濟生腎氣丸加味對順鉑所致大鼠急性腎損傷的影響

丁斗 董小君 敖春

【摘 要】 目的：觀察濟生腎氣丸加味對順鉑所致大鼠急性腎損傷的影響，并探討其可能的作用機制。方法：將60只Wistar大鼠隨機分為6組：正常對照組、模型對照組、陽性對照組、濟生腎氣丸加味低、中、高劑量治療組。除正常對照組外，其他組大鼠均1次性腹腔注射7mg·kg-1順鉑造模。濟生腎氣丸加味各劑量治療組分別給予濟生腎氣丸加味水煎液10、20、40g·kg-1灌胃，連續(xù)14d。實驗第15天，采集血液標本，檢測大鼠血清BUN、Cr、SOD、MDA含量；留取腎組織，測定腎臟系數(shù)，并采用ELISA法檢測大鼠腎組織中TNF-α、TGF-β1含量，HE染色觀察腎臟組織病理學變化。結果：與正常對照組比較，模型對照組血清BUN、Cr、MDA 含量明顯上升，SOD活力顯著下降，腎組織中TNF-α、TGF-β1含量與腎臟系數(shù)升高，腎臟病理損傷嚴重，腎小球囊腔增大，腎小管上皮細胞變性、壞死脫落，管腔內出現(xiàn)蛋白管型。濟生腎氣丸加味各劑量治療組上述指標較模型對照組明顯改善。結論：濟生腎氣丸加味對順鉑所致的大鼠腎損傷有保護作用，其作用機制可能與其具有抗氧化作用，下調炎癥細胞因子表達，延緩成纖維細胞發(fā)生纖維化等有關。

【關鍵詞】 濟生腎氣丸加味；順鉑；腎損傷；大鼠

【中圖分類號】R285.5 【文獻標志碼】 A 【文章編號】1007-8517（2018）01-0048-05

Abstract：Objective To observe the effects of Jisheng Shenqi Wan Jiawei on acute renal injury induced by cisplatin in rats and to explore its possible mechanism. Methods 60 Wistar rats were randomly divided into 6 groups：the normal control group，the model control group，the positive control group，the low，medium，high dose Jisheng Shenqi Wan Jiawei treatment group. All the other groups were injected with 7 mg·kg-1 cisplatin by intraperitoneal injection except the normal control group. Each dose treatment group was given 10，20，40 g·kg-1 Jisheng Shenqi Wan Jiawei decoction by intragastric administration for 14 consecutive days. The blood and kidney samples were collected on the fifteenth day. Serum BUN，Cr，SOD，MDA and renal index were detected. The content of TNF-α and TGF-β1 in renal tissues were detected by enzyme linked immunosorbent assay（ELISA）. Histopathologic？changes of kidney were observed by hematoxylin-eosin（HE） staining. Results Compared with the normal control group，serum BUN，Cr，MDA of the model control group were markedly increased. The activity of serum SOD was decreased significantly. The content of TNF-α，TGF-β1 and renal index were obviously increased. Renal pathological damage was severe. The glomerular cysts were enlarged. Renal tubular epithelial cells appeared degeneration，necrosis and shedding. The tube cavity appeared protein tube type. The above indicators were improved significantly in each dose Jisheng Shenqi Wan Jiawei treatment group than those in the model control group. Conclusions Jisheng Shenqi Wan Jiawei has protective effect on renal injury induced by cisplatin in rats. The mechanism may be related to its antioxidation，down regulation of inflammatory cytokine expression and delaying fibrogenesis.

Keywords：Jisheng Shenqi Wan Jiawei；Cisplatin；Renal Injury；Rat

順鉑（Cisplatin，DDP）是目前臨床上廣泛應用于多種實體瘤的一線鉑類抗腫瘤藥物[1]。但隨著順鉑劑量的增加，毒副作用亦增高，特別是腎毒性成為順鉑的劑量限制性[2]。因此，保留順鉑的抗癌作用，降低其腎毒性，是保證化療效果的重要環(huán)節(jié)，也是多年來藥理學、毒理學和臨床治療學等關注的熱點。……