MiR-206、TGF-β1和Smad3在妊娠期高血壓疾病患者胎盤組織中的表達(dá)及意義

［摘 要］目的 探討妊娠期高血壓疾病患者胎盤組織微小RNA-206（miR-206）、轉(zhuǎn)化生長因子β1（TGF-β1）、Sma和Mad相關(guān)蛋白3（Smad3）的表達(dá)情況及臨床意義。方法 選取2020年6月至2023年1月于河北中石油中心醫(yī)院就診的60例妊娠期高血壓患者（妊娠期高血壓組）、60例子癇前期患者（子癇前期組）、60例妊娠晚期健康孕婦（對照組）為研究對象。采用實(shí)時熒光定量PCR（qRT-PCR）法檢測miR-206、TGF-β1和Smad3的mRNA水平，采用免疫組化法檢測胎盤組織中TGF-β1和Smad3蛋白表達(dá)；采用Pearson相關(guān)分析妊娠期高血壓疾病患者胎盤組織miR-206、TGF-β1 mRNA和Smad3 mRNA水平與臨床資料的相關(guān)性；采用受試者工作特征（ROC）曲線分析產(chǎn)后胎盤組織miR-206、TGF-β1 mRNA和Smad3 mRNA水平對妊娠期高血壓疾病孕婦病情的評估價值。結(jié)果 對照組、妊娠期高血壓組、子癇前期組的舒張壓、收縮壓、24h尿蛋白、TGF-β1 mRNA、Smad3 mRNA、TGF-β1蛋白表達(dá)陽性率、Smad3蛋白表達(dá)陽性率均依次升高，清蛋白、新生兒體重、胎盤重量、miR-206均依次降低，差異有統(tǒng)計學(xué)意義（F/χ2值介于39.400～834.610之間，P＜0.05）；妊娠期高血壓疾病患者胎盤組織miR-206與收縮壓、舒張壓、24h尿蛋白負(fù)相關(guān)，與清蛋白、新生兒體重、胎盤重量正相關(guān)（r值介于－0.514～0.507之間，P＜0.05）；TGF-β1 mRNA、Smad3 mRNA與清蛋白、新生兒體重、胎盤重量負(fù)相關(guān)，與收縮壓、舒張壓、24 h尿蛋白正相關(guān)（r值介于－0.512～0.519之間，P＜0.05）；胎盤組織miR-206、TGF-β1 mRNA和Smad3 mRNA水平單獨(dú)及聯(lián)合評估妊娠期高血壓疾病孕婦病情的曲線下面積（AUC）分別為0.781、0.840、0.837、0.951。結(jié)論 miR-206在妊娠期高血壓疾病患者胎盤組織中低表達(dá)，TGF-β1和Smad3高表達(dá)，三者可能共同參與了妊娠期高血壓疾病進(jìn)展。

［關(guān)鍵詞］妊娠期高血壓疾病；胎盤組織；微小RNA-206；轉(zhuǎn)化生長因子β1；Sma和Mad相關(guān)蛋白3

Doi：10.3969/j.issn.1673-5293.2024.09.003

［中圖分類號］R173""" ［文獻(xiàn)標(biāo)識碼］A

［文章編號］1673-5293（2024）09-0014-07

Expression and significance of miR-206，TGF-β1 and Smad3 in placental tissue of

patients with hypertensive disorders of pregnancy

［Abstract］ Objective To explore the expression and clinical significance of microRNA-206 （miR-206），transforming growth factor-β1 （TGF-β1） and Sma-and Mad-related protein 3 （Smad3） in placental tissue of patients with hypertensive disorders of pregnancy. Methods A total of 60 patients with gestational hypertension （gestational hypertension group），60 patients with preeclampsia （preeclampsia group），and 60 healthy pregnant women in late pregnancy （control group） who were treated at Hebei Petro China Central Hospital from June 2020 to January 2023 were selected as the study subjects.Real-time fluorescence quantitative PCR （qRT-PCR） was used to detect the mRNA levels of miR-206，TGF-β1 and Smad3，and immunohistochemistry was used to detect the protein expressions of TGF-β1 and Smad3 in placental tissues.Pearson correlation was applied to analysis the correlation between the levels of miR-206，TGF-β1 mRNA and Smad3 mRNA levels in placental tissues and clinical data in patients with hypertensive disorders of pregnancy.Receiver operating characteristic （ROC） curves were used to analyze the value of postpartum placental tissue miR-206，TGF-β1 mRNA and Smad3 mRNA levels in assessing the condition of pregnant women with hypertensive disorders of pregnancy. Results The diastolic blood pressure，systolic blood pressure，24-hour urine protein，TGF-β1 mRNA，Smad3 mRNA，positive expression rate of TGF-β1 protein，and positive expression rate of Smad3 protein in the control group，gestational hypertension group，and preeclampsia group increased in turn，while albumin，neonatal weight，placental weight，and miR-206 decreased sequentially，with significant differences （F/χ2=39.400-834.610，P＜0.05）.In patients with gestational hypertension，miR-206 in placental tissue was negatively correlated with systolic blood pressure，diastolic blood pressure，and 24 h urine protein，and positively correlated with albumin，neonatal weight，and placental weight （r=－0.514-0.507，P＜0.05）.TGF-β1 mRNA and Smad3 mRNA were negatively correlated with albumin，neonatal weight and placental weight，and were positively correlated with systolic blood pressure，diastolic blood pressure，and 24 h urine protein （r =－0.512-0.519，P＜0.05）.The AUC of miR-206，TGF-β1 mRNA and Smad3 mRNA levels in placental tissue for alone and in combination evaluating the condition of pregnant women with hypertensive disorders were 0.781，0.840，0.837 and 0.951，respectively. Conclusion MiR-206 is lowly expressed in placental tissues of patients with hypertensive disorders，while TGF-β1 and Smad3 are highly expressed.The three may be involved in the progression of hypertensive disorders.

［Key words］ hypertensive disorders of pregnancy;placental tissues;micro RNA-206;transforming growth factor β1;Sma-and Mad-related protein 3

妊娠期高血壓疾病是妊娠后期常見的一種妊娠期并發(fā)癥，其發(fā)病機(jī)制尚未明確［1］。……