下觀察去細胞后的肝臟內部的血管和胰島在肝臟內的栓塞情況。結果 大鼠肝臟在體去細胞后，可得到完全透明、清晰的肝臟支架，肝內血管分支豐富，經灌注(800±250)IEQ胰島細胞團后，胰島主要栓塞在大鼠肝臟邊緣血管末梢，有時一個血管末梢栓塞有多個細胞團。結論 本方法模擬大鼠門靜脈內胰島移植，呈現了胰島在肝臟內血管的駐留情況，有利于進一步研究改進胰島在肝內移植后的存活和功能發揮。

[關鍵詞]大鼠；肝去細胞支架；門靜脈；胰島移植

The investigation of islet infused into the rat decellularized liver bioscaffold in the body through portal vein

ZHENG Xiang-tao1, FU Hong-xing2, QIU Kai-yan2, HE Yun-qiang2, ZHANG Wei3, GAO Ge2, WANG Li-fang2.1Department of General Surgery, The First Af■liated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, China;2School of Pharmacy, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China;3Department of Pharmacy, Af■liated Hospital ofMedical School, Ningbo University, Ningbo, Zhejiang 315000, China

Abstract ObjectiveTo investigate the position and the circumstances of the islets transplanted in liver blood vessels by islets infusion through portal vein of rat.MethodsSD rats were chosen in this experiment, detergent sodium dodecyl sulfate was perfused into the liver from the portal vein to decellular the cells in the body.Then the rat islets stained by two diphenylthiocarbazone was also infused into the portal vein.The positions embolized by islets in the liver blood vessels were investigated from look inside the decellularized liver bioscaffold under stereomicroscope and inverted microscope.ResultsA completely transparent, clear liver bioscaffold was available after decellularization, which were rich in intrahepatic vascular branches in the body.After the infusion of 800±250 IEQ, the majority of rat islets were embolized in the microvascular in the peripheral edge of the liver.Some branches were even embolized by more than one islets.ConclusionClearly showing of the blood microvasculars in the liver and the station of the islets transplanted into the liver from the rat portal vein can be available by this method, which makes a good foundation for the further study on how to improve the islet survival and the function transplanted in the liver.

Key words rats； decellularized liver bioscaffold； portal vein； islet transplantation

胰島移植作為一種臨床有效的治療胰島素絕對缺乏糖尿病的手段，因其微創和安全性好，已越來越被國內外醫學界推崇[1-2]，五年的臨床移植后結果也已與全胰腺移植無差異[3]?！?br>