多因素處理誘導卷丹珠芽發芽和生根研究

摘要 為獲得卷丹百合珠芽快繁的方法，探究多因素處理對卷丹珠芽發芽和生根的影響，利用多因素方差分析低溫預處理、培養基質和培養溫度以及三者之間的互作對卷丹珠芽發芽和生根的影響。結果表明，低溫預處理與培養基質之間的互作對卷丹珠芽發芽的影響表現為極顯著，對珠芽生根的影響表現為顯著，且影響占比最大；培養溫度對珠芽發芽和生根的影響分別表現為極顯著和顯著；培養基質僅對珠芽發芽的影響表現為顯著；將珠芽經4 ℃處理14 d后置于26 ℃發酵鋸末中培養，是誘導卷丹珠芽發芽和生根的最佳方式，培養56 d時發芽率可達98.33%，生根率可達98.89%。研究結果闡明了不同培養因素及其互作對卷丹珠芽繁殖的重要性，為卷丹珠芽的離體快繁提供了技術支持，也為珠芽在其他植物生產栽培過程中的應用提供參考。

關鍵詞 卷丹珠芽；低溫預處理；培養基質；多因素方差分析；發芽率

中圖分類號 S682.2+9 文獻標識碼 A 文章編號 0517-6611（2024）21-0038-06

doi：10.3969/j.issn.0517-6611.2024.21.008

開放科學（資源服務）標識碼（OSID）：

The Induction Method of the Bulbil Germination and Rooting by Multifactor Treatment in Lilium lancifolium

LIU Xuan1，2，MA Yan-ling2，ZHANG Xin-jie2 et al

（1.College of Biology and Food Engineering，Chongqing Three Gorges University，Chongqing 404020;2.School of Advanced Agriculture and Bioengineering，Yangtze Normal University，Chongqing 408100）

Abstract To obtain the method of bulbils rapid propagation in Lilium lancifolium， and explore the effects of multi-factor treatment on germination and rooting of Lilium lancifolium bulbils， the effects of cold temperature pretreatment， culture substrate and temperature， as well as the interaction among the three factors on the germination and rooting efficiency of the bulbils were studied by using multiple factor analysis of variance. The results showed that the interaction between cold temperature pretreatment and culture substrate significantly impacted the germination and rooting efficiency of bulbils， the influence on the germination of bulbils was extremely significant， and the influence on the rooting of bulbils was significant. The influence of culture temperature on the germination and rooting of bulbils was extremely significant and significant respectively;the influence of culture substrate on the germination of bulbils was significant. The optimal method of the bulbil reproduction was that bulbils were treated at cold temperature for 14 days and cultured in fermented sawdust at 26 ℃， which germination rate could reach 98.33% and rooting rate could reach 98.89% after 56 days of culture. These results clarified the importance of different culture factors and their interactions on the propagation of bulbils of L.lancifolium， provided technical support for the rapid propagation of bulbils of L.lancifolium in vitro， and also provided reference for the application of bulbils in the production and cultivation of other plants.

Key words Lilium lancifolium bulbils;Low-temperature pretreatment;Culture substrate;Multivariate analysis of variance;Germination efficiency

基金項目 重慶市涪陵區科技局技術創新與應用發展項目（FLKJ，2023AAN1001）；重慶市科衛聯合中醫藥技術創新與應用發展項目（2020ZY3907）；重慶市科學技術局績效激勵引導專項（cstc2021-jxjl130019）；長江師范學院人才引進項目（2017KYQD63）。

作者簡介 劉萱（1997—），女，四川南充人，碩士研究生，研究方向：百合栽培與基因功能分析。*通信作者：雷美艷，副研究員，碩士，從事中草藥栽培與開發利用研究；符勇耀，副教授，博士，從事百合育種與抗逆分子機理研究。……