加味獨(dú)活寄生合劑對兔膝骨關(guān)節(jié)炎顯微結(jié)構(gòu)的影響

黃璐　譚旭儀　鄺高艷　盧敏

【摘 要】目的：觀察加味獨(dú)活寄生合劑對兔膝骨關(guān)節(jié)炎顯微結(jié)構(gòu)的影響。方法：采用隨機(jī)數(shù)字表法將80只白兔分為正常對照組、模型對照組、陽性對照組和獨(dú)活寄生組，每組20只。除正常對照組外，其余組均成功制備KOA模型。獨(dú)活寄生組給予加味獨(dú)活寄生合劑6.8 mL·kg^-1·d^-1灌胃，陽性對照組給予鹽酸氨基葡萄糖膠囊0.07 g·kg^-1·d^-1灌胃，正常對照組和模型對照組給予等劑量生理鹽水灌胃。在實(shí)驗(yàn)干預(yù)前、干預(yù)第2，4，8周時，對關(guān)節(jié)軟骨形態(tài)及顯微結(jié)構(gòu)進(jìn)行觀察，并采用番紅固綠染色進(jìn)行Mankins關(guān)節(jié)軟骨評分。結(jié)果：與模型對照組比較，獨(dú)活寄生組膝關(guān)節(jié)大體觀察得到有效改善。模型對照組、陽性對照組、獨(dú)活寄生組Mankins軟骨評分在干預(yù)前和干預(yù)第2，4，8周均高于正常對照組，且陽性對照組、獨(dú)活寄生組Mankins軟骨評分在干預(yù)第2，4，8周均低于模型對照組，差異有統(tǒng)計(jì)學(xué)意義（P < 0.05）。結(jié)論：加味獨(dú)活寄生合劑可有效改善KOA關(guān)節(jié)軟骨顯微結(jié)構(gòu)，這可能是其治療KOA的作用機(jī)制。

【關(guān)鍵詞】 膝骨關(guān)節(jié)炎;加味獨(dú)活寄生合劑;軟骨;顯微結(jié)構(gòu);實(shí)驗(yàn)研究;白兔

Effect of Jiawei Duhuo Jisheng Heji（加味獨(dú)活寄生合劑）on Microstructure of Rabbit Knee Osteoarthritis

HUANG Lu，TAN Xu-yi，KUANG Gao-yan，LU Min

【ABSTRACT】Objective：To observe the effect of Jiawei Duhuo Jisheng Heji（加味獨(dú)活寄生合劑）

on the microstructure of rabbit knee osteoarthritis（KOA）.Methods：Eighty white rabbits were randomly divided into a normal control group，a model control group，a positive control group and a Duhuo Jisheng group，with

20 rabbits in each.KOA models were successfully prepared in all groups except the normal control group.The Duhuo Jisheng group was given 6.8 mL·kg^-1·d^-1of Jiawei Duhuo Jisheng Heji by gavage，the positive control group was given 0.07 g·kg^-1·d^-1of glucosamine hydrochloride capsule by gavage，and the normal control group and model control group were given the same dose of normal saline by gavage.The morphology and microstructure of articular cartilage were observed before the experimental intervention and at the 2nd，4th and 8th weeks of the intervention，and the Mankins articular cartilage score was performed by safranine solid green staining.Results：Compared with the model control group，by general observation the knee joint in the Duhuo Jisheng group was effectively improved，Mankins cartilage in model control group，positive control group，Angelica pubescens parasitic group.The scores were higher than those of normal control before intervention and at 2，4 and?8 weeks after intervention Group，and mankins cartilage score of positive control group and Angelica pubescens parasitic group.At 2，4，and 8 weeks of intervention，it was lower than that in the model control group，the difference being statistically significant（P < 0.05）.Conclusion：Jiawei Duhuo Jisheng Heji can effectively improve the microstructure of KOA articular cartilage，which may be the mechanism of its treatment of KOA.

【Keywords】 knee osteoarthritis;Jiawei Duhuo Jisheng Heji（加味獨(dú)活寄生合劑）;cartilage;microstructure;

experimental research;white rabbit

膝骨關(guān)節(jié)炎（knee osteoarthritis，KOA）是一種以關(guān)節(jié)軟骨的變性、破壞及骨質(zhì)增生為特征的慢性關(guān)節(jié)病，系軟骨細(xì)胞、細(xì)胞外基質(zhì)以及軟骨下骨三者降解與合成偶聯(lián)失衡的結(jié)果，故軟骨的退變與本病的發(fā)生、發(fā)展密切相關(guān)[1-5]。筆者前期提出KOA存在“虛、瘀、毒”病機(jī)特點(diǎn)，以此組方而成的加味獨(dú)活寄生合劑對KOA臨床驗(yàn)效頗佳[6-14]。本研究擬觀察加味獨(dú)活寄生合劑對KOA軟骨顯微形態(tài)的影響，進(jìn)一步探討其對KOA的作用機(jī)制，現(xiàn)總結(jié)如下。……