鼠李糖乳桿菌LGG 細胞壁蛋白的預測和功能分析

朱德全 高峰 姚嘉 張躍華 韓誠武 盧偉 欒積毅 劉向東

摘要 [目的]采用生物信息學方法預測鼠李糖乳桿菌LGG細胞壁蛋白和功能分析。[方法]以鼠李糖乳桿菌LGG基因組編碼蛋白序列為研究對象，采用Phobius和SignalP 4.0軟件分析該菌株的細胞壁蛋白，同時采用COG功能數據庫對預測的細胞壁蛋白進行功能分析。[結果]鼠李糖乳桿菌LGG基因組中含有41個細胞壁蛋白，這些蛋白的功能分析結果顯示，41個細胞壁蛋白中，25個蛋白沒有功能注釋，16個有功能注釋，主要與細胞壁和細胞膜的生物合成，碳水化合物的代謝與轉運，蛋白質的翻譯后修飾等功能有關。[結論]該研究從結構和功能上分析鼠李糖乳桿菌細胞壁蛋白，為分析益生菌適應環境的分子特征打下基礎。

關鍵詞 鼠李糖乳桿菌LGG；基因組；細胞壁蛋白；功能

中圖分類號 S188+.2 文獻標識碼 A 文章編號 0517-6611（2017）13-0141-02

Prediction and Functional Analysis of the Cell Wall Proteins in Lactobacillus rhamnosus LGG

ZHU De-quan1， GAO Feng2， YAO Jia3， LIU Xiang-dong3* et al

（1.College of Science， Jiamusi University， Jiamusi， Heilongjiang 154007；2.College of Life Science， Jiamusi University， Jiamusi， Heilongjiang 154007；3.College of Mechanical Engineering， Jiamusi University， Jiamusi， Heilongjiang 154007）

Abstract [Objective]Cell wall proteins of Lactobacillus rhamnosus LGG were predicted and analyzed by bioinformatics. [Method]Cell wall proteins sequences of L. rhamnosus LGG were analyzed by SignalP 4.0 and Phobius software in this paper. At the same time， the functional annotation and clustering analysis of these predicted proteins were performed by COG functional database. [Result] There were 41 cell wall proteins which possessed cell wall motif. Function analysis of these proteins revealed that there were 25 proteins which have no function annotation and 16 proteins which have concrete function annotation. Function of these proteins was mainly involved in cell wall/membrane biogenesis， posttranslational modification， and metabolism and transport of carbohydrate， etc. [Conclusion] Structure and function of cell wall proteins of L. rhamnosus LGG were analyzed， which will provide the basic data for analyzing molecular characteristics of this strain to adopt environment.

Key words Lactobacillus rhamnosus LGG；Genome； Cell wall proteins；Function

LGG屬于乳桿菌屬鼠李糖乳桿菌種，腸道黏附率高，具有調節腸道菌群、預防和治療腹瀉[1]、排除毒素、預防齲齒、提高機體免疫力等益生功能[1-3]。

鼠李糖乳桿菌LGG周圍有一層厚厚的細胞壁，而細胞壁蛋白是通過特殊的錨定基序錨定在細胞壁上，如含有LPxTG結構的序列、LysM、PG等結合區域。細胞壁蛋白在適應外界環境并與外界環境發生作用時扮演重要的作用[4]。2009年該菌株完成了全基因組測序，該菌株基因組全長為3.01 Mb，2 703個編碼蛋白。該菌株全基因組測序的完成[5]使得利用基因組數據進行功能基因組學分析得以實現，如細胞壁蛋白的基因組預測和分析。……