Effect of nano-selenium loaded with lycium barbarum polysaccharide on the proliferation of lens epithelial cells after UVB damage in vitro

INTRODUCTION

2017年2月—2018年2月，采用方便抽樣方法選取到我院進行診療的首次上消化道出血患者458例。納入標準：確診為上消化道出血，有嘔血和（或）便血癥狀；有認知和行為能力；有溝通和配合能力；住院天數≥3 d；自愿參與者。排除標準：認知障礙或行動不便，無法理解和配合研究者；有嚴重并發癥；患有其他慢性疾病或嚴重腦、心、腎、肝等臟器疾病；不愿參與者。采用隨機數字法分為觀察組與對照組，每組各229例。兩組患者的年齡、性別、病程等一般資料經比較，差異無統計學意義（P＞0.05），具有可比性。

MATERIALS AND METHODS

According to the results, after damaged with UV, SRA01/04 cells were incubated with 2.0 mg/mL LBP-SeNPs and photographed 24h later. The 2.0 mg/mL LBP-SeNPs showed a higher cell density at 0.5 μmol/L. As the concentration increased, cytoplasmic dehydration, cell crumbling and apoptotic vesicles appeared more, SRA01/04 cells density decreased (Figure 8).

The SV40 T-antigen-transformed HLECs,SRA01/04, was obtained from the Cancer Institute of the Chinese Academy of Medical Sciences (Beijing, China). Cells were cultured in DMEM supplemented with 10% FBS, 100 U/mL penicillin, and 100 mg/mL streptomycin and incubated at 37℃in a 5% COincubator.

3D打印是一種快速成形技術，根據所設計的3D模型,通過3D打印設備逐層增加材料來制造三維產品[1-7]。3D打印綜合了機電控制、數字建模、信息、材料科學與化學等諸多領域的前沿技術,被譽為“第三次工業革命”的核心技術[8-11]。由于3D打印具有快速成型、高度靈活、節約成本的優勢,已被廣泛應用于制造業、航空航天、食品產業、建筑及醫療等領域[12]。醫療行業目前是3D打印技術擴張最為迅猛的行業，應用包括醫學模型制造、組織器官再生、肢體修復、骨骼重建等，在個性化解決方案方面，比較典型的應用有3D手術規劃模型、3D打印植入物以及假肢矯形器等康復輔具。

SRA01/04 suspensions were placed in 96-well cell culture plates at a concentration of approximately 5×10cells/mL and grouped according to the UV irradiation time (0, 10, 20, 30, 40, 50,60min), each group was replicated in 6 wells with a volume of 100 μL. After 24h of cell culture, the culture medium was sucked out and 20 μL phosphate buffered saline (PBS) was added to prevent cell apoptosis due to excessive dryness during irradiation. Cells were irradiated in an ultraviolet light box at a wavelength of 302 nm, and one group was uncovered every 10min until the end of 60min. After that, the cells continued to be cultured for 24h. Methylthiazol tetrazolium (MTT) was used to determine the survival rate of the cells.

Oxidative stress induced lens epithelial cell apoptosis plays an important role in cataract formation. It is very important to reduce the apoptosis of lens epithelial cells to control the occurrence and development of cataract. There were some studies on the use of selenium or SeNPs for the treatment of age-related cataracts. Zhuand Lubelieved selenium supplementation could slow the development of naphthalene cataract possibly by attenuating the oxidative stress in the lens. Postfound low serum selenium levels may constitute a potential risk factor of age-related cataract.Based on those, we prepared LBP-SeNPs with suitable particle size and good stability and investigated whether LBP-SeNPs could protect SRA01/04 cells from UV irradiation. Damage model was HLECs (SRA01/04) in logarithmic growth phase with UV irradiation.

According to the above experimental steps, SRA01/04 cells were damaged by ultraviolet radiation for 10min. Different concentrations of LBP-SeNPs were administered immediately after that steps.Cell activity was detected by MTT after 24h incubation. The LBP-SeNPs group with the highest cell proliferation rate was photographed to observe the cell status.

The particle sizes of the prepared LBP-SeNPs were examined using a nanoparticle size analyzer, and the average particle sizes corresponding to the LBP concentrations of 0.5, 1.0, 1.5, and 2.0 mg/mL are shown in Figure 2A, and the particle sizes of both loaded and unloaded LBP-SeNPs were in the range of 150-180 nm, and only the average particle size of 1.5 mg/mL LBP-SeNPs was less than 160 nm. As shown in Figure 2B,the particle size of LBP-SeNPs basically remained between 150-200 nm with time, indicating their stability.

第一，與正常語速相比，較慢語速是否對提高中國不同聽力水平(較低中等水平和較高中等水平)英語學習者的聽力理解能力更有效;第二，與正常語速相比，較慢語速是否對提高中國不同聽力水平(較低中等水平和較高中等水平)英語學習者對短對話、長對話和短文三種不同聽力測試題型的理解能力更有效;第三，語速、英語學習者的聽力水平和不同聽力測試題型之間是否存在交互作用?！?br>