Visual acuity of urban and rural adults in a coastal province of southern China: the Fujian Eye Study

INTRODUCTION

With improvements in living standards and quality of life, improvement in visual function has become a hot topic. Visual acuity (VA) examination is an important content of a complete oculo-visual evaluation and is very important in the test of amblyopia, refractive error and ocular disease. One of the key objectives of Universal Eye Health:A Global Action Plan 2014-2019 is to generate evidence of the magnitude of vision impairment (VI), which affects economic and educational opportunities, reduces quality of life, and increases the risk of death of approximately 441.1 million persons. Right to Sight is also a very important global project in ophthalmology, launched by WHO in 1999. The project target is set to decrease the expected doubling of blind patients worldwide by the year 2020 as a result of decrease in both mortality and fertility prevalence with the population aging rapidly in most countries. Recently, many reports displayed strong association between socioeconomics and prevalence and causes of VI and blindness which attract great interest to ophthalmologist and policy makers of public eye health. Our study just conducted from 2018-2019, and could provide the latest prevalence and related factors of VI and eye diseases.

Many articles have reported the prevalence and risk factors for VA changes or VI worldwide, but few published studies have compared and analyzed VA and VI in both rural and urban regions and inland and coastal areas. Due to the specific geographic location, six cities in Fujian Province have coastlines and three cities are inland areas, differences between both rural and urban regions and inland and coastal areas can be explored at the same time. Therefore, the present Fujian Eye Study (FJES) aimed to evaluate the central VA as an important factor to visual function among rural and urban and inland and coastal populations in southern China and identify ocular and demographic associations. As we know, there are many factors that affect vision, not only the eyes, but also the physical body, even socio-economic and demographic factors.Many studies have reported the related factors a long time ago,such as age, sex, income, education, and refractive error, while these studies were not up to date and limited. In order to fill this gap in knowledge and to comprehend the vision health status and factors correlated with major eye diseases and vision loss, the research group conducted a population-based cross-sectional investigation to obtain data about the eye health situation among Chinese individuals aged 50y and above living in Fujian Province. This survey was just operated before the COVID-19 outbreak, and this may guide the eye health policymaking in future.

SUBJECTS AND METHODS

Based on the WHO definitions considering BCVA, 196 (2.88%)residents were low vision, and 31 (0.39%) residents were blind.The response rate of the whole population was 81.8% (8211 out of 10 044), and 4836 were female. Totally 43.0% of the population was from a rural area, which was similar to the rural and urban population ratio in Fujian Province (58.1%41.9%), and 78.4% of the population was from a coastal area,which was also similar to the inland and coastal population ratio in Fujian Province (79.8%20.2%). The response rates in the rural and urban populations were 80.2% and 83.9%and 81.9% and 81.1% for the inland and coastal populations,respectively. The rural and urban groups varied significantly in education and income levels, as did the inland and coastal groups(Table 1).

Study Design The FJES was a population-based, crosssectional investigation on the public eye health status of Fujian Province, Southeast China, including both rural and urban regions and inland and coastal areas. It was carried out to identify the prevalence and related factors associated with VI and ocular diseases in residents aged 50y and above and to comprehend the differences among and obstacles to eye health service use in such areas.

In the Shihpai Eye Study, also a population-based eye study,there was a statistically significant increase in the prevalence of low vision (＜0.001) from 0.83% among 65 to 69y age subgroup to 8.33% among 80y or older subgroup, and there was no statistically significant difference with gender in the rate of blindness or low vision. In the Beijing Eye Study, consistent with the FJES study on southern Chinese individuals, there was also no statistically significant difference with gender in VI prevalence. Interestingly, in a Russian Ural Eye and Medical Study, an increased prevalence of moderate-to-severe VI/blindness was associated with age,male sex and educational level. The Multi-Ethnic Study in White, Chinese, Black and Hispanic Participants showed that older age was statistical significantly correlated with VI in both women and men, especially in those with lower socioeconomic status (SES), while the influences of increasing age on men were more significant than that on women. A national survey among Chinese adults found that older age, young or middleage in males, old age in females, illiteracy, rural dwelling, noneastern residency, single status, unemployment, and lowerincome family status were associated with VI. Our study found that there was no sex difference in BCVA; nevertheless,after age stratification, women over 65y were more likely to have VI than men.

The calculation formula and baseline data used in this study were elaborated in our published article. Assuming a response rate of 80%, to obtain a sufficient sample size according to previous studies, 10 044 subjects were recruited in this study. The total population of Fujian Province (which has an area of 124 000 km) was 38.74 million (permanent population of the province at the end of 2016), including 14.10 million rural population and 24.64 million urban population, or 7.84 million inland population and 30.90 million coastal population.Therefore, 4209 rural residents and 5835 urban residents, or 2190 inland residents and 7854 coastal residents were recruited respectively.

Recruitment Procedures Participants underwent a comprehensive physical examination in a mobile clinic,which was set up in specific location (in community centre,administrative office or hospital). Those who could not participate in on-site examination in the screening were inquired for the consent of home visit and simple ophthalmic examination. All the technicians and clinicians were trained uniformly, and each inspection requires the fixed cooperation of equipment and personnel.

? Sonia Maffei（Cura e Commento），Cesare Ripa’Iconology(1603)，testo stabilito da Paolo Procaccioli Pubblicazione,Torino,Einaudi,2012.

居民地要素更新分為點狀居民地更新和面狀居民地更新。點狀居民地更新可直接選取1∶50 000 DLG數(shù)據(jù)庫中行政村以上居民地進行替換；面狀居民地選取鄉(xiāng)鎮(zhèn)以上行政駐地，因國家下發(fā)的1∶250 000 DLG數(shù)據(jù)的居民地數(shù)據(jù)現(xiàn)勢性較差，參考性不大，故直接采用1∶50 000DLG中的居民地進行綜合。在綜合面狀居民地時，需做到3點：保證圖形不變形，保證不出現(xiàn)小尖角盡量用直角，保證居民地與其他要素的相互關(guān)系。

Correlation of VA with Education In the whole (=0.15 and 0.22,＜0.0001), the same as in the rural residents subgroup(=0.15 and 0.25,＜0.0001), urban residents subgroup(=0.12 and 0.17,＜0.0001), inland residents subgroup(=0.18 and 0.35,＜0.0001) and coastal residents subgroup(=0.14 and 0.21,＜0.0001), both presenting VA and BCVA were significantly associated with education level. Since the urban residents group and inland residents group had a statistical significantly higher educational background than their corresponding groups (Table 1), all the residents were stratified into different subgroups according to the educational background. Figure 1 presents the difference among educational subgroups in detail.

Statistical Analysis Double data entry was performed with EpiData version 3.1 for data collation and the final data was analyzed with Stata/SE statistical software version 15.1.Data are shown as means±standard deviation (SD). Means of normally distributed parameters among different subgroups were compared using analysis of variance (ANOVA).Proportions was compared using Chi-square tests. The relations between VA and selected potential factors was examined using multiple Logistic regression. Normally distributed parameters were compared using linear correlation. Confidence intervals(95%CI) are presented. The statistical strengths of correlations are shown as correlation coefficients () or odd ratio (OR)values. Allvalues＜0.05 were defined statistically significant.

RESULTS

Ethical Approval The Ethics Committee of Xiamen Eye Center affiliated to Xiamen University approved the 2018-2019 FJES protocol (Acceptance number: XMYKZXKY-2018-001) and written informed consent was obtained from all participants.

The main contents of the survey include: general information(name, sex, age, telephone number, ID number, address);questionnaire (race, blood group, the socioeconomic status,disease history, living habits,.); presenting VA; refractive state;best corrected visual acuity (BCVA); slit lamp inspections and fundus inspections [multicolor optical coherence tomography(OCT) and non-mydriatic fundus photographs].

Visual Acuity Only a randomly selected eye per resident among the whole study population was included the statistical analysis. The mean presenting VA measured 0.61 (0.30). When the VA of finger counting (FC) or below were excluded, the mean presenting VA was 0.61 (0.30). When eyes with better VA were selected using in the statistical analysis, the mean presenting VA was 0.68 (0.29). Expressed as the negative logarithmic value of the minimal angle of resolution (logMAR), the mean presenting VA in residents was 0.23 (0.27) logMAR units. The mean BCVA measured 0.82 (0.28). When eyes with a VA of FC or less were excluded, the mean BCVA was 0.83 (0.28).When eyes with better BCVA were selected in the statistical analysis, the mean BCVA values were 0.88 (0.24) and 0.08(0.19) logMAR units.

Regional Comparison Presenting VA was statistical significantly(＜0.0001) greater among the rural residents than among the urban residents [0.62 (0.30)0.58 (0.30)]. Similarly, BCVA was statistical significantly (=0.0001) greater among the urban residents than among the rural residents [0.84 (0.27)0.81 (0.29)]. The presenting VA was statistical significantly(=0.0460) greater among the coastal residents than among the inland residents [0.61 (0.30)0.59 (0.30)]. Similarly, BCVA was statistical significantly (＜0.0001) greater among the coastal residents than among the inland residents [0.84 (0.28)0.78 (0.29); Table 1].

Correlation of VA with Income According to the univariate analysis including the whole study population, presenting VA and BCVA were significantly (=-0.15 and -0.16,＜0.0001)associated with income level. The whole study population was stratified, and the correlations of income level with presenting VA and BCVA were significant in the rural residents group(=-0.17,=0.0013;=-0.22,＜0.0001) and the coastal residents group (=-0.16,=0.0001;=-0.16,＜0.0001). The correlations with BCVA in the urban residents group (=-0.10,＜0.0001) and inland residents group (=-0.22,＜0.0001)were significant, whereas the correlations with presenting VA did not vary significantly in the urban residents group (=-0.09,=0.0867) and inland residents group (=-0.12,=0.1185).Figure 2 shows the difference among income subgroups in detail.

Correlation of VA with Age Presenting VA and BCVA were both significantly associated with age (=-0.33 and -0.42,＜0.0001). Stratifying the present FJES population into rural residents group and urban residents group and inland residents group and coastal residents group showed similar results (urban group:=-0.30 and -0.39,＜0.0001; rural group:=-0.36 and-0.44,＜0.0001; coastal group:=-0.33 and -0.41,＜0.0001;inland group:=-0.31 and -0.46,＜0.0001).

（1）可以采用圓弧邊分析法。所謂3點決定一個圓，1994年7月的325點、2005年6月的998點及2013年6月的1849點可作一圓弧邊，大致上支撐了2018年10月的2449點。但如今又再度接近此圓弧線，恐怕跌破的機會大一些。

Since the rural residents subgroup was statistical significantly younger than the urban residents subgroup (Table 1), both groups were stratified by age. And as the coastal residents subgroup was significantly younger than the inland residents subgroup (Table 1), both subgroups were also randomly stratified by age. Table 2 shows the difference among age subgroups in detail.

Correlation of VA with Refractive Error Presenting VA and BCVA were all significantly associated with refractive error (=-0.20 and -0.27,＜0.001). Stratifying the whole FJES residents into rural residents group and urban residents group and inland residents group and coastal residents group showed similar results (urban group:=-0.22 and -0.20,＜0.0001; rural group:=-0.17 and -0.20,＜0.0001; coastal group:=-0.17 and -0.22,＜0.0001; inland group:=-0.27 and-0.13,＜0.0001). Correspondingly, BCVA was statistical significantly (＜0.0001) lower in the high myopia residents subgroup, excluding the high hyperopia residents subgroup[0.48 (0.33)0.85 (0.25)], with a myopic ametropia exceeding -6.00 diopters, than in non-high myopia group.BCVA also decreased significantly with astigmatism (=-0.27,＜0.0001). And Table 3 shows the difference among refraction groups in detail.

E Standard Logarithmic Visual Acuity Chart (GB 11533—1989) was used to measure presenting VA and BCVA at a distance of 5 meters. We followed the World Health Organization (WHO) definitions of VI with BCVA in better eye worse than 20/60 (equaled to 0.3 in E Chart) and blindness with BCVA in better eye of 20/400 or worse (equaled to 0.05 in E Chart).

(4) P2Y12受體抑制劑的監(jiān)測：有研究表明，根據(jù)血小板功能檢測進行抗血小板治療并不能改善PCI的預(yù)后，不推薦常規(guī)進行血小板功能檢測。

Correlation of VA with Sex and Eye Among the FJES participants, 8063 (98.2%, 8036 out of 8211) had VA test results, including 4776 female residents and 3287 male residents, and 6823 (83.1%, 6823 out of 8211) had BCVA test results, including 4257 female residents and 2566 male residents. In the univariate analyses, presenting VA and BCVA were not significantly different between males and females[0.61 (0.31)0.60 (0.29),=0.1151; 0.82 (0.29)0.83 (0.27),=0.3761]. Moreover, there were no statistically significant difference between right eye and the left eye in presenting VA and BCVA [0.61 (0.30)0.61 (0.30),=0.8625; 0.82 (0.28)0.83 (0.28),=0.4050].

Multiple Regression Analysis Because some of these parameters, such as age and refractive error, there wasstatistical significantly (＜0.0001) association between them,so a multiple linear or logistic regression analysis was needed to be carried out. The common parameters of age, degree of urbanization (urbanrural), geographic location (coastalinland), refraction, education and income were analyzed, and associations with presenting VA and BCVA were significant(＜0.0001) for age, educational background, degree of urbanization and refractive error. Consistent with previous results,sex (=0.194) was not statistical significantly associated with BCVA; conversely, it (＜0.0001) was statistically associated with presenting VA. There was statistically significant difference in income (=0.005) and geographic location(=0.001) with BCVA, whereas they (=0.355 and 0.216)were not significantly correlated with presenting VA.

DISCUSSION

With the increase in the aging population, VI and blindness have become major public health problems worldwide and can impact an individual’s health and quality of life, as well as society. Some previous studies reported the prevalence of VI and its correlations with various factors, for instance, age,ethnic background and general health, in northern China,eastern China, inland urban areas in southern China, and Taiwan; and the present study aimed to assess VA and its demographic and ocular correlations among several subgroups in detail in the population for which data have not been reported thus far. BCVA was statistical significantly associated with some sociodemographic factors, such as age, educational background, degree of urbanization, and geographic location.Sex was not statistical significantly correlated with BCVA considering the interdependence of the parameters with each other. According to our results, residents over 75y and residents with refractive error between 0.00 to -3.00 diopters had better presenting VA in urban area, while had no difference with BCVA between rural and urban areas, which may suggest the lower glasses use in mild myopia and in rural area.Presenting VA did not vary significantly between inland and coastal groups and BCVA was better in coastal group with nearly all age groups and with refractive error between 0.00 to+3.00 (0.89±0.220.83±0.25) and +3.00 to +5.00 (0.77±0.270.60±0.27) groups, which may revealed that residents with mild and moderate hyperopia in coastal area had better medical conditions and higher operation rate of cataract and other eye diseases, and paid more attention to the improvement of visual function. Presenting VA did not vary significantly between rural and urban groups and BCVA was better in urban group with all four education level groups, which revealed that urban residents had better medical conditions and higher operation rate of cataract and other eye diseases. Residents with middle and above education level had better BCVA in coastal area,while had no difference with presenting VA between inland and coastal areas. Residents with high income had no difference with presenting VA and BCVA between rural and urban and inland and coastal areas. All these results suggested residents with higher education level and higher income paid more attention to the improvement of visual function.

細胞內(nèi)蛋白的降解主要是通過泛素化過程進行。因此，CRM1的降解過程可能與細胞內(nèi)CRM1蛋白的泛素化增強有關(guān)。因此，本研究進一步通過激光共聚焦技術(shù)檢測CRM1的貨物蛋白RanBP1[19]的細胞核輸出情況。結(jié)果顯示，隨著LFS-01藥物濃度的增加，RanBP1在細胞核內(nèi)的聚集量逐漸增加（圖2顯示細胞核內(nèi)的綠色熒光逐漸增強），說明CRM1的細胞核輸出功能受到抑制。并且，20 μmol/L LFS-01抑制CRM1的細胞核轉(zhuǎn)運能力與50 nmol/L陽性對照藥物LMB[20]相當，約80%的RanBP1蛋白被滯留在細胞核內(nèi)（P值均＜0.01）。

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