SCF/c-kit信號通路在肝內膽汁淤積癥中與顆粒細胞凋亡的關系

【摘要】 目的：探討血清干細胞因子（SCF）/酪氨酸蛋白激酶生長因子受體（c-kit）信號通路在肝內膽汁淤積癥中與顆粒細胞凋亡的關系。方法：將黑龍江省醫院普外三科2017年3月—2020年12月收治的肝內膽汁淤積癥患者41例納入研究，記作病變組，另取同期健康受試者41例作為健康組。分別比較兩組顆粒細胞凋亡情況，血清SCF、c-kit表達水平，顆粒細胞內Bcl-2及Caspase-3 mRNA轉錄水平，并作相關性分析。結果：病變組顆粒細胞凋亡細胞DNA含量、血清SCF、c-kit表達水平、Caspase-3 mRNA轉錄水平均高于健康組（Plt;0.05），病變組顆粒細胞內Bcl-2 mRNA轉錄水平低于健康組（Plt;0.05）。經Pearson相關性分析可得：肝內膽汁淤積癥患者血清SCF、c-kit表達水平與顆粒細胞內Bcl-2 mRNA轉錄水平呈負相關（r=-0.532、-0.583，P=0.001、0.000），而與Caspase-3 mRNA轉錄水平呈正相關（r=0.479、0.510，P=0.001、0.004）。結論：在肝內膽汁淤積癥中，SCF/c-kit信號通路可能和顆粒細胞凋亡密切相關，兩者之間的聯系可能是通過Bcl-2、Caspase-3的表達實現。

【關鍵詞】 肝內膽汁淤積癥 酪氨酸蛋白激酶生長因子受體 干細胞因子 顆粒細胞

Relationship between SCF/c-kit Signaling Pathway and Granulosa Cell Apoptosis in Intrahepatic Cholestasis/SUN Yu， GE Xin. //Medical Innovation of China， 2023， 20（31）： 00-004

[Abstract] Objective： To investigate the relationship between serum stem cell factor （SCF）/tyrosine protein kinase growth factor receptor （c-kit） signaling pathway and granulosa cell apoptosis in intrahepatic cholestasis. Method： A total of 41 patients with intrahepatic cholestasis admitted to the Third Department of General Surgery of Heilongjiang Provincial Hospital from March 2017 to December 2020 were included in the study and recorded as the diseased group， and 41 healthy subjects in the same period were selected as the healthy group. The apoptosis of granulosa cells， the expression levels of serum SCF and c-kit， the mRNA transcription levels of Bcl-2 and Caspase-3 in granulosa cells were compared between the two groups， and the correlation was analyzed. Result： The DNA content of apoptotic granulosa cells， expression levels of serum SCF， c-kit and the mRNA transcription of Caspase-3 in the diseased group were higher than those in the healthy group （Plt;0.05）， the mRNA transcription level of Bcl-2 in granulosa cells of the diseased group was lower than those of the healthy group （Plt;0.05）. Pearson correlation analysis showed that the expression levels of SCF and c-kit in serum of patients with intrahepatic cholestasis were negatively correlated with the transcription level of Bcl-2 mRNA in granular cells （r=-0.532， -0.583; P=0.001， 0.000）， but positively correlated with Caspase-3 mRNA transcription level （r=0.479， 0.510; P=0.001， 0.004）. Conclusion： SCF/c-kit signaling pathway may be closely related to granulosa cell apoptosis in intrahepatic cholestasis， and the relationship between them may be mediated by the expression of Bcl-2 and Caspase-3.

[Key words] Intrahepatic cholestasis Tyrosine protein kinase growth factor receptor Stem cell factors Granulosa cells

First-author's address： Heilongjiang Provincial Hospital， Harbin 150000， China

doi：10.3969/j.issn.1674-4985.2023.31.001

肝內膽汁淤積癥屬于臨床上較為常見的疾病之一，嚴重威脅患者的生命健康安全[1]。有研究報道指出，肝內膽汁淤積癥的發病機制可能和遺傳、生活方式及細胞異常刺激等密切相關[2]。其中顆粒細胞于細胞發育、成熟等過程中起著至關重要的作用，可能參與了肝內膽汁淤積癥的發生、發展過程[3]。此外，血清干細胞因子（stem cell factor，SCF）是一種多功能細胞生長因子，參與機體多種細胞生長的調控[4]。而酪氨酸蛋白激酶生長因子受體（c-kit）蛋白細胞外的部分為血清SCF的受體區，細胞膜、細胞漿為酪氨酸激酶區[5]。……