人乳頭瘤病毒52型E2和E6／E7在宮頸病變中的表達及其意義

李岳軍　周妙姬　邱峰★

人乳頭瘤病毒52型E2和E6／E7在宮頸病變中的表達及其意義

李岳軍1周妙姬2邱峰1★

［摘要］目的觀察人乳頭瘤病毒52型（human papillomavirus 52，HPV52）E2及E6／E7mRNA在宮頸癌及癌前病變組織中的表達以及高危型HPV分布情況，探討HPV52感染及E2、E6／E7表達在宮頸癌發生發展中的臨床意義。方法收集512例高危型HPV陽性病例，依據病理學診斷歸入非典型鱗狀上皮細胞病變組（atypical squamous cell lesions，ASC）、低度鱗狀上皮內病變組（low grade squamous intraepithelial lesion，LSIL組）、高度鱗狀上皮內病變組（high grade squamous intraepithelial lesion，HSIL）以及鱗狀細胞癌組（squamous cell carcinoma，SCC）。以實時熒光定量PCR方法檢測124例HPV52陽性樣本E2及E6／E7 mRNA，并進行卡方檢驗及Spearman相關性分析。結果HPV52型E2與E6／E7mRNA呈負相關，rs＝－0．98，P＜0．01，兩者檢出率在組間有統計學差異（P＜0．05）。在13種高危型HPV中，檢出率最高前三位為HPV52（24．2%）、HPV58（20．5%）、HPV16（11．7%）。結論HPV52型E2缺失、E6／E7表達過度是宮頸癌變的關鍵環節，E2、E6／E7水平在評估宮頸病變程度中具有參考價值。同時，HPV52型在宮頸癌的發展其作用值得進一步研究。

［關鍵詞］人乳頭瘤病毒52型（HPV52型）；E2；E6／E7；宮頸癌

作者單位：1．廣東省中醫院檢驗科，廣東，廣州510120 2．凱普生物科技有限公司，廣東，潮州521000

Expression of E2 and E6/E7 of human papillomavirus-52 and its significance in cervical lesions

LI Yuejun1, ZHOU Miaoji2, QIU Feng1★
(1. Department of Laboratory Medicine, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou, Guangdong, China, 510120; 2. Hybribio Limited, Chaozhou, Guangdong, China, 521000)

[ABSTRACT]Objective To explore the clinical significance on expressions of E2 and E6／E7 in human papillomavirus 52 in the development of cervical cancer.Methods Based on the pathological features, 512 HR HPV-positive samples were classified into 4 groups which were atypical squamous cell lesions (ASC), low grade squamous intraepithelial lesion(LSIL), high grade squamous intraepithelial lesion (HSIL) and squamous cell carcinoma (SCC). E2 and E6/E7 mRNA were detected among 124 HPV52-positive samples. The statistics were analyzed by Chi-squared test and Spearman correlation method．Results There was negative correlation between expressions of E2 and E6/E7 (rs=－0.98, P<0.01), and there were significantly different positive rates among groups. Among all the 13 common types of HPV, the positive rate of HPV52 was the highest (24.2%), followed by HPV58(20.5%) and HPV16 (11.7%). Conclusion The deletion of HPV52 E2 or over expression of E6/E7 is the key link of cervical cancer, which produce reference value in evaluating the degree of cervical lesions. HPV52 may play a vital role in cervical carcinogenesis, which is worth for further study.

［KEY WORDS］Human papillomavirus 52（HPV52）；E2；E6／E7；Cervical carcinoma

持續的高危型人乳頭瘤病毒（human papillomavirus，HPV）感染在宮頸癌發生發展中起關鍵作用，高危型HPV可從90%以上宮頸癌組織中分離得到，且近年來檢出率有上升趨勢［1］。在大量已有研究中，HPV16型及HPV18型因其在宮頸癌組織中的高檢出率，被認為有高致癌風險，與宮頸癌的發生及發展密切相關，成為研究熱點［2］。近幾年有研究發現HPV52型在亞洲地區檢出率有所上升，研究統計得出其檢出率在世界范圍僅次于HPV16及HPV18型［3－4］。在部分地區，HPV52型在宮頸病變中檢出率在常見高危型HPV中位居第一［5］。……