轉錄因子SOX7對前列腺癌PC-3細胞生物學活性的影響及其機制

鄭斌，吳齊全，周克文，王鋼，任雨，祁洪剛，朱偉智，翁國斌

（寧波市鄞州第二醫(yī)院 泌尿外科，浙江 寧波 315100）

轉錄因子SOX7對前列腺癌PC-3細胞生物學活性的影響及其機制

鄭斌，吳齊全，周克文，王鋼，任雨，祁洪剛，朱偉智，翁國斌

（寧波市鄞州第二醫(yī)院 泌尿外科，浙江 寧波 315100）

目的：探討轉錄因子SOX7對前列腺癌PC-3細胞增殖、細胞周期、遷移和侵襲能力的影響及其機制。方法：采用脂質體法轉染高表達SOX7重組質粒（pCDNA3.1-SOX7），以轉染空質粒（Vector）作為對照，檢測SOX7對PC-3細胞株增殖、細胞周期、遷移及侵襲的影響，并用Western blot法檢測相關蛋白表達水平。結果：pCDNA3.1-SOX7顯著抑制PC-3細胞增殖能力，并且誘導細胞周期發(fā)生G1期阻滯，同時，使Cylcin D1、E表達量明顯下調。此外，pCDNA3.1-SOX7轉染后，PC-3細胞遷移距離顯著減少，穿透基質膠的細胞數量也顯著降低，并且伴隨著間質表型標記物MMP-2、MMP-9和N-cadherin蛋白表達減少，上皮標記物E-cadherin蛋白表達增加。結論：SOX7抑制前列腺癌PC-3細胞的增殖、周期、遷移及侵襲能力，其機制可能與調控這些細胞行為的相關蛋白表達有關。

SOX7；前列腺癌；增殖；遷移；侵襲

Abstract: Objective:To investigate the effects and mechanisms of transcription factor SOX7 on PC-3 cells proliferation, cell cycle, cell migration and invasion.Methods:PC-3 cells were transfected with highexpressed SOX7 recombinant plasmid (pCDNA3.1-SOX7) and further detected the changes of cell proliferation,cell cycle, migration, invasion, and related protein expressions.Results:After transfection with pCDNA3.1-SOX7, significant inhibition effects was shown in the PC-3 cell proliferation and G1/S cell cycle arrest, which were accompanied with decreased expressions of cyclin D1 and E. Furthermore, transfection of pCDNA3.1-SOX7 markedly decreased the cell migration distances and invasion capacity, with a reduction of the mesenchymal markers (MMP-2, MMP-9 and N-cadherin), however, the epithelial marker E-cadherin was increased.Conclusion:SOX7 inhibits PC-3 cell proliferation, cell cycle, migration and invasion through regulation of its related protein expressions.

Key words:SOX7; prostate cancer; proliferation; migration; invasion

前列腺癌為西方男性人群最常見的惡性腫瘤之一，近年來在我國的發(fā)病率與病死率呈逐年上升趨勢[1-2]。臨床上一般采取手術切除和放射療法為主，然而腫瘤復發(fā)與轉移問題已成為目前治療的難點。上皮間質轉化（epithelial-mesenchymal transition，EMT）過程是腫瘤侵襲和轉移的關鍵環(huán)節(jié)。在此過程中，上皮細胞表型轉化為成纖維細胞的間質表型，上皮細胞間緊密連接相關蛋白表達下調，細胞外基質增多，抗凋亡能力增強，從而促進細胞遷移與侵襲[3]。……