龍眼類受體蛋白激酶CRK家族全基因組鑒定及表達調控分析

孫瑩 林藝靈 趙鵬程 徐小萍 陳曉慧 申序 林玉玲 賴鐘雄

摘 ?要??為了解龍眼富含半胱氨酸的類受體蛋白激酶（cysteine-rich receptor-like kinase，CRK）家族的基本特征與功能，本研究采用生物信息學分析方法對篩選出的98個龍眼CRK基因家族的成員進行分析鑒定，包括分子進化樹的構建和對蛋白結構域及其特性、啟動子及其順式作用元件、體胚發生的過程以及組織器官特異性表達的分析。結果顯示：通過對龍眼和擬南芥CRK基因做進化樹分析，可根據親緣關系的遠近將其分為7個亞家族;不同亞家族成員的蛋白質特性（氨基酸數量、相對分子質量、等電點、信號肽及糖基化位點）有所不同;各成員基因結構特性，啟動子順式作用元件，蛋白結構域特性等與進化樹中家族成員親緣關系的遠近相關;98個CRK家族成員在體胚發生過程（NEC、EC、ICpEC、GE）以及生長發育過程中組織器官特異性表達情況有所不同，其中在非胚性愈傷組織以及幼果中表達量較高，在種子和果實中幾乎未表達。此外，富含半胱氨酸的類受體蛋白激酶在生物脅迫、非生物脅迫（抗病蟲害、抗旱、抗寒、抗鹽脅迫等）以及在植物生長發育過程中起著重要作用。

關鍵詞 ?龍眼;CRK;基因家族;鑒定;表達調控中圖分類號??S667.2??????文獻標識碼??A

Genome-wide Identification and Regulation of Expression Analysis of Cysteine-rich Receptor-like Kinase in Longan

SUN?Ying， LIN Yiling，?ZHAO Pengcheng， XU?Xiaoping， CHEN Xiaohui， SHEN Xu， LIN Yuling，LAI Zhongxiong^*

Institute of Horticultural Biotechnology， Fujian Agriculture and Forestry University， Fuzhou， Fujian?350002， China

Abstract ?In order to understand the basic characteristics and functions of cysteine-rich receptor-like kinase （CRK） family in longan， a series of bioinformatics methods were used to analyze and identify 98 members ofCRKgene family?members， including constructing the molecular evolutionary tree， analyzing protein domains and characteristics， promoters， homeopathic elements， somatic embryogenesis and tissue-organ specific expression. Results showed?that the?CRK gene of longan andArabidopsiscould be divided into seven subfamilies according to the distance of the relationship by evolutionary tree analysis， and the protein characteristics （the quantity?of amino acids， the relative molecular weight， the isoelectric point， the signal peptide and sites?of glycosylation） of different subfamilies were?different. Structural characteristics of the genes?of each member， promoter homeopathic elements and protein domain characteristics were?closely related to family members in the evolutionary tree.?The specific expression of 98?CRK family members during somatic embryogenesis （NEC， EC， ICpEC， GE） and growth were different， particularly higher in non-embryogenic callus and young fruits， but they were?almost not expressed in seeds and matured?fruits. In addition， cysteine-rich receptor-like protein kinases?played?important roles?in biological stress， abiotic stress （insect-induce diseases， drought resistance， cold resistance， salt resistance， etc.） and it played?important roles?in plant growth and development as well.

Keywords ?longan;CRK;?gene family; identification; regulation of expression

DOI10.3969/j.issn.1000-2561.2019.10.005

富含半胱氨酸類受體蛋白激酶（cysteinerich receptor-like kinase， CRK）在植物類受體蛋白激酶（receptor-like kinase， RLK）家族中具有重要地位，在植物生長發育、激素信號傳導、生物和非生物脅迫以及植物抗病性細胞過敏性死亡中發揮著至關重要的作用^[1-2]。

國內外研究發現CRK家族基因可調控植物生長發育及抵抗逆境。……