慢性牙周炎患者齦下菌斑宏轉(zhuǎn)錄組學(xué)分析

肖煒卓 張龍 王曉冬 張保榮

[摘要] 目的 分析慢性牙周炎齦下菌斑的轉(zhuǎn)錄組，探究齦下菌斑群落在慢性牙周炎發(fā)病過稱中的變化。 方法 選取2017年1月～2018年1月在航空總醫(yī)院口腔科診斷為慢性牙周炎并滿足納入條件的7例的齦下菌斑患者，提取相關(guān)RNA，進行微量RNA建庫。應(yīng)用高通量二代測序技術(shù)，對其宏轉(zhuǎn)錄組進行分析，觀察相關(guān)菌群基因表達情況。 結(jié)果 慢性牙周炎齦下菌斑中，文氏密螺旋體的基因序列高度表達。與牙周炎發(fā)生關(guān)系密切的同源蛋白質(zhì)家族中，最多的表達基因序列與細(xì)菌的翻譯相關(guān)。而碳水化合物活性酶相關(guān)的基因表達中，表達最多的是碳水化合物相關(guān)結(jié)構(gòu)域基因序列和糖苷水解酶基因序列。 結(jié)論 通過宏轉(zhuǎn)錄組的測序分析，了解慢性牙周患者齦下菌斑中不同細(xì)菌基因表達情況及相關(guān)細(xì)菌生理代謝的基因表達，從而對牙菌斑的致病性提出新的研究方法和意見，加深對菌斑致病性的理解。

[關(guān)鍵詞] 慢性牙周炎;齦下菌斑;轉(zhuǎn)錄組;高通量測序

[中圖分類號] R781? ? ? ? ? [文獻標(biāo)識碼] A? ? ? ? ? [文章編號] 1673-7210（2019）07（c）-0021-04

Macrotranscriptome analysis of subgingival plaque in patients with chronic periodontitis

XIAO Weizhuo1*? ?ZHANG Long2*? ?WANG Xiaodong3? ?ZHANG Baorong1，3

1.School of Stomatology， Weifang Medical University， Shandong Province， Weifang? ?261000， China; 2.Department of Stomatology， University of Chinese Academy of Sciences， Shenzhen Hospital， Guangdong Province， Shenzhen? ?518000， China; 3.Department of Stomatology， Aviation General Hospital， Beijing? ?100012， China

[Abstract] Objective To analyze the transcriptome of subgingival plaque in chronic periodontitis and explore the changes of subgingival plaque community in chronic periodontitis. Methods From January 2017 to January 2018， 7 cases of subgingival plaque patients diagnosed as chronic periodontitis in the Department of Stomatology of Aviation General Hospital and meeting the inclusion conditions were selected. Relevant RNA was extracted and trace RNA was used to build a database. High-throughput second-generation sequencing technology was used to analyze the macrotranscriptome and the gene expression of related bacteria was observed. Results In the subgingival plaque of chronic periodontitis， the gene sequence of Venn treponema was highly expressed. Among the homologous protein families closely related to periodontitis， the most expressed gene sequences were related to bacterial translation. Among the active carbohydrate enzyme-related genes， the most expressed ones were the sequences of carbohydrate related domain genes and glycoside hydrolytic enzyme genes. Conclusion Macrotranscriptome sequencing analysis is conducted to understand the gene expression of different bacteria in subgingival plaque of chronic periodontitis patients and the gene expression of related bacteria physiological metabolism， so as to propose new research methods and opinions on the pathogenicity of dental plaque， and deepen the understanding of the pathogenicity of plaque.

[Key words] Chronic periodontitis; Subgingival plaque; Transcriptome; High-throughput sequencing

牙周炎是由牙菌斑及其代謝產(chǎn)物引起牙周支持組織喪失的慢性炎癥疾病，其發(fā)病率在80%～90%[1-2]。牙周炎的發(fā)生、發(fā)展與多種因素相關(guān)[3-5]，牙周組織破壞是由牙齦下生物膜中的致病性微生物群引起的[6-7]。

牙周炎發(fā)生發(fā)展過程中的大部分微生物很難培養(yǎng)或不能培養(yǎng)[8-9]，導(dǎo)致其基因表達分析還不夠完善。因此，探究牙周炎病因的關(guān)鍵是對牙菌斑及其代謝產(chǎn)物在發(fā)病過程中的變化[10-11]。……