雞胚地龍接骨膏治療骨折的實驗研究

【摘要】 目的 觀察雞胚地龍接骨膏促進骨折愈合的過程及其機理。方法 將60只新西蘭大白兔制作骨折動物模型，隨機分成三組，治療組20只，于患處外貼雞胚地龍接骨膏，對照組20只，口服傷科接骨片，開放組20只，不使用任何藥物。三組均以相同大小外用繃帶、膠布固定，治療28天。分別于術后7天、術后14天、術后21天、術后28天處死動物，取橈骨標本作X線檢查、抗折力測試、骨折處組織學觀察、骨痂評分并應用微機圖像處理系統作骨痂內骨系細胞立體定量分析。結果 治療組術后21天新西蘭大白兔傷肢完全愈合，骨折線消失，骨髓腔再通，而對照組和開放組動物骨折處未愈合，斷端清晰可見。X線檢查顯示治療組的骨折修復優于對照組和開放組，并且生物力學性能、骨痂評分及骨痂內成骨細胞立體定量均顯著高于對照組和開放組（P＜0.05或＜0.01）。結論 雞胚地龍接骨膏外貼治療能加速促進骨折愈合過程和提高骨折愈合質量。

【關鍵詞】 雞胚地龍接骨膏；骨折；中藥外治法

文章編號：1003-1383(2010)02-0117-04 中圖分類號：R 683.41 文獻標識碼：A

doi:10.3969/j.issn.1003-1383.2010.02.001

Experimental study of the influence of Jipeijiegu ointment for treatment of fracture

SHEN Zhenhua，HU Jianshan，ZHAO Suping，LI Pu，LI Wenzhong，QIN Jiayong，SONG Xillun

(Department of Pathology，The Hosptial of TCM of Qiannan，Duyun 558000，China)

【Abstract】 Objective To observe the effect of Jipeijiegu ointment for the treatment of fracture and explore its mechanism.

Methods Animal fracture models were made in in 60 rabbits.They were divided randomly into treatment group，control group and open group.The treatment group was given JiPeijiegu ointment，while the control group was given shangkejiegutabella，and the open group didn't given medicine. All rabbits were fixed with the same bandageand adhesive tapefor 28 days.The animals were sacrificed respectively in the 7th，14th，21st and the 28th day after treatment，and the radius specimen were examined by Xray，restence test， histological observation，calus score，and made cell stereoquantitative analysis on bone cell lineage within osteotylus by using computer image processing system.Results The wounded limbs repaired completely，fracture disappeared and marrow cavity unobstructed again in treatment group within 21 days after operation，the Xray films suggested that the fracture healing of the treatment group were better than that of the control group and the open group.the result of biomechanical property tested and integrate intensity of callus by Xray film and bone cell lineage within osteotylus by using computer image processing system of the treatment group were significantly better than that of the control group and the open group(P＜0.05 or P＜0.01).Con

clusion Jipeijiegu ointment can promote fracture healing and improve fracture healing quality.

【Key words】 Jipeijiegu ointment；fracture；TCM external use

骨折是臨床上常見的病癥，嚴重威脅患者的健康和生活質量，甚至導致殘廢和死亡。中醫中藥治療促進骨折愈合療效肯定，已被臨床廣泛證實。但大多偏向于內服中藥方面的研究，而外用中藥促進骨折愈合的研究較少。雞胚地龍接骨膏為我院臨床應用20多年治療骨折的外用中藥制劑，具有活血祛瘀、消腫止痛、續筋接骨之功效。我們選擇新西蘭大白兔制作骨折動物模型，采用雞胚地龍接骨膏外用治療，觀察其促進骨折愈合的療效并探討其作用機理，現將實驗結果報告如下。……