高粘度快速發酵生產燃料乙醇技術研究最終報告

靳艷玲 趙海 方揚

摘 要：在粘度產生機制方面，以甘薯為模式原料利用多糖單克隆抗體芯片分析技術解析了粘度產生的機制，為降粘技術的開發提供了靶點；在降粘技術方面，利用商品化酶開發了復合降粘酶系，同時還自主篩選出了可以同時產生復合降粘酶的單菌株，并開發了配套的降粘工藝，可使甘薯、木薯、芭蕉芋等粘度下降90%以上；在菌種選育方面，選育到8株高濃度乙醇發酵酵母，并通過適應性馴化提高了菌株對高溫、高壓等環境壓力條件的抗性，同時還研究了菌株的壓力耐受機制，為進一步改造或優化其應用工藝提供數據支持；在發酵過程調控方面，開發出了代謝促進劑及配套的高濃度快速乙醇發酵技術，發酵24 h內，乙醇濃度可達12%以上；在反應器開發方面，開發出新型利用變頻泵驅動的高傳質低能耗反應器，可使粘度高達9萬mPa·S的薯類濃醪傳質均勻；通過高效乙醇發酵菌株，降粘技術體系，快速乙醇發酵和高濃度乙醇發酵等關鍵技術模塊的系統集成建立了高粘度快速發酵生產燃料乙醇技術體系，并應用于西南地區最大的乙醇生產企業——資中縣銀山鴻展工業有限責任公司3萬t燃料乙醇示范生產線上。以鮮甘薯為原料，發酵時間由現有技術的60 h以上縮短為30 h以內，乙醇濃度由5%～6%（v/v）平均提高到10.69%（v/v），最高可達12.41%（v/v），發酵效率由88%以下提高至90%以上，可提高單位設備的生產力，降低乙醇蒸餾能耗，節能效果明顯。

關鍵詞：燃料乙醇 粘度 非糧

Abstract：In terms of the mechanism of viscosity， sweet potato was used as a model material and investigated with polysaccharide monoclonal antibody microarray. The results of this analysis explained what kinds of component related with viscosity and provided targets for viscosity reduction technology. Complex enzymes was developed for viscosity reduction， and fungi which could produce complex viscosity reduction enzymes was screened. Under the function of these enzymes， viscosity of sweet potato，cassava and canna was reduced by 90%. In terms of microbe， 8 strains of yeast were screened， and then acclimation to high temperature and high concentration of ethanol was carried out. The mechanism of stress tolerance of these yeasts was investigated， and the results would be benefit to better performance of these yeasts. In terms of fermentation technology， one fermentation stimulant was developed. Under the function of this stimulant，12% ethanol could be produced within 24h. In terms of reactor， novel reactor with high performance and low energy consumption was developed， which could blend high viscosity sweet potato mash well. After system integration， these technologies were applied to 30，000t scale of production line in the biggest ethanol plants in southwest of China. Fermentation time was reduced from more than 60h to less than 30h， ethanol concentration was increased from 5%～6% to 12%， and ferment efficiency was enhanced from 88% to 90%. These changes of parameters could enhance productivity of facilities， and decrease energy consumption significantly.

Key Words：Fuel Ethanol；Viscosity；Non-grain Material

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