桑杏清營湯對放射性肺炎肺組織TGF—β1表達的影響研究

劉毅+由鳳鳴+嚴然+張曉丹

摘要：目的觀察桑杏清營湯對放射性肺炎大鼠肺組織中TGF-β1表達的影響，探討成都中醫藥大學附屬醫院防治放射性肺炎的經驗方桑杏清營湯對大鼠放射性肺炎（Radiation Pneumonitis，RP）的療效及作用機制。方法將180只8周齡、體重約為280 g～300 g的SPF級雄性SD大鼠，隨機分為空白對照組（空白組），照射模型組（模型組），西藥治療組（陽性組），中藥預防組（預防組），中藥治療組（治療組），每組36只。除外空白組均給予直線加速器6MV-X線，20 gy照射1次。除預防組于照射前4天開始灌服桑杏清營湯外，其余各組造模后第二天開始，空白組、模型組給予等容積的生理鹽水灌胃，治療組灌服桑杏清營湯，陽性組給予醋酸潑尼松混懸液，每日1次，5d/周。照射后第2、4、8、12周每組隨機取9只。將右肺組織固定于福爾馬林溶液中，石蠟包埋，切片，HE染色光鏡下觀察肺組織病理形態學變化，免疫組化法檢測TGFβ1表達。結果光鏡下模型對照組較正常組肺組織炎癥細胞浸潤、結構破壞明顯，模型組與空白組大鼠比較肺組織TGF-β1的表達顯著升高（P<0.01），提示造模成功；與桑杏清營湯相比，模型組肺組織TGF-β1的表達升高（P<0.05），其中桑杏清營湯預防組（P<0.01）；結論桑杏清營湯防治放射性肺炎可能是通過抑制TGF-β1/Smad信號轉導通路，從而抑制大鼠肺組織中TGF-β1的表達，延緩并減輕肺纖維化。

關鍵詞：放射性肺炎；桑杏清營湯；肺組織；TGFβ1；TGF-β1/Smad通路

中圖分類號：R285.5文獻標志碼：A文章編號：1007-2349（2017）04-0078-04

Study on the Effect of Sangxingqingying Decoction on Expression

of TGF-β1 in Radiation Pneumonia Tissue

LIU Yi1，3， YOU Feng-ming1， YAN Ran1， ZHANG Xiao-dan2

（1. Chengdu University of Traditional Chinese Medicine， Chengdu 610075， Sichuan；

2. Department of Basic Medical Sciences， Chengdu University of Traditional Chinese Medicine， Chengdu 610075， Sichuan；

3. Sichuan Hospital of Traditional Chinese Medicine and Western Medicine， Chengdu 610041， Sichuan）

【Abstract】Objective： To observe the effect of Sangxingqingying Decoction on the expression of TGF-β1 in radiation pneumonia tissue of rats and to explore its curative effect and mechanism of treating radiation pneumonitis （RP） in the Affiliated Hospital to Chengdu University of Traditional Chinese Medicine. Methods： 180 male SD rats at the age of 8-week old and weighing about 280 g to 300g were randomly divided into a blank control group （blank group）， irradiated model group （model group）， western medicine treatment group （positive group ）， Chinese medicine prevention group （prevention group） and traditional Chinese medicine treatment group （treatment group）， 36 rats each group. Except the blank group， all other groups were given linear accelerator 6MV-X line， 20 gy irradiation 1 time. Except the prevention group that was administrated with Sangxingqingying Decoction 4 days before radiation， the rest groups were given Sangxingqingying Decoction in the next day after modeling. The blank group and the model group were given the same volume of saline. The treatment group was given with Sangxingqingying Decoction and the positive group was given prednisone acetate suspension， once a day， 5 days a week. At the 2nd， 4th， 8th and 12th week after irradiation， 9 rats were randomly taken from each group. The right lung tissue was fixed in formalin solution， paraffin embedded and sliced. HE staining was used to observe the pathological changes of lung tissue， and immunohistochemical method was used to detect TGFβ1 expression. Results： Compared with the control group， the structural damage of the model group was obvious in the lung tissue inflammation cells infiltration under light microscope. Compared with the control group， the expression of TGF-β1 in lung tissue of the model group and the blank group significantly increased （P<0.01）， suggesting that the model was successful. Compared with SangXiangxingqingying Decoction group， the expression of TGF-β1 in lung tissue of the model group increased（P<0.05）， including the prevention group（P<0.01）. Conclusion： The prevention and treatment of radiation pneumonia with Sangxingqingying Decoction may inhibit the expression of TGF-β1 in the rat lung tissue through the inhibition of TGF-β1/Smad signal transduction pathway， and delay and reduce pulmonary fibrosis.