亞香棒蟲草多糖C-10體外抗腫瘤活性研究

鄧贛奇 劉燊 冷桂華

摘要 [目的]研究亞香棒蟲草多糖C-10體外抗腫瘤活性。[方法]以亞香棒蟲草分離篩選的內(nèi)生真菌為菌種，液體轉(zhuǎn)化發(fā)酵產(chǎn)蟲草多糖，經(jīng)過分離提取得胞內(nèi)和胞外多糖，并探討其對3株癌細(xì)胞（肺腺癌A549細(xì)胞、乳腺癌MCF-7細(xì)胞、神經(jīng)母癌SH-SY5Y細(xì)胞）的生長抑制效果。[結(jié)果]隨著時間和濃度的增大，胞內(nèi)多糖對癌細(xì)胞的抑制率逐漸增大，且差異較為明顯，在培養(yǎng)72 h的條件下，BN-H多糖（0.096 6 mg/mL）對肺腺癌A549細(xì)胞、乳腺癌MCF-7細(xì)胞、神經(jīng)母癌SH-SY5Y細(xì)胞的抑制率分別為97%、71%、97%，比其他濃度的胞內(nèi)外多糖抑制效果更好。[結(jié)論]BN-H（0.096 6 ?mg/mL）的亞香棒蟲草多糖對肺腺癌、乳腺癌和神經(jīng)母癌細(xì)胞具有極顯著抑制作用（P<0.01），具有較強(qiáng)的時間和濃度依賴性，且胞內(nèi)多糖對癌細(xì)胞的抑制效果好于胞外多糖。

關(guān)鍵詞 亞香棒蟲草;蟲草多糖;癌細(xì)胞;MTT法;抗腫瘤活性;抑制率

中圖分類號 S 567.3+5 ?文獻(xiàn)標(biāo)識碼 A

文章編號 0517-6611（2019）12-0187-04

doi：10.3969/j.issn.0517-6611.2019.12.051

開放科學(xué)（資源服務(wù)）標(biāo)識碼（OSID）：

Abstract [Objective] The research aimed to ?study the antitumor activity of Cordyceps hawkesii polysaccharide C10 in vitro.[Method]The endophytic fungi isolated from Cordyceps hawkesii were used as strains to produce cordyceps polysaccharides by liquid transformation.The intracellular and extracellular polysaccharides were isolated and extracted，and the inhibitory effects on the growth of three cancer cells （A549 cells，breast cancer MCF7 cells and neuroblastoma cancer SHSY5Y cells） were investigated.[Result]With the increase of time and concentration，the inhibition rate of intracellular polysaccharides on cancer cells was gradually increased，and the difference was obvious.Under the condition of raising 72h，the inhibitory rates of cancer A549 cells，breast cancer MCF7 cells and neuroblastoma cancer SHSY5Y cells were 97%，71% and 97% respectively，which were better than those of other concentrations of extracellular polysaccharides.[Conclusion]The polysaccharide of BNH （0.096 6 ?mg/mL） can inhibit lung adenocarcinoma，breast cancer and neuroblastoma significantly （P<0.01），and has a strong time and concentration dependent effect on lung adenocarcinoma，breast cancer and neuroblastoma （P<0.01）.The inhibitory effect of intracellular polysaccharides on cancer cells was better than that on extracellular polysaccharides.

Key words Cordyceps hawkesii;Cordyceps polysaccharide;Cancer cell;MTT method;Antitumor activity;Inhibition rate

蟲草主要由兩部分構(gòu)成，即千尸毛“蟲”和頭部長出來的真菌“草”，它的各種活性成分物質(zhì)具有降壓[1]、舒張血管[2]、調(diào)節(jié)免疫系統(tǒng)功能[3]、抗腫瘤[4]、抗菌[5]、抗氧化[6]等功效[7]。冬蟲夏草被稱為我國的“中藥之王”，位列三大補(bǔ)品之首，研究發(fā)現(xiàn)，在中國境內(nèi)有100多種蟲草，只有一種叫做冬蟲夏草，足見其稀缺珍貴[8]。亞香棒蟲草（Cordyceps hawkesii Gray.） 又稱霍克斯蟲草，蟲體外面灰黃色，“草部”呈淡灰色至灰黑色。亞香棒蟲草比較脆而且容易折斷，氣微香，味淡，其主要靠寄生鱗翅目蝙蝠蛾科棒蝠蛾屬（Napialus）幼蟲來生長[9]。在成分和功能作用上與冬蟲夏草十分接近，因此有些地方直接用亞香棒蟲草代替冬蟲夏草食用，具有止咳、化痰、定喘、安神、益精氣及益腎壯陽等功效[10]。……