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關鍵詞 柳州異裂菊;凹脈異裂菊;正交試驗;扦插;成活率;葉綠素含量
中圖分類號 S604+.3 文獻標識碼 A 文章編號 0517-6611(2024)21-0030-08
doi:10.3969/j.issn.0517-6611.2024.21.007
開放科學(資源服務)標識碼(OSID):
Research on Cutting Technology of Endangered Plants Heteroplexis incana and Heteroplexis impressinervia
BAO Su-qing1,2, DENG Li-li2, WEI Xiao2 et al
(1.Guilin Medical University, Guilin, Guangxi 541199;2.Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Scences, Guilin, Guangxi 541006)
Abstract [Objective] To screen the optimal combination of H.incana and H.impressinervia for cutting propagation in Liuzhou, a rare and endangered plant, and to lay a foundation for its germplasm conservation and development. [Method] The healthy cuttings were selected and the orthogonal design was used to investigate the effects of four factors, including cutting size, substrate type, plant growth regulator type and concentration, on the growth characteristics of the cuttings, and the optimal combination was selected. [Result] The survival rate of 9 combinations was significantly different, and the survival rate of combination ③ was the highest (40%). There were no significant differences in root length and root diameter of 9 combinations (P>0.05), but there were significant differences in root number of 9 combinations (P<0.05). Combination ③ was beneficial to root growth. The survival rate of H.incana group ① was the highest, reaching 20%. There were no significant differences in the number of branches, height of branches, thickness of branches, number of leaves and length of leaves among the 9 groups (P>0.05), but there were significant differences in leaf width (P<0.05). [Conclusion] The cuttings of the 6.0-10.0 mm coarse panicle soaked with 150 mg/L NAA in the soil matrix are beneficial to the survival of the cuttings and the growth of the seedlings, while the same coarse panicle soaked with 50 mg/L SGJ in the loess matrix is better.
Key words Heteroplexis incana;Heteroplexis impressinervia;Orthogonal test;Cutting technology;Survival rate;Chlorophyll content
基金項目 國家自然科學基金項目(31960276)。
作者簡介 包素青(1998—),女,廣西鳳山人,碩士研究生,研究方向:藥物研發與轉化。
*通信作者,研究員,從事藥用植物及保育生物學研究。
收稿日期 2023-11-15;修回日期 2023-12-18
柳州異裂菊(Heteroplexis incana J.Y.Liang)和凹脈異裂菊(Heteroplexis impressinervia J.Y.Liang)為廣西喀斯特地區特有屬異裂菊屬(Heteroplexis)植物,多分布于廣西陽朔、龍州、融安等地石灰巖石山的中上部,分布區域極為狹窄[1],這2種植物對于菊科系統演化等研究具有重要的科學意義,而民間常用于治療消化不良、脾虛、浮腫和小便不利等病癥[2]。……