五磨飲對脾虛氣滯證功能性消化不良大鼠的干預作用

甘國興 吳曉芳 馮偉勛 鄺衛紅 李勁平 謝保平 張毅之

[摘要] 目的 研究五磨飲對脾虛氣滯證功能性消化不良（FD）大鼠的干預作用。 方法 70只雌性SD大鼠隨機選出10只作為正常組，其余采用“慢性束縛+游泳+飲食不節”復合因素法制備脾虛氣滯證FD模型。48只造模成功大鼠隨機分為模型組、多潘立酮組、柴芍六君子湯組、五磨飲高劑量組、五磨飲中劑量組、五磨飲低劑量組，每組8只。除正常組及模型組給予等體積純凈水外，其余大鼠灌胃給藥干預14 d。記錄大鼠食量和體質量，測定小腸推進率，檢測胃竇及下丘腦組織誘導型一氧化氮合酶（iNOS）、P物質（SP）、5-羥色胺（5-HT）表達。 結果 與模型組比較，給藥第5、10、15天，正常組、各給藥組大鼠食量顯著增加（P < 0.05或P < 0.01）。與模型組比較，給藥第5天，正常組、多潘立酮組大鼠體質量顯著增加（P < 0.05或P < 0.01）;與模型組比較，給藥第10、15天，正常組、各給藥組大鼠體質量顯著增加（P < 0.05或P < 0.01）。與模型組比較，正常組、各給藥組大鼠小腸推進率顯著增加（P < 0.01）。與模型組比較，各給藥組、正常組大鼠胃竇組織iNOS表達顯著下調（P < 0.05）;與模型組比較，多潘立酮組、五磨飲低劑量組大鼠胃竇組織SP表達顯著下調（P < 0.01）;與模型組比較，五磨飲高劑量組大鼠胃竇組織5-HT表達上調（P < 0.05）。與模型組比較，正常組、多潘立酮組、柴芍六君子湯組、五磨飲中劑量組大鼠下丘腦iNOS表達顯著升高（P < 0.05或P < 0.01）;與模型組比較，正常組、柴芍六君子湯組、五磨飲中劑量組大鼠下丘腦SP表達顯著升高（P < 0.05或P < 0.01）。 結論 五磨飲對脾虛氣滯證FD模型大鼠具有一定的療效，調節腦-腸軸iNOS、SP、5-HT表達可能是其作用機制之一。

[關鍵詞] 功能性消化不良;五磨飲;藥效;腦腸肽

[中圖分類號] R318.14? ? ? ? ? [文獻標識碼] A? ? ? ? ? [文章編號] 1673-7210（2019）07（a）-0020-05

Intervention effect of Wumoyin Decoction on functional dyspepsia rats of spleen deficiency and Qi stagnation syndrome

GAN Guoxing1? ?WU Xiaofang2? ?FENG Weixun1? ?KUANG Weihong3? ?LI Jingpin4? ?XIE Baopin5? ?ZHANG Yizhi1

1.Department of Pharmacy， Qingyuan Hospital of TCM， Guangdong Province， Qingyuan? ?511500， China; 2.School of Nursing， Qingyuan Polytechnic， Guangdong Province， Qingyuan? ?511510， China; 3.the First Affiliated Hospital of Guangzhou University of TCM， Guangdong Province， Guangzhou? ?510006， China; 4.College of Pharmacy， Central South University， Hu′nan Province， Changsha? ?410013， China; 5.Key Laboratory， Prevention and Treatment of Cardiovascular and Cerebrovascular Disease of Ministry of Education of Gannan Medical University， Jiangxi Province， Ganzhou? ?341000， China

[Abstract] Objective To study the effect of Wumoyin Decoction on FD rats of spleen deficiency and Qi stagnation syndrome. Methods Ten rats were randomly selected as normal group from 70 female SD rats， and other rats were modeled by "chronic restraint + swimming + irregular eating" compound factor method. 48 modeled rats were randomly divided into model group， Domperidone group， Chaishao Liujunzi Decoction group， Wumoyin Decoction high， middle and low dose group， 8 rats in each group. Besides normal group and model group， the other groups of rats were given the corresponding drug intervention for 14 days. The food intake and body weight were recorded， the small intestine propulsion rate was determined， the expression of iNOS， SP， 5-HT in antral tissue and hypothalamus were detected. Results Compared with model group， the food intake of rats in normal group and administration groups were increased significantly on the 5th， 10th and 15th day of administration （P < 0.05 or P < 0.01）. Compared with model group， body mass of rats in normal group and Domperidone group were increased significantly on the 5th day of administration （P < 0.05 or P < 0.01）. Compared with model group， body mass of rats in normal group and administration groups were increased significantly on the 10th and 15th day of administration （P < 0.05 or P < 0.01）. Compared with model group， the propulsive rate of small intestine in normal group and administration groups were increased significantly （P < 0.01）. The expression of iNOS in gastric antrum tissue of rats in administration groups and normal groups were significantly down-regulated compared with model group （P < 0.05）. Compared with model group， SP expression in gastric antrum tissue of Domperidone group and Wumoyin low dose group rats were significantly deed （P < 0.01）. Compared with the model group， the expression of 5-HT in gastric antrum tissue of rats in high dose group of Wumoyin was up-regulated （P < 0.05）. Compared with model group， the expression of iNOS in hypothalamus of normal group， Domperidone group， Chaishao Liujunzi Decoction group and Wumoyin medium dose group were significantly increased （P < 0.05 or P < 0.01）. Compared with model group， the expression of SP in hypothalamus of normal group， Chaishao Liujunzi Decoction group and Wumoyin medium dose group rats was significantly increased （P < 0.05 or P < 0.01）. Conclusion Wumoyin Decoction has certain curative effect on FD rats of spleen deficiency and Qi stagnation syndrome， regulating the expression of iNOS， SP and 5-HT in the brain-gut axis may be one of its mechanisms.