基于轉錄組的劍麻ARF基因家族的鑒定及表達分析

鹿志偉 侯曉婉 楊子平 張燕梅 李俊峰 周文釗

摘 ?要??生長素反應因子（Auxin Response Factors， ARFs）是植物特有的多基因轉錄因子家族，參與植物生長發育過程的調節。本研究基于轉錄組數據，通過生物信息學方法鑒定劍麻ARF基因家族成員，同時對其進行相關生物信息學分析。結果表明：在劍麻中共鑒定得到15個ARF基因，并依次命名為AhARF1～15。其編碼的蛋白質含有409～1011個氨基酸，分子量約為45.17～112.13?ku，等電點為5.17～9.34。亞細胞定位預測結果顯示，13個AhARFs定位于細胞核，2個AhARFs定位于葉綠體。保守結構域分析結果表明，AhARFs基因家族有8個成員同時含有B3、ARF和Aux/IAA這3個相對保守的結構域，其他成員僅含有B3和ARF這2個結構域。進化樹分析表明，劍麻AhARFs蛋白可分為5個亞家族。15個劍麻AhARFs基因在劍麻紫色卷葉病不同發病時期呈現不同的表達規律。本研究為進一步深入探索劍麻ARF基因家族的功能奠定了重要理論基礎。

關鍵詞 ?劍麻;ARF;基因家族;轉錄組中圖分類號??S563.8??????文獻標識碼??A

Transcriptome-wide Identification and Expression Profiling of the Auxin Response Factor （ARF） Gene Family in Agave.hybrid?No.11648

LU Zhiwei^1，2，?HOU Xiaowan¹，?YANG Ziping^1，2，?ZHANG Yanmei^1，2，?LI Junfeng^1，2，?ZHOU Wenzhao^1，2*

1.?South Subtropical Crops Research Institute， Chinese Academy of Tropical Agricultural Sciences， Zhanjiang， Guangdong?524091， China; 2.?Zhanjiang City Key Laboratory for Tropical Crops Genetic Improvement，?Zhanjiang， Guangdong?524091， China

Abstract ?Auxin response factors （ARFs） are plant-specific polygenic transcription factor families involved in the regulation of plant growth and development. In this study， the members of the agaveARF gene family were identified and analyzed by bioinformatics methods， based on transcriptome data. The results showed that 15ARFgenes were identified in agave and named fromAhARF1toAhARF 15in turn， its encoded protein varied from 409 to 1011 amino acid residues in length， the molecular weight ranged from 45.17 to 112.13 ku， and the isoelectric point varied from 5.17 to 9.34. Subcellular localization prediction showed that 13AhARFswere localized in the nucleus and 2AhARFswere located in the chloroplast. Conservative domain analysis results indicated that there were eight members among theAhARFsgene family which containing three relatively conserved domains， B3， ARF， and Aux/IAA. The other members contained only the two domains， B3 and ARF. Phylogenetic tree analysis indicated that the AhARFs protein could be divided into five subfamilies. The 15AhARFsgenes showed different expression patterns in different stages of agave purple leaf curl disease. This research would lay an important theoretical foundation for further study of the function of theAhARFsgene family.

Keywords ?agave;ARF;?gene family; transcriptome

DOI10.3969/j.issn.1000-2561.2019.10.09

生長素響應因子（auxin response factor， ARF）是一種調控生長素響應基因表達的轉錄因子，廣泛存在于植物中，在植物生長發育過程的各個階段中起著重要調控作用^[1-2]。所有的ARF蛋白均含有一個保守的N端B3 domain，一個Auxin-resp domain（ARF）^[3]。前者是DNA結合結構域，負責與靶基因結合。后者是ARF的功能結構域，能夠在外界信號刺激下特異性地與靶基因啟動子中的生長素響應元件--TGTCTC結合，激活或者抑制靶基因的表達，進而行使對植物生長發育的調控功能^[4-5]。此外，有些ARF蛋白質C末端還含有Aux/IAA二聚化結構域，能夠通過同源或異源二聚體的形成來調節ARF蛋白質的活性^[6-7]。……