丹參多酚酸鹽對大鼠腦缺血再灌注過氧化損傷的保護作用

【摘要】 目的 觀察丹參多酚酸鹽對大鼠局灶性腦缺血再灌注損傷后腦組織內SOD、GSHPx和MDA含量的影響，探討其缺血腦保護的作用機制。

方法線栓法制作大鼠中動脈缺血再灌注損傷模型。SD大鼠隨機分為正常對照組、缺血再灌注組、丹參多酚酸鹽低劑量治療組（10 mg/kg）和丹參多酚酸鹽高劑量治療組（30 mg/kg）。各組動物按要求給藥，在預定時間進行神經損傷行為學評分、腦梗死體積及腦組織內超氧化物歧化酶（SOD）、谷胱甘肽過氧化物酶（GSHPx）活性和丙二醛（MDA）含量的測定。結果 與缺血再灌注組相比，丹參多酚酸鹽治療組大鼠神經損傷行為學評分及腦梗死體積減少，腦組織內SOD、GSHPx活性升高，MDA含量下降，均具有統計學意義(P＜0.05)，且呈劑量依賴性。結論丹參多酚酸鹽可通過抑制脂質過氧化反應，保護抗氧化酶的活性，從而介導神經保護作用。

【關鍵詞】 丹參多酚酸鹽；腦缺血再灌注；超氧化物歧化酶；谷胱甘肽過氧化物酶；丙二醛

文章編號：1003-1383(2010)06-0665-03 中圖分類號：R 743文獻標識碼：A

doi:10.3969/j.issn.1003-1383.2010.06.005

The effect of salvianolate on the peroxidation injury induced by focal

cerebral ischemia reperfusion in rats

WANG Qiang1，ZHANG Yi1，LI Lu2，DONG Bo1，YANG Yilin1

(1.Department of Neurosurgery，2.Department of Clinical Laboratory，

Changzhou First People's Hospital，Changzhou 213003，China)

【Abstract】 ObjectiveTo study the protective effect of salvianolate on the peroxidation injury induced by focal cerebral ischemia reperfusion in rats and to reveal the mechanism.

Methods 48 SD rats were divided into four group randomly，they are normal control group，ischemia reperfusion group，salvianolate (10 mg/kg) group and salvianolate(30 mg/kg)group.Each group contained 12 rats.The Longa method was used to establish the MCAO model.The salvianolate at different dose were injected intraperitoneally at different time point according to the test design. The brain infarct volume by TTC stain and behavior scores by Bederson's methods were also determined in order to evaluated the protective effect.Also we use assay kit to determine the activities of SOD and GSHPx，content of MDA in each group.

Results Comparing with the ischemia reperfusion group， brain infarct volume and behavior score of salvianolate group was lower，the activities of SOD and GSHPx of brain tissue increased，the content of MDA decreased.There was significant difference between them(P＜0.05)，the effect of salvianolate was dosedependent.

Conclusion The salvianolate plays neuroprotective effect by inhibiting the lipid peroxidation and protect the activity of antioxidant enzyme.

【Key words】salvianolate；cerebral ischemia reperfusion；SOD；GSHPx；MDA

大量證據表明氧自由基引起的脂質過氧化是腦缺血再灌注損傷的重要機制之一。缺血再灌注可使自由基連鎖反應激化，大量的自由基引起細胞膜脂質過氧化，導致神經元死亡，造成嚴重的神經功能損害。丹參是一種傳統的活血化瘀中藥，在臨床上被廣泛用于治療冠心病、心絞痛、缺血性中風等疾病，丹參多酚酸是其最重要的有效成分［1］。已有實驗證實丹參多酚酸對實驗性腦缺血具有顯著的保護作用［2］，但其機制尚不明確。……