3種三萜類化合物對(duì)黃胸散白蟻生物活性及纖維素酶的影響

徐偉麗+韓萌+袁忠林

摘要：為明確馬纓丹葉片中分離提純的馬纓丹酸、馬纓丹烯A和馬纓丹烯B 3種三萜類化合物對(duì)白蟻的毒殺活性及對(duì)其體內(nèi)纖維素酶的影響，采用濾紙片飼喂法測(cè)定了3種化合物對(duì)黃胸散白蟻的毒殺作用，并用酶標(biāo)儀測(cè)定了不同濃度、不同處理時(shí)間白蟻體內(nèi)內(nèi)切葡聚糖酶（EG）、外切葡聚糖酶（CBH）、β-葡萄糖苷酶（BGL）活性。結(jié)果表明，3種三萜類化合物對(duì)白蟻具有一定的毒殺作用。酶活性測(cè)定表明，不同濃度馬纓丹酸處理黃胸散白蟻12～72 h，白蟻體內(nèi)的EG、CBH、BGL活性均有不同程度的變化，其對(duì)白蟻體內(nèi)EG活性總體有抑制作用，2 mg/mL在12～36 h抑制作用最為顯著；對(duì)CBH活性則有一定的促進(jìn)作用，2 mg/mL 對(duì)CBH活性促進(jìn)作用較為明顯，但與對(duì)照相比差異不顯著；對(duì)BGL活性則表現(xiàn)為抑制作用。馬纓丹烯B和馬纓丹烯A對(duì)黃胸散白蟻體內(nèi)3種酶活性均表現(xiàn)為抑制作用，且隨濃度的增大抑制作用顯著。因此，3種三萜類化合物對(duì)黃胸散白蟻體內(nèi)的纖維素酶活性均有一定影響，尤其是馬纓丹烯B、A抑制作用較強(qiáng)，它們對(duì)3種酶活性的影響與濃度和時(shí)間有關(guān)。

關(guān)鍵詞：馬纓丹；馬纓丹酸；馬纓丹烯A；馬纓丹烯B；黃胸散白蟻；纖維素酶；生物活性

中圖分類號(hào)：S433文獻(xiàn)標(biāo)識(shí)號(hào)：A文章編號(hào)：1001-4942（2017）11-0098-07

Effects of 3 Triterpenoids on Bioactivity and Cellulase

Activity of Reticulitermes flaviceps （Isoptera： Rhinotermitidae）

Xu Weili， Han Meng， Yuan Zhonglin

（College of Plant Health and Medicine， Qingdao Agricultural University/Key Laboratory of

Integrated Crop Pest Management of Shandong Province， Qingdao 266109， China）

AbstractIn order to define the bioactivity and effects on the cellulose activity of termite， three triterpenoids， lantanolic acid， lantadene A and B， which isolated and purified from leaves of Lantana camara， were used to feed Reticulitermes flaviceps by filter paper method. The activities of 3 enzymes of endo-1，4-β-glucanase （EG）， exo-β-1，4-glucanase（CBH） and β-1，4-glucosidase （BGL） in termite were tested with ELIASA at different concentrations and treatment times. The results showed that the 3 triterpenoids all had certain toxic activity on termite. The enzyme activity test showed that EG， CBH and BGL all had different changes when treated with lantanolic acid for 12～72 hours. In general， lantanolic acid had an inhibitory effect on EG， and it was significant at the concentration of 2 mg/mL in 12～36 hours. Lantanolic acid had certain active effect on CBH， and the effect of 2 mg/mL was more obvious， but it was not significantly different compared with control. On BGL， lantanolic acid showed an inhibitory effect. Lantadene A and B showed inhibitory effects on all three kinds of enzymes， and the inhibitory effects became significant with the increase of concentration. Therefore， three triterpenoids all had certain effects on the cellulase activity in Reticulitermes flaviceps which depended on the concentration and treatment time， especially the inhibitory effect of lantadene B and A.

KeywordsLantana camara； Lantanolic acid； Lantadene A； Lantadene B； Reticulitermes flaviceps； Cellulase； Bioactivity

白蟻（termite）是世界性五大害蟲（須舌蠅、蜱、蚊子、粘蟲和白蟻）之一，總數(shù)不少于2 500種，遍布全世界，特別是熱帶、亞熱帶國(guó)家，為害面積約占全球總面積的50%[1]。白蟻為害十分廣泛，如房屋建筑、園林綠化苗木、鐵道枕木、船舶橋梁、江河堤圍、水庫(kù)土壩、地下電纜、經(jīng)濟(jì)林木、名勝古跡、倉(cāng)庫(kù)物資、文物檔案、家具衣服、室內(nèi)裝飾物等。……