OLR1基因生信分析及其在貴州黑山羊和黔北麻羊性腺軸中的表達(dá)

摘要 ［目的］研究OLR1基因與貴州黑山羊和黔北麻羊繁殖率之間的關(guān)系，為研究OLR1基因調(diào)控山羊卵泡發(fā)育的分子機(jī)制提供參考。［方法］通過(guò)生物信息學(xué)的手段對(duì)OLR1蛋白理化性質(zhì)、亞細(xì)胞定位、信號(hào)肽位點(diǎn)、蛋白高級(jí)結(jié)構(gòu)等進(jìn)行預(yù)測(cè)，并通過(guò)對(duì)不同物種OLR1氨基酸序列進(jìn)行同源性分析，構(gòu)建系統(tǒng)發(fā)育進(jìn)化樹(shù)；采用RT-qPCR 的方法檢測(cè)OLR1基因在單羔和多羔貴州黑山羊和黔北麻羊性腺軸各組織中的表達(dá)量。［結(jié)果］OLR1基因編碼 275個(gè)氨基酸，理論pi值為6.37，OLR1是一種主要由α-螺旋卷曲構(gòu)成的不具有信號(hào)肽的不穩(wěn)定親水性蛋白；與其他動(dòng)物相比，山羊OLR1與反芻動(dòng)物綿羊和牛的遺傳距離最近。qPCR 結(jié)果顯示：OLR1基因在貴州黑山羊及黔北麻羊下丘腦、輸卵管、垂體、子宮和卵巢組織中均有表達(dá)。在單羔組貴州黑山羊下丘腦、子宮、卵巢、垂體中的表達(dá)量均極顯著高于多羔組（P<0.01），在輸卵管中的表達(dá)量高于多羔組，但二者間差異不顯著（P>0.05）。在單羔組黔北麻羊下丘腦、垂體、卵巢中的表達(dá)量均極顯著高于多羔組（P<0.01）；在輸卵管中的表達(dá)量高于多羔組，在子宮中的表達(dá)量低于多羔組，但二者間差異不顯著（P>0.05）。［結(jié)論］OLR1基因表達(dá)量與貴州黑山羊和黔北麻羊的繁殖性狀呈負(fù)相關(guān)，可作為影響貴州本地山羊繁殖性能的重要候選基因。

關(guān)鍵詞 貴州黑山羊；黔北麻羊；性腺軸；OLR1基因；生物信息學(xué)；繁殖性狀

中圖分類(lèi)號(hào) S 826 文獻(xiàn)標(biāo)識(shí)碼 A 文章編號(hào) 0517-6611（2024）22-0088-05

doi：10.3969/j.issn.0517-6611.2024.22.017

開(kāi)放科學(xué)（資源服務(wù)）標(biāo)識(shí)碼（OSID）：

Bioinformatics Analysis of OLR1 Gene and Its Expression in Gonadal Axis of Guizhou Black Goat and Qianbei Ma Goat

YANG Hong1， YANG Yuan-qing1， ZHOU Ming-shuai2 et al

（1. Bijie Livestock Station， Bijie， Guizhou 551700;2. College of Animal Science， Guizhou University/Key Laboratory of Animal Genetics， Breeding and Reproduction of Plateau Mountainous Animals， Ministry of Education， Guiyang， Guizhou 550025）

Abstract ［Objective］To study the relationship between OLR1 gene and the reproductive rate of Guizhou black goat and Qianbei Ma goat， and to provide reference for studying the molecular mechanism of OLR1 gene regulating goat follicular development. ［Method］The physicochemical properties， subcellular localization， signal peptide sites， and advanced structure of OLR1 protein were predicted by bioinformatics methods. The phylogenetic tree was constructed by homology analysis of OLR1 amino acid sequences of different species. Then RT-qPCR was used to detect the expression of OLR1 gene in the tissues of the gonadal axis of single-lamb and multi-lamb Guizhou black goats and Qianbei Ma goats. ［Result］OLR1 gene encoded 275 amino acids， and the theoretical pi value was 6.37. OLR1 was an unstable hydrophilic protein without signal peptide， which was mainly composed of α-helix curl. Compared with other animals， goat OLR1 has the closest genetic distance to ruminant sheep and cattle. The results of qPCR showed that OLR1 gene was expressed in hypothalamus， oviduct， pituitary， uterus and ovary tissues of Guizhou black goat and Qianbei Ma goat. The expression in hypothalamus， uterus， ovary and pituitary of Guizhou black goat in single-lamb group was significantly higher than that in multi-lamb group （P<0.01）. The expression levels in hypothalamus， pituitary and ovary of Qianbei Ma goat in single lamb group were significantly higher than those in multiple lamb group （P<0.01）. ［Conclusion］The expression level of OLR1 gene was negatively correlated with the reproductive traits of Guizhou black goats and Qianbei Ma goats， which could be used as an important candidate gene affecting the reproductive performance of Guizhou local goats.

Key words Guizhou black goat;Qianbei Ma Goat;Gonadal axis;OLR1 gene;Bioinformatics;Reproductive traits

氧化型低密度脂蛋白受體1（OLR1）是一種凝集素清道夫受體，可識(shí)別多種配體，如氧化型低密度脂蛋白、多陰離子化學(xué)物質(zhì)、陰離子磷脂等，具有結(jié)合、內(nèi)化和降解低密度脂蛋白的作用^［1-2］，主要分布于血管內(nèi)皮細(xì)胞和腎、肺、神經(jīng)元等血管豐富的組織中^［1］。……