化學(xué)發(fā)光法和酶聯(lián)免疫法在乙型肝炎病毒血清學(xué)檢驗(yàn)中的效果比較

唐細(xì)田，肖琳，徐彤慧

摘要：目的 ?比較化學(xué)發(fā)光法和酶聯(lián)免疫法在乙型肝炎病毒血清學(xué)檢驗(yàn)中的效果。方法 ?選取2017年2月～2019年5月我院收治的疑似乙型肝炎患者63例作為研究對象，均予以酶聯(lián)免疫法、化學(xué)發(fā)光法進(jìn)行血清學(xué)檢驗(yàn)，以熒光定量PCR為金標(biāo)準(zhǔn)，比較兩種檢驗(yàn)方式準(zhǔn)確性、血清學(xué)指標(biāo)檢出率。結(jié)果 ?共63例疑似乙肝患者，熒光定量PCR檢驗(yàn)陽性38例、陰性25例;酶聯(lián)免疫法和化學(xué)發(fā)光法中準(zhǔn)確度、靈敏度、特異度比較，差異無統(tǒng)計(jì)學(xué)意義（P>0.05）。38例乙肝陽性患者中，酶聯(lián)免疫法HBsAb、HBcAb陽性檢出率與化學(xué)發(fā)光法比較，差異無統(tǒng)計(jì)學(xué)意義（P>0.05）;酶聯(lián)免疫法HBeAb、HBeAg、HBsAg陽性檢出率低于化學(xué)發(fā)光法，差異有統(tǒng)計(jì)學(xué)意義（P<0.05）。結(jié)論 ?乙型肝炎病毒血清學(xué)檢驗(yàn)中，采用酶聯(lián)免疫法、化學(xué)發(fā)光法均具良好診斷效果，但在血清標(biāo)志物檢驗(yàn)中，化學(xué)發(fā)光法檢出效果更佳，有利于保證臨床診斷準(zhǔn)確性。

關(guān)鍵詞：酶聯(lián)免疫法;化學(xué)發(fā)光法;乙型肝炎病毒;血清學(xué)檢驗(yàn)

中圖分類號：R512.62;R446.6 ? ? ? ? ? ? ? ? ? ? ? ? ?文獻(xiàn)標(biāo)識碼：A ? ? ? ? ? ? ? ? ? ? ? ? ? ?DOI：10.3969/j.issn.1006-1959.2019.22.057

文章編號：1006-1959（2019）22-0164-02

Comparison of the Effects of Chemiluminescence and Enzyme-linked Immunosorbent Assay

on Serological Test of Hepatitis B Virus

TANG Xi-tian，XIAO Lin，XU Tong-hui

（Department of Clinical Laboratory，Xiajiang County People's Hospital，Xiajiang 331409，Jiangxi，China）

Abstract：Objective ?To compare the effects of chemiluminescence and enzyme-linked immunosorbent assay on the serological test of hepatitis B virus. Methods ?A total of 63 patients with suspected hepatitis B admitted to our hospital from February 2017 to May 2019 were selected as subjects.Evidence-linked immunosorbent assay and chemiluminescence assay were used for serological tests. Fluorescence quantitative PCR was used as the gold standard. The accuracy of the two test methods and the detection rate of serological markers were compared. Results ?A total of 63 patients with suspected hepatitis B were positive for fluorescent quantitative PCR and 38 for negative，there were 25 cases of negative; there was no significant difference in accuracy， sensitivity and specificity between enzyme-linked immunosorbent assay and chemiluminescence method （P>0.05）. In 38 cases of hepatitis B positive patients， the positive detection rate of HBsAb and HBcAb by enzyme-linked immunosorbent assay was not significantly different from that of chemiluminescence method （P>0.05）. The positive detection rate of HBeAb， HBeAg and HBsAg was lower than that of enzyme-linked immunosorbent assay. The chemiluminescence method was statistically significant （P<0.05）. Conclusion ?In the serological test of hepatitis B virus， both enzyme-linked immunosorbent assay and chemiluminescence method have good diagnostic results. However， in the serum marker test， the chemiluminescence method has better detection effect， which is beneficial to ensure the accuracy of clinical diagnosis.