Majocchi's granuloma caused by Trichophyton rubrum after facial injection with hyaluronic acid:A case report

Jie Liu,Wen-Qiang Xin,Lan-Ting Liu,Chao-Feng Chen,Lin Wu,Xiao-Ping Hu

Jie Liu,Lan-Ting Liu,Chao-Feng Chen,Lin Wu,Xiao-Ping Hu,Department of Dermatology,Skin Research Institute of Peking University Shenzhen Hospital,Peking University Shenzhen Hospital,Shenzhen 518036,Guangdong Province,China

Wen-Qiang Xin,Department of Neurosurgery,Tianjin Medical University General Hospital,Tianjin 300052,China

Abstract

Key words:Trichophyton rubrum;Facial injection;Hyaluronic acid;Case report

INTRODUCTION

With the increasing popularity of cosmetic injections,such as hyaluronic acid and botulinum injection,an increasing number of related complications after cosmetic injections have been reported recently[1,2].Hyaluronic acid fillers may cause early and immediate adverse reactions,such as needle marks or pinpoint bleeding,erythema or redness,swelling and edema,pain,tenderness,and ecchymosis,in 0.05% to 2% of cases[3].Delayed adverse reactions,including hypersensitivity and granulomatous reaction,are reported to occur in up to 0.6% of cases[4].

Among the complications,the risk of infection increased by improper disinfection of the patient’s skin,incorrect injection techniques,decreased general immunity,and the presence of pathogens[5].To date,the difficulty in the study of Majocchi's granuloma(MG) from an infection lies in the accurate diagnosis of the organism and the identification of susceptibility to antifungal testingin vitro,which are important to guide the treatment of MG caused by pathogens.We report a case of MG caused byTrichophyton rubruminfection after facial injection of hyaluronic acid.

CASE PRESENTATION

Chief complaints

A 37-year-old woman presented with red papules,nodules,and abscesses on her left zygomatic arch for 2 mo.

History of present illness

The patient’s symptoms started 2 mo prior to evaluation.The scale and size of the skin lesions on her face gradually increased,and some of the nodules and plaques ruptured and caused pain.

History of past illness

The patient received cosmetic injection of hyaluronic acid on the left side of her face in a small clinic prior to the appearance of lesions.The patient was diagnosed with a skin infection at the local hospital,but the lesions did not improve after antibiotic ointment treatment.She had no history of fungal infections or other systemic diseases,no history of risk from her residence,no contact with pets,and no history of tuberculosis.

Physical examination

Dermatological examination revealed red papules,nodules,and abscesses on the left side of her face.The size of the nodules varied,the largest nodule was approximately 0.8 cm × 0.6 cm and the smallest nodule was approximately 0.3 cm × 0.2 cm.Some nodules and abscesses produced purulent secretions.Necrotic tissue was attached to the wound,causing burning,pinching pain in the patient (Figure 1A).

Laboratory examinations

Laboratory examinations revealed a leukocyte count of 4 × 109/L,hemoglobin level of 110 g/L,and platelet count of 170 × 109/L.There were no abnormal results in the renal and hepatic function tests.Tests for hepatitis B,hepatitis C,human immunodeficiency virus,cytomegalovirus and Epstein-Barr virus were negative.Histological examination revealed diffuse multinucleated giant cells as well as infiltration of lymphocytes and neutrophils into the dermis.The pathological findings of the skin biopsy included acanthosis and papilloma by hematoxylin-eosin staining (Figure 2A)and dermal granuloma and cytoplasmic branched septate hyphae by periodic acid-Schiff staining (Figure 2B).The results of the T-SPOT-TB test,acid-fast stain,human immunodeficiency virus test,and rapid plasma reagin test were also negative.

Mycology examinations

Skin biopsy samples taken from the active borders were cultured inSabouraud dextroseagar and incubated at 28 °C for 2 wk.Septated hyaline hyphae and chains of arthrospores were visualized on 10% KOH wet mount preparations.The organism grew radially and produced reddish-brown and yellow pigments at the same time(Figure 3).We further extracted the fungal deoxyribonucleic acid and employed primersITS-1(5’-TCCGTAGGTGAACCTGCGG-3’) andITS-4(5’-TCCTCCGCTTATTGATATGC-3’) to perform polymerase chain reaction amplification.The amplification product was sequenced and then analyzed with BLAST in GenBank,and the genetic sequence was more than 99% similar to the standard strain ofTrichophyton rubrumCBS 189.69 (Figure 4).Therefore,the fungus was finally identified asTrichophyton rubrum.We performedin vitroantifungal susceptibility testing according to the Clinical and Laboratory Standards Institute M38-A2 method[6]and found that the isolated strain ofTrichophyton rubrumwas sensitive to most antifungal agents,including terbinafine (<0.5 μg/mL),itraconazole (0.06 μg/mL),amphotericin B (0.25 μg/mL),fluconazole (32 μg/mL),voriconazole (0.125 μg/mL),posaconazole(0.125 μg/mL),and isavuconazole (0.06 μg/mL).

FINAL DIAGNOSIS

A diagnosis of MG caused byTrichophyton rubrumafter facial injection of hyaluronic acid was made based on the cosmetic injection history,the presence of skin lesions,morphological and molecular biological results and histological examination.

TREATMENT

The patient was initially started on terbinafine 250 mg/d for 2 mo,but the painful papules,nodules and abscesses on her face did not improve.Then,we adjusted the treatment to itraconazole 400 mg/d for 8 wk based on thein vitroantifungal susceptibility testing results.

OUTCOME AND FOLLOW-UP

The skin lesions improved significantly after treatment with itraconazole 400 mg/d for 8 wk,and there was no recurrence during the follow-up (Figure 1B).

DISCUSSION

Facial cosmetic procedures become popular because of people’s desire for a younger appearance and the development of cosmetic technology over the past severaldecades[7].To date,hyaluronic acid is reported to be safe and well tolerated and has become one of the most important fillers for cosmetic rejuvenescence.However,complications,such as infection,anaphylaxis and embolism after facial injection,are more common as more cosmetic procedures are performed,especially cosmetic injections administered in small unlicensed clinics.

Figure 1 Physical examination.A:Before treatment;B:After oral itraconazole for 8 wk.

Figure 2 Histologic examination of the skin lesion demonstrated diffuse suppurative and granulomatous inflammation.The diffuse infiltration of lymphocytes,histiocytes,and multinucleated giant cells was observed in the dermis.A:Fungal mycelium was clearly observed in a giant cell(Hematoxylin-eosin staining,× 400);B:Several giant cells and macrophages were stained with periodic acid-Schiff (Hematoxylin-eosin staining,× 1000).

Figure 3 Mycology examinations.A white and dark red colony was observed.

Dermatophytes are widespread and are among the most common cause of superficial fungal infections worldwide[8].Trichophyton rubrumis currently recognized as the most common fungal pathogen among dermatophyte infections and is often intractable.MG is an inflammatory and granulomatous dermatophyte infection,and the main pathogen that causes MG isTrichophyton rubrum.It has been reported that the number of cases of MG has been rising over the years[9].In the present case,the patient and her family members had no history of dermatophytosis.She had no contact withothers except her family members after undergoing cosmetic injection of hyaluronic acid.She presented with multiple painful abscesses on the left side of her face,and the lesion was located at the site of cosmetic injection.A contaminant from the operating room of the unqualified clinic where the injection procedure was performed likely caused theTrichophyton rubruminfection.

Figure 4 Deoxyribonucleic acid sequence alignment of isolated Trichophyton rubrum and Centraalbureau voor Schimmelcultures 189.69.CBS:Centraalbureau voor Schimmelcultures.

Systemic treatment ofTrichophyton rubruminfection mainly includes allylamines,azoles,polyenes and hydroxypyridones.In general,terbinafine (250 mg/d),as a representative allylamine antifungal agent,is the preferred systemic antifungal drug for the treatment of MG and is used in 40.8% of cases.When the infectious agent cannot be isolated,terbinafine should be the first-choice treatment because organisms resistant to itraconazole or griseofulvin have been reported to be responsive to terbinafine.Wanget al[10]presented two cases of MG with different clinical features.Both patients had satisfactory relief after treatment with terbinafine.Suet al[11]also successfully treated a case ofTrichophyton rubruminfection characterized by MG and deeper dermatophytosis with oral terbinafine 250 mg/d,and the patient's skin lesion disappeared.However,terbinafine-resistant strains have been found in cases of drugresistant dermatophyte infection,includingTrichophyton rubruminfection,in recent years[12-14].Other systemic antifungal drugs to treat dermatophyte infections include itraconazole,griseofulvin,ketoconazole,voriconazole,and posaconazole[15-19].Guptaet al[20]found that two pulses of oral itraconazole therapy appeared to be effective in the treatment of MG.In the case we reported here,the patient was unresponsive to terbinafine and was successfully treated with itraconazole,which should indicate the need for doctors to select treatment agents by performingin vitroantifungal susceptibility testing.

Mycology examinations are important to identify the causative agent,especially the type of invasive dermatophytosis,and can determine the treatment.In the case of MG and other invasive dermatophyte infections that are difficult to treat,such as mycetoma and pseudomycetoma,terbinafine is the preferred choice for treatment.Therefore,it is of key importance to accurately diagnose the disease-causing agents,especially in immunocompromised patients.In addition,cosmetic injections should be given in a sterile environment,and people should be aware of the risk of fungal infection in the pursuit of beauty.

CONCLUSION

This case revealed that facial injection of hyaluronic acid may cause serious MG.Antifungal susceptibility testing should be conducted to help select proper agents during clinical treatment.