基于殼聚糖雙胍鹽的復合薄膜制備及其性能分析

摘 要： 為了阻止食品微生物污染和延長食品保質期，以殼聚糖雙胍鹽（Chitosan biguanidine hydrochloride，CSG）為主體原料，將其與普魯蘭多糖（Pullulan， PLL）和聚乙烯醇（Polyvinyl alcohol，PVA）混合后經溶液澆注法制備復合薄膜。分析制備所得復合薄膜的形貌和結構，探究復合薄膜的水汽屏障性、水穩定性、力學性能、熱穩定性、抗菌性能以及生物相容性等性能。結果表明：通過氫鍵相互作用形成的復合薄膜內部結構均勻且緊密；PLL和PVA的引入不僅增強了復合薄膜的透光率和力學性能，還能提高其水蒸氣阻隔性和熱穩定性；復合薄膜表現出顯著的抗菌效果和良好的生物相容性，對金黃色葡萄球菌和大腸埃希菌的抑菌率可達99.90%。該復合薄膜在阻止微生物污染和延長食品保質期的包裝薄膜制備中具有良好的應用前景。

關鍵詞：殼聚糖雙胍鹽；普魯蘭多糖；聚乙烯醇；包裝薄膜；抗菌

中圖分類號：TB324

文獻標志碼：A

文章編號：1673-3851 （2025） 03-0185-13

引文格式：何智敏，時均浩，楊雅靜，等. 基于殼聚糖雙胍鹽的復合薄膜制備及其性能分析［J］. 浙江理工大學學報（自然科學），2025，53（2）：185-197.

Reference Format： HE Zhimin，SHI Junhao，YANG Yajing，et al. Preparation and performance analysis of a composite film based on chitosan biguanidine hydrochloride［J］. Journal of Zhejiang Sci-Tech University，2025，53（2）：185-197.

Preparation and performance analysis of a composite film based on chitosan biguanidine hydrochloride

HE Zhimin¹， SHI Junhao¹， YANG Yajing¹， YANG Hanyu¹， LUAN Liang²， ZHANG Quan¹

（1.School of Materials Science amp; Engineering， Zhejiang Sci-Tech University， Hangzhou 310018， China;

2.Department of Laboratory Medical Center， General Hospital of Northern Theater Command， Shenyang 110016， China）

Abstract：To prevent microbial contamination and improve food preservation， a composite film was developed by blending chitosan biguanidine hydrochloride （CSG） with pullulan （PLL） and polyvinyl alcohol （PVA） using the solution casting method. The morphology and structure of the prepared composite film were analyzed， and various properties including water vapor barrier， water stability， mechanical properties， thermal stability， antibacterial efficacy， and biocompatibility were investigated. The results showed that the composite film， formed through hydrogen bonding interactions， possessed a cohesive and dense structure. Furthermore， the incorporation of PLL and PVA not only enhanced transparency and mechanical robustness of the film， but also improved water vapor barrier and thermal stability. The composite film exhibited significant antibacterial activity and excellent biocompatibility， achieving antibacterial rates of 99.90% against Staphylococcus aureus and Escherichia coli. This composite film shows great potential for packaging applications aimed at safeguarding food products against microbial contamination and extending their shelf-life.

Key words：chitosan biguanidine hydrochloride; pullulan; polyvinyl alcohol; packaging film; antibacterial

0 引 言

據統計，每年有超過三分之一的果蔬在食用前就發生了腐爛變質，導致嚴重的食物浪費和經濟損失^［1-2］。果蔬在運輸、儲存過程中的水分流失和微生物滋生會顯著影響果蔬的新鮮度^［3］，使得果蔬腐爛變質。目前商業上常采用打蠟^［4］、冷藏^［5］和氣調包裝^［6］等方式來延長果蔬的儲存時間。然而，打蠟的水果表面蠟殘留難以除凈，會對人體健康產生危害。制冷保鮮和氣調包裝保鮮方式需要專用設備，能源消耗量大，成本高^［7］。……