[摘要] 目的 觀察中介素1-53對(duì)大鼠肺缺血再灌注損傷(LIRI)肺組織過(guò)氧化物酶增殖物激活受體γ(PPAR-γ)的影響。方法 將健康Wistar 大鼠54 只隨機(jī)分為手術(shù)對(duì)照組(C組)、缺血再灌注組(IR組)、中介素干預(yù)組(D組)。每組分別在缺血45min、再灌注60min、120 min 3個(gè)時(shí)點(diǎn)處死6 只大鼠,觀察肺組織病理形態(tài)變化,觀察各組肺濕干質(zhì)量比值(W/D)、CINC-1含量、PPAR-γmRNA、PPAR-γ蛋白的改變。結(jié)果 與IR組比較,中介素1-53預(yù)處理能夠明顯升高PPAR- γmRNA(P<0.05),減輕肺水腫,并降低CINC-1蛋白含量(P<0.05)。結(jié)論 中介素1-53能上調(diào)PPAR-γ的表達(dá),可能通過(guò)PPAR-γ的抗炎作用對(duì)LIRI起保護(hù)作用。
[關(guān)鍵詞] 肺缺血再灌注損傷;中介素1-53;過(guò)氧化物酶體增殖物激活-γ;保護(hù)作用
[中圖分類號(hào)] R364.1+2;R563 [文獻(xiàn)標(biāo)識(shí)碼] A [文章編號(hào)] 1673-9701(2010)04-03-03
Effects of Intermedin1-53 on Pulmonary Peroxisome Proliferator-activated Receptor-γ Expression in Rat Lung Ischemia-reperfusion Injury
LIU NiLI JianqiangYANG ShujuanZHAO Hui
Department of Respiratory,the Second Affiliated Hospital of Shanxi Medical University,Taiyuan 030001,China
[Abstract] Objective To investigate the effect of intremedin 1-53 on peroxisome proliferator-activated receptor-γ(PPAR-γ) in rat lung ischemia-reperfusion injury. Methods Fifty-four healthy Wistar rats were randomly divided into three groups:surgical control group(C group), ischemia-reperfusion group(IR group)and intermedin-pretreated group(D group). At three time points(45 min ischemia,60 min and 120 min reperfusion),6 rats from each group were killed,the lung histopathological changes were observed and the wet /dry weight ratio of lung tissue,CINC-1 content,PPAR-γmRNA and PPAR-γprotein in each group were determined. Results Compared with IR group,the pre-treatment by intermedin1-53 significantly increased PPAR-γmRNA(P<0.05),and decreased interstital edema and CINC-1content(P<0.05). Conclusion Intermedin1-53 may upregulate PPAR-γexpression,which has protective effects on rat lung ischemia- reperfusion injury.
[Key words] Lung ischemia-reperfusion injury;Intermedin1-53;Peroxisome proliferator- activated receptorγ;Protective effects
中介素(IMD)是2004年由Roh等[1]用種系特征方法分析基因庫(kù),從人類和其他脊椎動(dòng)物基因組證實(shí)的一種新的降鈣素(CT)/降鈣素基因相關(guān)肽(CGRP)家族肽.IMD前體蛋白可被內(nèi)源性肽酶降解為prepro-IMD95-147即IMD1-53,目前國(guó)內(nèi)外有報(bào)道中介素1-53對(duì)油酸致急性肺損傷和心肌缺血再灌注損傷以及腎臟缺血再灌注損傷有保護(hù)作用[2-4],但中介素1-53對(duì)肺缺血再灌注損傷的研究尚未見報(bào)道。過(guò)氧化物酶體增殖物激活受體γ(PPAR-γ)屬于細(xì)胞核激素受體超家族的成員,在抗炎和免疫調(diào)節(jié)等方面有重要作用。本課題擬通過(guò)建立大鼠在體肺缺血再灌注模型,觀察肺組織PPAR-γ表達(dá)情況,旨在探討外源性人工合成的IMD1-53對(duì)肺缺血再灌注損傷的作用及其可能機(jī)制。
1材料與方法
1.1材料
1.1.1實(shí)驗(yàn)動(dòng)物健康雄性Wistar大鼠54只(山西醫(yī)科大學(xué)動(dòng)物中心提供),體質(zhì)量220~300g。
1.1.2主要試劑合成的大鼠中介素1-53(IMD1-53)(美……