柳鳳 歐雄常 詹儒林 韋繼光 常金梅
摘 要 為探討杧果感染畸形病過程中頂芽內酚類代謝的變化規律,以凱特杧為試驗材料,測定了接種病菌(Fusarium mangiferae)后頂芽內總酚、類黃酮含量及酚類代謝相關酶(POD、PPO和PAL)活性的變化趨勢。結果表明,接種病菌后杧果頂芽內總酚、類黃酮含量出現先上升后下降的變化趨勢,其峰值分別出現在接種病菌45 d和30 d,酚類代謝相關酶中PAL活性變化與總酚含量變化趨勢相同,POD和PPO活性在接種病菌后直線上升。F. mangiferae侵染對杧果酚類代謝產生顯著影響,可干擾杧果頂芽內總酚、類黃酮的含量變化,影響其相關酶活性,降低杧果對病菌的抵抗能力。杧果酚代謝紊亂失衡可能是病菌的重要致病機理之一。
關鍵詞 杧果畸形病;Fusarium mangiferae;侵染;酚類代謝
中圖分類號 S667.7 文獻標識碼 A
Effects of Fusarium mangiferae on Phenolic Metabolism in Mango
LIU Feng1,2, OU Xiongchang1, ZHAN Rulin1, WEI Jiguang2, CHANG Jinmei1
1 South Asia Tropical Crop Research Institute, CATAS, Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture,
Zhanjiang, Guangdong 524091, China
2 Agricultural Colleges, Guangxi University, Nanning, Guangxi 530004, China
Abstract In order to study the effect of pathogens causing malformation on phenolic metabolism in mango, we detected the changes of phenolics and flavonoids contents, peroxidase(POD), phenylalanine ammonia-lyase(PAL)and polyphenol oxidase(PPO)activities in mango infected by Fusarium mangiferae. The results showed that, total contents of phenolics and flavonoids and activity of phenylalanine ammonia-lyase(PAL)were significantly increased in 45 days after inoculation,then decreased quickly, the activity of polyphenol oxidase(PPO)and peroxidase(POD)did not change obviously at the beginning after inoculation, then increased continuously. The pathogens could affect the phenolic metabolism in mango significantly, which related to its resistance reducing. The phenolic metabolism disturbances may be one of the important reasons about the disease occurs.
Key words Mango malformation; Fusarium mangiferae; Infection; Phenolic metabolism
doi 10.3969/j.issn.1000-2561.2015.01.025
酚類是植物抗病反應的主要生化物質,是一類苯環上含有一個或多個羥基的芳香族衍生物,能夠被逆境的生物和非生物因子誘導合成[1-2]。植物體內的酚類物質代謝網是一個錯綜復雜的過程,酚類物質可轉化為醌類和木質素等其他抗性更強的物質,間接提高植物的抗病性[3-4]。許多研究結果表明,酚類代謝在植物與病原生物互作過程中參與許多生理過程,如木質化形成、氧化還原反應、對毒素活性的反應和刺激反應等[5-6]。杧果畸形病是一種危害杧果正常生長的世界性病害,植株感病后嫩葉變細而脆,嫩芽、花序簇生,最后干枯,因此杧果染病后幾乎不座果。國內該病主要分布在云南、四川海拔較高的杧果晚熟地區[7-8]。……