龍眼HD-Zip基因家族生物信息及表達分析

張春渝 徐小萍 陳曉慧 申序 林玉玲 賴鐘雄

摘 ?要??為了解龍眼HD-Zip基因家族的功能，本研究基于龍眼基因組與轉錄組數據庫，對提取的19個龍眼HD-Zip家族成員的啟動子、可變剪接以及受到調控的miRNA種類進行分析;并采用實時熒光定量PCR技術，分析了在ABA處理下龍眼胚性愈傷組織（embryonic callus，EC）中HD-Zip部分成員的表達模式，以及HD-Zip部分成員在不同體胚階段的表達模式。結果表明：龍眼HD-Zip家族除含有TATA-box和CAAT-box以外還含有大量的光響應元件、激素響應元件以及脅迫響應元件，暗示龍眼HD-Zip家族成員可能參與光反應、激素響應以及非生物脅迫的過程;龍眼HD-Zip在不同組織部位與不同體胚階段的可變剪接方式主要以內含子保留為主;龍眼HD-Zip基因家族成員主要受到miRNA166a的調控;在外源激素ABA處理下龍眼EC中HD-Zip家族部分成員的表達模式以及部分成員在不同體胚階段的表達模式都呈現多樣化，推測龍眼HD-Zip參與龍眼不同體胚發育的調控，并且可能通過調節內源ABA的濃度進而影響胚性愈傷組織的生長發育以及形態的建成。

關鍵詞 ?龍眼;HD-Zip基因;體胚;表達模式中圖分類號??S667.2??????文獻標識碼??A

Biological Information and Expression Analysis of HD-ZipGene Family in Dimocarpus longan?Lour.

ZHANG Chunyu， XU Xiaoping，?CHEN Xiaohui，?SHEN Xu，?LIN Yuling， LAI Zhongxiong^*

Institute of Horticultural Biotechnology， Fujian Agriculture and Forestry University， Fuzhou， Fujian 350002， China

Abstract ?In order to understand the function of the longanHD-Zipgene family， this study was based on the longan genome and transcriptome database， and analyzed the promoters， alternative splicing and regulated miRNA species of 19 longanHD-Zipfamily members， and the expression pattern ofHD-Zipmembers in longan embryogenic callus under ABA treatment and the expression patterns of some members at different somatic stages was analyzed by real-time fluorescent quantitative PCR. The longanHD-Zipfamily contained a large number of photoresponsive elements， hormone response elements and stress response elements in addition to TATA-box and CAAT-box， suggesting that longan?HD-Zipfamily members may be involved in photoreactivity， hormone response and non-biological stress. The alternative splicing mode of longanHD-Zipin different tissue parts and different somatic embryo stages was mainly intron retention. The longanHD-Zipgene family members were mainly regulated by miRNA166a. The expression patterns of some members of theHD-Zip family in the longan EC stage under the treatment of exogenous hormone ABA?and the expression patterns of some members in different somatic embryo stages were diverse. It is speculated that longanHD-Zipparticipates in the regulation of different somatic embryo development in longan， and may affect the growth and development of embryogenic callus and the formation of morphology by regulating the concentration of endogenous ABA.

Keywords ?Dimocarpus longan Lour.;HD-Zipgene;?somatic embryo;?expression mode

DOI10.3969/j.issn.1000-2561.2019.10.004

HD-Zip（同源異型-亮氨酸拉鏈）蛋白是植物中特有的一類轉錄因子，屬于同源異型盒（homeobox，HB）蛋白家族。它包含有一個高度保守的同源異型結構域（HD）和一個亮氨酸拉鏈（LZ）結構域。HD-Zip蛋白可以根據結合的特異性DNA序列、基因結構、包含的其他基序以及功能等4個方面，分成4個不同的亞家族^[1-2]。近年來，HD-Zip已經在多種植物中有所報道，如大豆^[3]、桃^[4]、番茄^[5]等。HD-Zip基……