野茼蒿對UC大鼠血清黏附分子表達的影響

彭凡珂　馮陳慧　寇力丹

【摘要】 目的：通過研究野茼蒿對潰瘍性結腸炎大鼠血清相關黏附分子表達的影響，探討其對潰瘍性結腸炎的療效。方法：選擇2，4，6-三硝基苯磺酸（TNBS）與乙醇混合液誘導的潰瘍性結腸炎大鼠為研究對象，把UC模型大鼠分為：空白對照組、模型組，野茼蒿低、中、高劑量組，陽性對照組，每組各12只。每天給予各組不同藥物處理。于給藥21 d后對大鼠結腸組織進行CMDI和HS評分，并用ELISA法檢測血清黏附分子CD62P、PSGL-1、CD44、CD54的含量，比較各組間指標差異。結果：與空白組相比，模型組CMDI評分和HS評分以及血清中黏附分子CD62P、PSGL-1、CD44、CD54明顯升高（P<0.01或P<0.05）；與模型組比較，低、中、高劑量組大鼠上述兩個評分以及黏附分子含量一定程度上降低（P<0.01或P<0.05）；與模型組相比，SASP組大鼠血清述兩個評分以及黏附分子的含量明顯降低（P<0.01或P<0.05）。結論：野茼蒿凍干粉對UC大鼠有一定的治療作用，其機制可能與影響CD62P、PSGL-1、CD44、CD54的表達有關。

【關鍵詞】 潰瘍性結腸炎； 野茼蒿； 血清黏附分子

【Abstract】 Objective：To investigate the effect of Wild Chrysanthemum on the expression of serum-related adhesion molecules in rats with ulcerative colitis and to explore its effect on ulcerative colitis.Method：The rats of ulcerative colitis induced by 2，4，6-trinitrobenzene sulfonic acid（TNBS） and ethanol were selected as the study subjects.The UC rats were divided into six groups：blank group，model group，wild chrysanthemum Low，medium and high dose group，positive control group，12 cases in each group.Each group was given a different medication every day.At 21 days after administration，CMDI score and HS score were performed on rat colon tissue.The levels of CD62P，PSGL-1，CD44 and CD54 in serum were measured by ELISA.The differences of six groups were compared and analyzed statistically.Result：Compared with the blank group，the CMDI scores and HS scores and the levels of CD62P，PSGL-1，CD44 and CD54 in the serum of the model group were significantly increased（P<0.01 or P<0.05）；compared with the model group，the two scores and the levels of adhesion molecules in serum of the low，medium and high dose rats groups were significantly lower（P<0.01 or P<0.05）；compared with the model group，the above two scores and the content of adhesion molecules in serum of SASP group were significantly decreased（P<0.01 or P<0.05）.Conclusion：The freeze-dried powder of Wild Chrysanthemum has a certain therapeutic effect on the clinical symptoms of UC rats.The mechanism may be related to the expression of CD62P，PSGL-1，CD44 and CD54.

【Key words】 Ulcerative colitis； Wild Chrysanthemum； Serum adhesio lecules

First-authors address：Qiqihar Medical University，Qiqihar 161006，China

doi：10.3969/j.issn.1674-4985.2017.25.005

潰瘍性結腸炎（Ulcerative Colitis，UC）是一種結腸和直腸慢性非特異性炎癥性疾病，病因尚不明確，病變范圍廣泛。目前大多認為其發病機制與免疫因素、遺傳因素、心理因素、飲食因素等有關，其中免疫異常被認為是最重要的發病因素。潰瘍性結腸炎治愈難度大、病程長，經常反復發作，UC的發病率在我國呈升高的趨勢。目前UC臨床治療以激素、水楊酸制劑和免疫抑制劑為主，但療效欠佳，患者易產生耐藥性、過敏等不良反應。中藥在治療UC上具有一定優勢，避免了傳統西藥的弊端，在UC的治療上顯示出了廣闊前景，臨床上以中藥灌腸治療效果較好[1-2]。野茼蒿（Wild chrysanthemum）是菊科茼蒿屬一年或二年生草本植物，在我國境內主要分布于廣東、海南、四川、云南等地，是一種常用的藥食兩用植物，含有多種類型化合物，可治療慢性腸胃炎[3-5]。黏附分子CD62P、CD44、CD54參與炎癥反應中的重要環節，在UC的發病過程中起到了重要作用。……