微生物過氧化氫酶的發酵生產及其在紡織工業的應用

張東旭，堵國成,，陳堅

1 江南大學 工業生物技術教育部重點實驗室，無錫 214122

2 江南大學 食品科學與技術國家重點實驗室，無錫 214122

綜 述

微生物過氧化氫酶的發酵生產及其在紡織工業的應用

張東旭1，堵國成1,2，陳堅2

1 江南大學 工業生物技術教育部重點實驗室，無錫 214122

2 江南大學 食品科學與技術國家重點實驗室，無錫 214122

微生物過氧化氫酶是一種重要的工業酶制劑，可以催化分解過氧化氫生成水和氧氣。這一酶制劑在食品、紡織、醫藥等領域表現出廣泛的應用潛力。生物工程和基因工程技術的進步推動了微生物過氧化氫酶的發酵生產。以下綜述了微生物過氧化氫酶發酵生產的進展及其在紡織工業中的應用，同時討論了微生物過氧化氫酶的發酵生產和紡織工業應用的未來趨勢。

微生物過氧化氫酶，發酵，紡織，應用

Abstract:Microbial catalase is an important industrial enzyme that catalyzes the decomposition of hydrogen peroxide to water and oxygen. This enzyme has great potential of application in food, textile and pharmaceutical industries. The production of microbial catalase has been significantly improved thanks to advances in bioprocess engineering and genetic engineering. In this paper, we review the progresses in fermentation production of microbial catalase and its application in textile industry. Among these progresses, we will highlight strain isolation, substrate and environment optimization, enzyme induction, construction of engineering strains and application process optimization. Meanwhile, we also address future research trends for microbial catalase production and its application in textile industry. Molecular modification (site-directed mutagenesis and directed revolution) will endue catalase with high pH and temperature stabilities. Improvement of catalase production, based on the understanding of induction mechanism and the process control of recombinant stain fermentation, will further accelerate the application of catalase in textile industry.

Keywords:microbial catalase, fermentation, textile, application

1 過氧化氫酶簡介

過氧化氫酶 (Hydrogen peroxide oxidoreductase，catalase EC 1.11.1.6.) 是一類以過氧化氫為專一底物，通過催化一對電子的轉移而最終將其降解為水和氧氣的酶。研究表明幾乎所有的需氧微生物中都存在過氧化氫酶，只有少數好氧菌如過氧化醋桿菌Acetobacter peroxydas不存在過氧化氫酶[1-4]。除謝氏丙酸桿菌Propionibacterium shermanji和巨大脫硫弧菌Desulfovibrio gigas等微生物外，絕大多數厭氧微生物體內不存在過氧化氫酶[5]。根據過氧化氫酶在結構和序列水平上的異同將其劃分為3個亞群，即單功能過氧化氫酶 (Monofunctional catalase or Typical catalase)、雙功能過氧化氫酶 (Catalase-peroxidase) 和假過氧化氫酶 (Pseudocatalase or Mn-catalasee)。大多數的過氧化氫酶由 4個相同的亞單位組成，分子量在240 kDa左右，在亞基的活性部位各含一個血紅素基團[6]。……