影響獺兔離體腸道平滑肌收縮的因素

摘要[目的]探討影響獺兔離體腸道平滑肌收縮的因素。[方法]以獺兔十二指腸平滑肌為試驗材料，以平滑肌收縮波的振幅和周期為評價指標，考察影響試驗的各主要因素。[結果]在（36.5±0.5） ℃臺氏液中，獺兔離體腸管的存活時間和保質期最長，振幅最穩定，腎上腺素使離體腸管平滑肌收縮幅度減弱，乙酰膽堿、氫氧化鈉及氯化鈣均導致平滑肌收縮頻率及幅度升高，收縮力增強。[結論]獺兔離體腸道平滑肌易受到溫度、pH、其他化學因素及藥物的影響。

關鍵詞獺兔；離體試驗；十二指腸；平滑肌

中圖分類號S829.1文獻標識碼

A文章編號0517-6611（2017）10-0101-03

Influencing Factors on the Contraction of Smooth Muscle in the Isolated Intestines of Rex Rabbit

DING Wenkai（Animal Husbandry and Veterinary Station in Ganzihe Township，Haiyan County of Qinghai Province，Haiyan，Qinghai 812299）

Abstract[Objective] To discuss the influencing factors on the contraction of smooth muscle in isolated intestines of rex rabbit. [Method] Taking smooth muscle in the duodenum of rex rabbit as test materials， using contractive waves amplitude and cycle of smooth muscle as evaluation indices， their main influencing factors were explored. [Result] In （36.5±0.5） ℃ Tyrode solution， isolated intestines of rex rabbit had the longest survival time and shelf life， and the most stable amplitude. The smooth muscles contraction amplitude of isolated intestines was weakened by epinephrine. The contraction frequency and amplitude of smooth muscle were increased by acetylcholine， sodium hydroxide and calcium chloride， and the contraction force was enhanced by acetylcholine， sodium hydroxide and calcium chloride. [Conclusion] The smooth muscle of isolated intestines of rex rabbit is easily affected by temperature， pH， other chemical factors and drugs.

Key wordsRex rabbit； Experiment in vitro；Duodenum；Smooth muscle

胃腸動力學異常性疾病是引起消化道疾病的重要影響因素之一，在國內發病率較高。離體腸道平滑肌試驗能為研究藥物對胃腸道的作用機制提供有效手段。筆者考察影響獺兔離體腸道平滑肌試驗的主要因素及評價指標，考察影響試驗的各主要因素的優缺點。

1材料與方法

1.1試驗動物從青海省湟源縣民興獺兔場購買健康無病

的2月齡獺兔20只（雌雄各半），試驗在青海畜牧獸醫職業

技術學院中心實驗室完成。

1.2試驗儀器與試劑

試驗儀器有BL-420F生物信號采集處理系統、JD-400恒溫平滑肌槽和FT-100張力換能器均購自成都泰盟科技有限公司；試驗試劑為氧氣袋、常用代體液（見表1）、0.01%腎上腺素、0.01%乙酰膽堿、雙蒸水，其他試劑均為分析純。

1.3試驗裝置和標本制備

為保障離體腸道的運動效果，獺兔禁食24 h后，試驗前1 h飼喂青草和兔糧，自由飲水。用木棒猛擊……