阿折地平對自發性高血壓大鼠心肌細胞凋亡的影響

[摘要] 目的 探討阿折地平對自發性高血壓大鼠心肌細胞凋亡的影響。方法 將自發性高血壓大鼠隨機分為治療組[阿折地平3mg/(kg·d)溶于生理鹽水中灌胃]、SHR組，另選WKY大鼠為正常對照組，每組10只。分別每周測大鼠血壓，10周后處死測其左心室肥厚指數，計數細胞凋亡數，ELISA法檢測心肌細胞TNF-α的表達。結果 SHR組與WKY組血壓無明顯改變，治療前后比較亦無明顯差異(P>0.05)，治療組的血壓較治療前顯著降低(P<0.01)，與WKY組比較無明顯差異，治療組左心室肥厚指數、細胞凋亡指數及TNF-α的表達顯著小于SHR組(P<0.01)，治療組與WKY組比較無顯著性差異(P>0.05)。結論 阿折地平能有效的控制血壓，能有效的抑制高血壓所致的左心室肥厚、細胞凋亡及心肌TNF-α的表達。

[關鍵詞] 阿折地平;細胞凋亡;大鼠

[中圖分類號] R972[文獻標識碼] A [文章編號] 1673-9701(2010)04-29-03

Effects of Azelnidipine on Cardiomyocyte Apoptosis of Spontaneously Hypertensive Rats

HU LianfenLIU Guangde

Department of Cardiology，Zhuhai Municipal People’s Hospital，Zhuhai 519000，China

[Abstract] Objective To explore the effect of Azelnidipine on cardiomyocyte apoptosis of spontaneously hypertensive rats(SHR). Methods Twenty SHRs were randomly divided into 2 groups(treatment group and SHR group，with 10 rats in each) and treated with Azelnidipine for 10 weeks and control group(10 WKY rats) was treated with 0.9% sodium chloride solution.The rat blood pressure was measured every week. The rats were sacrificed after 10 weeks，the left ventricular hypertrophy index was measured，the number of cell apoptosis was counted，and the expression of myocardial cell TNF-αwas detected by ELISA. Results Compared with the control group，Azelnidipine could significantly reduce the 1eft ventricular hypertrophic index，cardiomyocyte apoptosis and TNF-α(P<0.01). Conclusion Azelnidipine can effectively control blood pressure，which will inhibit hypertension-induced left ventricular hypertrophy，apoptosis，and myocardial expression of TNF-α.

[Key words] Azelnidipine;Apoptosis;Rat

原發性高血壓病(primary hypertension)是嚴重危害我國人民健康最常見的心血管疾病之一。長期高血壓可導致以左心室肥厚(left ventricular hypertrophy，LVH)為主要特征的心臟病變;WHO已將逆轉LVH及心功能異常、保護靶器官作為治療高血壓病的主要目標之一，因此LVH的逆轉成為高血壓研究領域的新熱點。阿折地平是一種新型二氫吡啶類鈣拮抗藥，國外學者研究表明阿折地平對自發性高血壓大鼠(spontaneous hypertension rat，SHR)的腎臟和心肌缺血的狗具有明顯的器官保護作用[1，2]。這種作用是否通過削弱高血壓發展過程中的心肌細胞凋亡，以及TNF的表達而實現，本實驗就其具體機制進一步研究，為阿折地平在我國引進后的大規模臨床應用提供試驗依據。

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