黑果枸杞SPL轉錄因子家族成員鑒定與生物信息學分析

摘要： SPL（SQUAMOSA promoter-binding protein-like）家族成員參與植物的生長發育和脅迫響應，為探究黑果枸杞SPL家族基因在鹽脅迫下的耐鹽分子機制，本研究對其基因家族成員進行了鑒定和生物信息學分析。結果表明，從黑果枸杞轉錄組數據中共篩選出20個SPL基因家族成員，分為8個亞家族，蛋白質亞細胞定位預測結果顯示SPL蛋白主要定位于細胞核，20個SPL蛋白均具有SBP保守結構域。黑果枸杞SPL家族蛋白質相對分子量為15 296.08～130 310.8；等電點為5.64～9.65；總原子數為2 106～18 214；氨基酸數量為133～1 176 aa；脂溶指數為46.35～87.53；均為親水性蛋白質和不穩定蛋白質。黑果枸杞SPL家族蛋白質結構分析結果表明，無規則卷曲和α-螺旋占比較大。表達模式分析結果表明，在NaCl脅迫下不同器官中黑果枸杞SPL家族基因具有不同的表達趨勢，LrSPL9在根和葉中均為高表達。該研究結果可為黑果枸杞SPL基因家族成員鹽脅迫響應方面的進一步研究提供參考。

關鍵詞： 黑果枸杞；NaCl脅迫；SPL轉錄因子；生物信息學分析

中圖分類號： S722.3⁺6 文獻標識碼： A 文章編號： 1000-4440（2024）11-2001-12

Identification and bioinformatics analysis of SPL transcription factor family members in Lycium ruthenicum

LIU Yun¹， GUO Yi²， LI Xiaoxiong³， HU Xiaotong¹， MA Rui¹， DU Yu¹， JIA Xibei¹， LIU Di¹， MA Yanjun¹

（1.College of Forestry， Gansu Agricultural University， Lanzhou 730070， China;2.Gansu Forestry Polytechnic， Tianshui 741020， China;3.Lanzhou Resources amp; Environment Voc-Tech University， Lanzhou 730030， China）

Abstract： The SQUAMOSA promoter-binding protein-like （SPL） family members are involved in plant growth and development and stress response. To investigate the salt tolerance molecular mechanism of Lycium ruthenicum SPL family genes under salt stress， the gene family members were identified and analyzed by bioinformatics. The results showed that 20 SPL family members were screened from transcriptome data of Lycium ruthenicum， and they were divided into eight subfamilies. The results of protein subcellular localization prediction showed that SPL proteins were mainly located in the nucleus， and 20 SPL proteins had SBP conserved domains. The relative molecular weight of SPL family proteins in Lycium ruthenicum was 15 296.08-130 310.8. The isoelectric point was 5.64-9.65， the total number of atoms was 2 106-18 214， the number of amino acids was 133-1 176 aa， and the lipid solubility index was 46.35-87.53. All of them were hydrophilic proteins and unstable proteins. The results of protein structure analysis of SPL family in Lycium ruthenicum showed that random coil and α-helix accounted for a large proportion. The results of expression pattern analysis showed that the SPL family genes of Lycium ruthenicum had different expression trends in different organs under NaCl stress， and LrSPL9 was highly expressed in roots and leaves. The results of this study can provide a reference for further research on salt stress response of SPL gene family members in Lycium ruthenicum.

Key words： Lycium ruthenicum;NaCl stress;SPL transcription factor;bioinformatics analysis

SPL（SQUAMOSA promoter-binding protein-like）是一類廣泛存在于植物中的重要的特異性轉錄因子，其蛋白質均具有SBP結構域^[1-2]。SPL轉錄因子最初是在金魚草（Antirrhinum majus L.）中發現并由此得名^[3]，SPL在植物生長發育過程中參與花的發育及調控、根的發育、非生物與生物的脅迫應答^[4-5]。例如在鹽脅迫或干旱脅迫下SPL家族成員會通過調節基因參與信號轉導的豐度、脯氨酸合成和花青素代謝等來響應鹽脅迫或干旱脅迫^[2]。研究人員在對擬南芥（Arabidopsis thaliana）的研究中發現，沉默miR156靶向的SPL2、SPL9、SPL11基因，可增強擬南芥遭受高溫脅迫、鹽脅迫和干旱脅迫后的恢復能力^[6]；……