E-cadherin與Snail在涎腺腺樣囊性癌中的表達(dá)及意義

[摘要] 目的 研究E-cadherin與Snail 在人腺樣囊性癌(SACC)中的表達(dá)及臨床意義。方法 用免疫組化的方法檢測E-cadherin與Snail在SACC及正常涎腺組織中的表達(dá)。結(jié)果 (1)與正常涎腺組織相比，E-cadherin在SACC中表達(dá)下降，差異有統(tǒng)計(jì)學(xué)意義(P<0.05)，E-cadherin表達(dá)與不同組織病理學(xué)類型有關(guān)，且在發(fā)生神經(jīng)侵犯及遠(yuǎn)處轉(zhuǎn)移的病例中，E-cadherin表達(dá)下降更明顯(P<0.05，P<0.01)。(2)Snail在SACC 中表達(dá)，且Snail表達(dá)與組織病理學(xué)類型及生物學(xué)行為有關(guān)，在實(shí)體型及發(fā)生神經(jīng)侵犯及遠(yuǎn)處轉(zhuǎn)移的病例中，Snail表達(dá)明顯增加(P<0.001，P<0.05，P<0.05)。(3)在SACC， E- cadherin與Snail的表達(dá)呈負(fù)相關(guān)(r=-0.496，P<0.001)。結(jié)論 Snail與E-cadherin的聯(lián)合檢測可作為SACC惡性程度及預(yù)后的參考指標(biāo)。

[關(guān)鍵詞] E-cadherin;Snail;腺樣囊性癌

[中圖分類號] R781.7 [文獻(xiàn)標(biāo)識碼] A [文章編號] 1673-9701(2010)04-14-03

Expression of E-cadherin and Snail in Salivary Adenoid Cystic Carcinoma and Its Significance

LI YuwangNAN XinrongLINGHU Qingxi

The First Affiliated Hospital of Shanxi Medical College，Taiyuan 030001，China

[Abstralt] ObjectiveTo study the expression of E-cadherin and Snail in salivary adenoid cystic carcinoma and its significance. Methods The expression of E-cadherin and Snail was detected by the immunohistochemical method in salivary adenoid cystic carcinoma and normal salivary gland tissue. Results (1)The expression rate of E-cadherin in salivary adenoid cystic carcinoma was lower than that in normal salivary gland tissue(P<0.05). The E-cadherin expression was associated with different histopathological types and was lower in nerve invasion anddistant metastasis(P<0.05，P<0.01). (2)The Snail was expressed in salivary adenoid cystic carcinoma and its expression was related to histopathological type and biological behaviour. The positive rate of Snail was significantly higher in entity type， nerve invasion and distant metastasis(P<0.001，P<0.05，P<0.05). (3)There was a negative relation between E-cadherin expression and Snail expression in salivary adenoid cystic carcinoma. Conclusion The combined detection of E-cadherin and Snail may be used as a reference indicator of m alignant degree and prognosis.

[Key words] E-cadherin;Snail;Salivary adenoid cystic carcinoma

人涎腺腺樣囊性癌(salivary adenoid cystic carcinoma，SACC)是最常見的口腔頜面部惡性腫瘤，侵襲性強(qiáng)，早期即可侵犯血管與神經(jīng)，易發(fā)生遠(yuǎn)處轉(zhuǎn)移。E-cadherin普遍存在于各類上皮細(xì)胞，介導(dǎo)同型細(xì)胞連接，在維持正常上皮細(xì)胞形態(tài)、細(xì)胞極性及組織結(jié)構(gòu)完整性中發(fā)揮重要作用。Snail是重要的轉(zhuǎn)錄抑制因子，在原腸胚形成中，Snail通過轉(zhuǎn)錄抑制作用，降低E-cadherin蛋白的表達(dá)，促進(jìn)細(xì)胞脫落并遷移，從而在胚胎發(fā)育中發(fā)揮作用[1]。在惡性腫瘤的侵潤、轉(zhuǎn)移中可能存在相似的機(jī)制。我們的研究將探討E-cadherin與Snail在SACC中的表達(dá)及臨床意義。

1材料與方法

1.1組織標(biāo)本收集

選取2000年9月～2009年5月山西醫(yī)科大學(xué)第一臨床醫(yī)院因原發(fā)病手術(shù)切除并經(jīng)病理診斷為SACC患者51例，選取正常涎腺組織15例(來源于同期我院頸淋巴結(jié)清除患者)，51例SACC患者中，按WHO(2004年)的涎腺腫瘤分類標(biāo)準(zhǔn)，篩狀型22例、管狀型19例、實(shí)體型10例。每例腫瘤標(biāo)本至少選取2 張來自不同部位的HE染色切片于光鏡下觀察。……