菠蘿R2R3-MYB基因家族鑒定與表達分析

陳哲 胡福初 阮城城 范鴻雁 郭利軍 張治禮

摘 ?要??R2R3-MYB轉錄因子參與植物眾多生命活動的調控，在植物生長發育中至關重要。本研究采用生物信息學分析方法，從菠蘿基因組中篩選鑒定R2R3-AcMYB轉錄因子，并對其基因結構、編碼蛋白和系統進化進行了分析;基于轉錄組數據和熒光定量PCR分析，研究了乙烯利處理后菠蘿頂端分生組織MYB基因的表達模式。研究結果顯示，菠蘿基因組含有103個R2R3-AcMYB轉錄因子，其中67個的基因結構組成為3（外顯子）+2（內含子），17個為2（外顯子）+1（內含子）。103個R2R3-AcMYB蛋白中，有31個偏堿性，55個顯酸性和17個呈中性。亞細胞定位預測顯示，有67個編碼蛋白定位于細胞質，20個定位于細胞核。保守基序分析發現，有91個R2R3-AcMYB的序列包含SANT結構的motif 1和motif 2。系統進化樹分析表明，81個R2R3-AcMYB轉錄因子可以分別被歸入18個亞組，其余22個R2R3-AcMYB轉錄因子則未能進行明確歸類。基于轉錄組數據和熒光定量PCR分析結果，發現菠蘿頂端分生組織中多個MYB基因的表達受到乙烯利的誘導或抑制，暗示這些基因可能參與了乙烯利誘導條件下菠蘿生長發育（包括開花誘導等）調控。這些研究結果為進一步挖掘菠蘿激素響應、生長發育和開花調控等基因，揭示菠蘿生長發育調控機制提供了數據支持。

關鍵詞 ?菠蘿;R2R3-MYB;生物信息學;乙烯利;基因表達中圖分類號??S668.3??????文獻標識碼??A

Bioinformatics and Gene Expression Analysis of Pineapple R2R3-MYB Gene Family

CHEN Zhe， HU Fuchu， RUAN Chengcheng， FAN Hongyan， GUO Lijun， ZHANG Zhili^*

Institute of Tropical Fruit Trees， Hainan Academy of Agricultural Sciences?/ Key Laboratory of Tropical Fruit Tree Biology of Hainan Province / Haikou Investigation Station of Tropical Fruit Trees， Ministry of Agriculture?and?Rural?Affairs， Haikou， Hainan 571100， China

Abstract ?R2R3-MYB transcription factors?are very important in plant growth and development， ich are involved in the regulation of many life activities of plants. In this study， the R2R3-AcMYB transcription factors were screened and identified from the pineapple genome by bioinformatics analysis. Based on the transcriptome data?and fluorescence-quantitative PCR analysis， the expression patterns of MYB genes in the apical meristem of pineapple treated with ethylene were studied. The results showed?that there were?103 R2R3-AcMYB transcription factors in the pineapple genome， of which 67 gene were?composed of 3?（exons） +2?（introns） and 17 were?2?（exons） +1?（introns）. Of the 103 R2R3-AcMYB proteins， 31 were?alkaline， 55 were?acidic and 17 were?neutral， and the main secondary structure in 83 proteins was random curl. Subcellular localization predictions showed that 67 proteins were?located in the cytoplasm and 20 in the nucleus. Conservative motif analysis found that 91 of the R2R3-AcMYB sequences contained motif 1 and motif 2 of the SANT structure. Phylogenetic analysis showed?that 81 R2R3-AcMYB transcription factors could?be classified into 18 subgroups， and the remaining 22 R2R3-AcMYB transcription factors could?not be clearly classified. Based on transcriptome data and fluorescence-quantitative PCR analysis， it was found that the expression of several MYB genes in the apical meristem of pineapple was induced or inhibited by ethrel， suggesting that the genes may be involved in the responses of pineapple growth and development including the induction of flowering?to ethrel. The results would provide data supports for further excavation of genes involved in hormone response， growth and development，?and flowering regulation， which would?contribute to revealing the regulation mechanism of pineapple growth and development.

Keywords ?pineapple; R2R3-MYB; bioinformatics; ethrel; gene expression

DOI10.3969/j.issn.1000-2561.2019.10.008

MYB類轉錄因子家族是指含有MYB結構域的一類轉錄因子，是真核生物中存在的最大的一類轉錄因子家族。典型的MYB類轉錄因子一般由DNA結合結構域、1個轉錄激活結構域和1個不完全界定的負調節區3個保守功能域組成^[1-2]。……