玉米骨干自交系深度重測(cè)序及有利基因挖掘

金危危 賴錦盛 張祖新 高世斌

摘 要：通過(guò)我國(guó)玉米骨干自交系的全基因組序列圖譜、轉(zhuǎn)錄組圖譜、基于基因的SNP單倍體型圖譜等玉米基因組的研究以及玉米重要農(nóng)藝和經(jīng)濟(jì)性狀的全基因組關(guān)聯(lián)分析，批量鑒定和發(fā)掘玉米重要性狀的功能基因，推動(dòng)我國(guó)玉米的基礎(chǔ)研究和分子育種研究，最終促進(jìn)我國(guó)玉米產(chǎn)業(yè)的發(fā)展。對(duì)10份我國(guó)典型優(yōu)良玉米自交系全基因組進(jìn)行重測(cè)序分析以及100份典型溫帶玉米自交系籽粒發(fā)育轉(zhuǎn)錄組序列分析，分析我國(guó)典型溫帶玉米自交系基因組的多樣性和遺傳結(jié)構(gòu)；挖掘玉米自交系籽粒發(fā)育轉(zhuǎn)錄組的特征；構(gòu)建玉米高密度的基于基因的SNP單倍體型圖譜和表達(dá)譜（eQTL）；結(jié)合關(guān)聯(lián)分析等方法批量鑒定和發(fā)掘影響玉米重要性狀如品質(zhì)、抗病性等的重要基因，明確這些基因的功能、作用機(jī)理、各基因的優(yōu)良等位基因及其玉米種質(zhì)材料，開發(fā)一批可用于玉米分子育種、效應(yīng)明顯的功能標(biāo)記。

關(guān)鍵詞：玉米 自交系 重測(cè)序 關(guān)聯(lián)分析

Abstract：Through maize genome research （such as our country maize elite inbred lines whole genome sequencing， the transcriptome map， the SNP haplotype map） as well as Genome-wide association study of maize important agronomic and economic characters， this projects aims at identifying and exploring the function gene of maize important characteristics， promoting maize basic research and molecular breeding research， so as to accelerate the development of maize industry in China. Through 10 maize elite inbred lines whole genome resequencing analysis and 100 typical temperate maize inbred lines kernel development transcriptome sequences analysis， we analyzing the genome diversity and genetic structure of the typical temperate maize inbred lines in our country； exploring the feature of kernel development transcriptome of maize inbred lines； constructing maize high-density SNP haplotype map and expression spectrum （eQTL）； identifying the function gene of affecting maize important traits such as quality， disease resistance combining with correlation analysis methods； grasping the function of these genes， mechanism， good alleles and maize germplasm materials； and developing a batch of effect obvious function markers using for maize molecular breeding.

Key Words：Maize；Inbred line；Resequencing；Association Analysis

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