大葉補血草根莖總黃酮的提取和抗氧化活性研究

陳梅 葛鵬 何春霞

摘要 [目的]研究大葉補血草根莖總黃酮提取物的抗氧化活性。[方法]采用超聲提取考察不同產地不同溶劑提取的大葉補血草總黃酮含量;采用Fenton反應體系、改良的鄰苯三酚自氧化體系和硫代巴比妥酸（TBA）法分別測定大葉補血草總黃酮對羥自由基、超氧陰離子自由基和脂質過氧化的抑制作用。[結果]醇提總黃酮的產率高于水提取，產于阿勒泰的大葉補血草總黃酮含量略高;抗氧化試驗結果顯示，大葉補血草總黃酮對·OH、 O2·-的產生和脂質過氧化均具有抑制作用，采于阿勒泰地區的大葉補血草中總黃酮的抗氧化活性略高于伊犁地區的，而醇提取液的抗氧化活性明顯大于水提液的活性，當濃度達到0.83 mg/mL時，醇提取液對羥自由基（·OH）的清除率達98.9%。[結論]大葉補血草根部總黃酮含量可觀，作為抗氧化劑具有很好的開發應用價值和前景。

關鍵詞 大葉補血草;根莖;總黃酮;提取;抗氧化活性

中圖分類號 R282.71文獻標識碼 A

文章編號 0517-6611（2019）19-0190-03

doi：10.3969/j.issn.0517-6611.2019.19.055

Abstract [Objective] The research aimed to study the antioxidant activity of the total flavonoid extract from the rhizome of Limonium gmelinii.[Method]The content of total flavonoids in Limonium gmelinii from different areas was investigated by ultrasonic extraction with different solvents. Fenton reaction，improved pyrogallol autoxidation system and TBA method were used to measure the inhibition effect of ·OH，O2·- and lipid peroxidation by total flavonoids.[Result]The yield of total flavonoids from alcohol extraction was higher than that from water extraction， and the content of total flavonoids from ALTGL was slightly higher; the results of antioxidation experiments showed that the total flavonoids possessed certain inhibition effect of ·OH，O2·- and lipid peroxidation，the antioxidant activity of ALTGL slightly higher than KTGL， and that of alcohol extract was significantly greater than water extract， the clearance rate of hydroxyl free radical by alcohol extract was reached 98.9% when the concentration of 0.83 mg/mL. [Conclusion]The total flavonoids content of Limonium gmelinii root is considerable， and it has great development application value and prospect as an antioxidant.

Key words Limonium gmelinii （Wiid）Kuntze;Rhizome;Total flavonoids;Extract;Antioxidant activity

大葉補血草（Limonium gmelinii（Wiid）Kuntze）系白花丹科補血草屬多年生草本耐鹽旱中生植物，主要分布于新疆的阿勒泰、伊犁等地區，其根莖是新疆維吾爾族和哈薩克族的傳統用藥，主要用于婦科疾病，比如宮頸糜爛、子宮內膜炎等炎癥的治療以及子宮出血、尿血等病癥的止血治療。王仁[1]的《哈薩克藥志》記載了大葉補血草在民間用于治療婦科疾病的多種附方，全草的黃酮類化合物含量豐富。后續的研究也證明了該草富含黃酮物質，2006年，哈薩克斯坦阿爾·法拉比國立大學的Zhusupova教授的課題小組從大葉補血草中純化分離得到了槲皮素、蘆丁、楊梅素及其糖苷類等黃酮類化合物[2]?！?br>