紅茶水提物對小鼠肥胖及腸道微生物的影響

汪長鋼 賈紅亮 于佳弘 張佳霖

摘要 [目的]研究紅茶水提物對小鼠肥胖和腸道微生物的影響。[方法]根據體重相近原則，將實驗小鼠分為對照組（CK）、高脂模型組（HFD）、高脂+2.5%紅茶水提物（R2.5）、高脂+5%紅茶水提物（R5）、高脂+10%紅茶水提物（R10）5組，每組6只，喂養56 d。測定小鼠基礎指標和血清指標，并通過高通量測序技術研究小鼠腸道微生物菌屬相對豐度變化。[結果]不同劑量紅茶水提物處理小鼠的體重、體脂率、皮下脂肪率均降低，血清中TG、TC、LDL-C含量降低，且R5處理的效果顯著，加大劑量仍能取得相同效果。紅茶水提物處理后，小鼠的皮下脂肪率和肝重變化不明顯。LefSe分析結果顯示，肥胖模型小鼠 Akkermansia 菌屬豐度低于CK，且有害 菌Bilophila豐 度增加。結合3組小鼠腸道微生物分析，顯示 Parabacteroide菌 屬相對豐度隨著紅茶提取物劑量的上升逐漸下降， Prevotellaceae菌 屬相對豐度隨著紅茶水提物劑量的上升而上升。R5組小鼠 的Akkermansia菌 屬相對豐度顯著提高，隨著紅茶水提物劑量的提升， Akkermansia菌屬 相對豐度沒有顯著變化。[結論]紅茶水提物可以有效緩解小鼠肥胖，能顯著影響小鼠腸道 中Parabacteroide、Prevotellaceae、Akkermansia 的含量。

關鍵詞 紅茶水提物;肥胖;腸道微生物 ;Parabacteroide;Akkermansia

中圖分類號 TS 272.5+2? 文獻標識碼 A

文章編號 0517-6611（2021）19-0147-04

doi：10.3969/j.issn.0517-6611.2021.19.039

開放科學（資源服務）標識碼（OSID）：

Dose Effects of Black Tea Water Extract on Obesity and Intestinal Microbes in Mice

WANG Chang-gang1，JIA Hong-liang1，YU Jia-hong2? et al

（1.Beijing Agricultural Vocational College，Beijing 102442;2.Weihai Ocean Vocational and Technical College，Weihai，Shandong 264300）

Abstract [Objective]The effect of black tea water extract on obesity and intestinal microbes in mice was studied.[Method] According to the principle of similar body weight，the experimental mice were divided into 5 groups with 6 mice in each group. The five groups were treated separately，followed by the control group （CK），the high-fat model group （HFD），the high-fat+10.5% black tea water extract （R2.5），the high-fat +5.0% black tea water extract （R5），and the high-fat +10.0% black tea water extract （R10），feeding for 8 weeks. Determine the basic indicators and serum indicators of mice，and use high-throughput sequencing technology to study the relative abundance of mouse gut microbes.[Result]The results showed that the body weight，body fat percentage，and subcutaneous fat percentage of mice were reduced after treatment with different doses of black tea water extract，and the serum TG，TC，LDL-C content decreased，and the black tea water extract dose was 5% effective obviously，the same effect can still be obtained by increasing the dose. After the black tea water extract was treated，the subcutaneous fat rate and liver weight of the mice did not change significantly. The results of LefSe analysis showed that the abundance of ?Akkermansia ?in obese model mice was lower than that in control mice，and the abundance of harmful bacteria ?Bilophila ?increased. Combining the results of the intestinal microbial analysis of the three groups of mice，it was shown that the relative abundance of ?Parabacteroide ?gradually decreased with the increase of the black tea extract dosage，and the relative abundance of ?Prevotellaceae ?increased with the increase of the black tea water extract dosage. The results showed that the relative abundance of ?Akkermansia ?in mice in the R5 group was significantly increased，and the relative abundance of ?Akkermansia ?did not change significantly as the dose of black tea water extract increased. [Conclusion]Black tea water extract can effectively relieve obesity，and can significantly affect the contents of ?Parabacteroide，Prevotellaceae， and ?Akkermansia ?in the intestines of mice.