驢源沙門氏菌的分離鑒定、耐藥及毒力分析

孫陽陽　楊美　解曉莉　程凱慧　張亮　楚會萌　任亞初　楊宏軍

摘要：沙門氏菌是一種常見的食源性人畜共患腸道致病菌，兼性胞內致病菌。山東省某養驢場頻發流產現象且無明顯征兆，本研究對驢流產胎兒內臟及頭部感染細菌進行分離，并對分離株進行鑒定及耐藥、毒力分析。結果表明，獲得的7株分離株均為沙門氏菌，分別命名為SL1-SL7，其中SL1、SL2、SL3、SL5、SL6與鼠傷寒沙門氏菌的同源性分別為100%、99.93%、99.93%、99.79%、99.57%;SL4與愛丁堡沙門氏菌、湯姆遜沙門氏菌的同源性均為99.79%;SL7與阿邦尼沙門氏菌、愛丁堡沙門氏菌、湯姆遜沙門氏菌同源性分別為99.72%、99.72%、99.65%;耐藥表型結果顯示7株沙門氏菌對本試驗中所有藥物均比較敏感;毒力基因篩選結果顯示，除SL1、SL2、SL3、SL7 毒力島SPI-4上的ssb毒力基因未檢出外，7株分離菌均具有沙門氏菌典型毒力基因;7株沙門氏菌對小鼠的致病性較弱，小鼠喂食菌液后未出現死亡現象。

關鍵詞：沙門氏菌;驢;分離鑒定;耐藥分析;毒力基因

中圖分類號：S858.22 文獻標識號：A 文章編號：1001-4942（2019）11-0143-06

Isolation， Identification， Drug Resistance and

Virulence Analysis of Salmonella from Donkey

Sun Yangyang， Yang Mei， Xie Xiaoli， Cheng Kaihui，

Zhang Liang， Chu Huimeng， Ren Yachu， Yang Hongjun

（Dairy Cattle Research Center， Shandong Academy of Agricultural Sciences， Jinan 250131， China）

Abstract Salmonella is a common foodborne zoonotic enteric pathogen and facultative intracellular pathogen. There was no obvious sign of frequent abortion in a donkey factory in Shandong Province. In this study， the infection bacteria in viscera and head were isolated， and identifying the isolates and analyzing their drug resistance and virulence. The results showed that all the 7 isolates identified were Salmonella and named as SL1-SL7. The homology rates of SL1， SL2， SL3， SL5 and SL6 with Salmonella typhimurium were 100%， 99.93%， 99.93%， 99.79% and 99.57%， respectively. The homology rates of SL4 with Salmonella edinburgh and Salmonella thomson were 99.79%. The homology rates of SL7 with Salmonella arbonini， Salmonella edinburgh and Salmonella thomson were 99.72%， 99.72% and 99.65%， respectively. The results of drug-resistant phenotype showed that 7 strains of Salmonella were sensitive to all the drugs in this study. The results of virulence gene screening showed that all the 7 isolates had typical virulence genes of Salmonella except the ssb virulence genes on SPI-4 of SL1， SL2， SL3 and SL7 virulence islands. The 7 strains of Salmonella were less pathogenic to mice， and the mice did not die after feeding the bacteria solution.

Keywords Salmonella; Donkey; Separation and identification; Resistance analysis; Virulence gene

沙門氏菌（Salmonella enteriditis，SE）屬腸桿菌科 （Enterobacteriaceae），革蘭氏陰性腸道桿菌，兼性胞內致病菌，是一種常見的食源性人畜共患腸道致病菌，其中畜禽感染非常普遍，主要引起發熱、腹瀉、敗血癥、胃腸炎和腦膜炎等癥狀[1， 2]，造成懷孕母畜發生流產，嚴重時還可導致死亡，對人和動物的健康威脅巨大。因此，沙門氏菌的檢測和預防治療在醫學、獸醫學和公共衛生方面都有十分重要的意義。豬、雞等動物沙門氏菌病較為常見，而關于馬屬動物特別是驢的沙門菌病國內鮮少報道。……