外生菌對植物光合日變化的通徑分析

申慧慧 畢銀麗 王志剛 趙新偉 莊超然

摘要 [目的]揭示樟子松光合日變化特征及影響因子之間的作用關(guān)系。[方法]通過研究4年樹齡的樟子松光合日變化特征，設(shè)置接種彩色豆馬勃菌[Pisolithus tinctorius（Pers.）Coker & Couch]（接菌組）和不接菌（對照組）2種處理，采用便攜式光合測定儀測定樟子松08∶00—18∶00的凈光合速率（Pn）及其參數(shù)，研究其光合日變化特征。[結(jié)果]對照組和接菌組的Pn日變化繪制的都是“雙峰”曲線。對照組的Pn是0.89～6.67 μmol/（m2·s），接菌組的Pn是0.51～10.90 μmol/（m2·s），最小值均出現(xiàn)在18∶00，最大值均出現(xiàn)在09∶00。接菌組Pn的日均值顯著大于對照組。影響Pn的環(huán)境因子存在共線關(guān)系。得到多元逐步線性回歸方程，對照組：Pn=-120.96+63083Cond -0.157Ta+8.06Ls +0.001PAR -0.05Ca+1.644RH（R2=82.9，F(xiàn)=161 279.83）；接菌組：Pn=5 910.823+92.104Cond-11807Ta +12.622Ls-0.006PAR（R2=0.92.7，F(xiàn)=2 045.23）。通過通徑分析得到對照組環(huán)境因子對Pn的通徑系數(shù)（直接作用）的順序是Cond>Ta>Ls>PAR>Ca>RH，間接通徑系數(shù)（間接作用）的順序是RH>PAR>Ta>Ca>Ls>Cond；接菌組環(huán)境因子對Pn的通徑系數(shù)大小順序是Cond>Ls>PAR>Ta；間接通徑系數(shù)順序是Ls>PAR>Cond>Ta。接菌組RH、Ca不是限制因子。[結(jié)論]試驗結(jié)果為不同處理下影響Pn的主導(dǎo)因素研究提供了參考。

關(guān)鍵詞 樟子松；光合日變化；彩色豆馬勃菌；多元線性回歸；通徑分析

中圖分類號 X171.1 文獻(xiàn)標(biāo)識碼 A 文章編號 0517-6611（2017）13-0001-03

Path Analysis of Diurnal Variation of Photosynthesis in Plants with Ectomycorrhial Fungi—For Example Pinus sylvestris var. mongolica Litv.

SHEN Hui-hui， BI Yin-li*， WANG Zhi-gang et al

（State Key Laboratory of Coal Resources and Safe Mining， China University of Mining and Technology （Beijing）， Beijing 100083）

Abstract [Objective] The aim was to reveal the relationship between diurnal variation of photosynthesis in Pinus sylvestris and impact factors. [Method] We used portable Pn instrument to determinate the net photosynthetic rate and its parameters of 4 years old Pinus sylvestris var. mongolica between 08∶00 and 18∶00， and studied the photosynthetic diurnal variation characteristics of natural group and inoculated with Pisolithus tinctorius group. [Result] The photosynthetic diurnal variation characteristics of the control group and the bacteria group were “bimodal curve”. The Pn range of the control group was 0.89-6.67 μmol/（m2·s）， and the range of Pn was 0.51-10.90 μmol/（m2·s）；the minimum value appeared at 18∶00， and the maximum value appeared at 09∶00. There were collinear relationship of the environmental factors that effected Pn. Multiple linear regression equations for the control group was： Pn = -120.96 +63.083Cond -0.157Ta +8.06Ls+0001PAR-0.05Ca+1.644RH（R2=0.992，F(xiàn)=161 279.83）， and the inoculated with Pisolithus tinctorius group was： Pn=5 910.823+92104Cond-11.807Ta +12.622Ls-0.006PAR（R2=0.997，F(xiàn)=2 045.23）. Through path analysis about the impact of environmental factors on net photosynthesis rate could get the order of path coefficient for the control group was Cond>Ta>Ls>PAR>Ca>RH， and the order of indirect path coefficient was RH>PAR>Ta>Ca>Ls>Cond；the order of path coefficient for the inoculated with Pisolithus tinctorius group was Cond>Ls>PAR>Ta， and the order of indirect path coefficient was Ls>PAR>Cond>Ta. The RH and Ca of Pisolithus tinctorius group were not limiting factors. [Conclusion] The results provide reference for study on dominant impact factor of Pn under different treatments.

Key words Pinus sylvestris var. mongolica Litv.；Photosynthetic diurnal variation；Pisolithus tinctorius （Pers.） Coker & Couch；Multiple linear regression；Path analysis

光合作用是植物最基本的生命活動，對植物光合的研究勢必成為人類的重要課題[1]。在陜西省神木縣大柳塔鎮(zhèn)，采煤活動造成的沉陷地土壤結(jié)構(gòu)不良，水分短缺，養(yǎng)分貧瘠，原有立地條件遭到極大破壞[2]。改善采煤沉陷地的植物及其功能成為迫在眉睫的任務(wù)[3-4]。樟子松與外生菌根菌聯(lián)系十分緊密，在自然狀態(tài)下，樟子松在沒有外生菌根的狀態(tài)下是無法生存的[5]。自1955年在科爾沁沙地南緣成功人工引種樟子松造林以來[5]，樟……