磁性納米Fe₃O₄對黃鯽蛋白抗菌肽的富集作用

冀雪莉 韋榮編 宋茹

摘要 [目的]以磁性Fe3O4粒子為基粒，采用溶膠-凝膠法在基粒表面鍵合二氧化硅，將基粒制成改性磁性納米Fe3O4用于吸附黃鯽蛋白抗菌肽中的活性肽，從而達到對黃鯽蛋白肽中有效抗菌組分的快速分離富集。[方法]黃鯽蛋白肽經改性磁性納米Fe3O4吸附后，測定抑菌率、肽含量、蛋白質含量和氨基酸含量指標，再結合透射電鏡分析和高效液相分析結果，比較黃鯽蛋白肽在吸附前后的變化。[結果]透射電鏡結果顯示，改性磁性納米Fe3O4吸附黃鯽蛋白抗菌肽，可在粒子表面形成“冕層”。經改性磁性納米Fe3O4吸附后，黃鯽蛋白抗菌肽液對大腸桿菌的抑菌作用顯著降低，其中黃鯽蛋白肽中的肽含量、蛋白質含量分別由吸附前的7.249 mg和416.221 μg降低至6.297 mg和346.183 μg，而吸附后肽液中氨基酸含量則略有提高。高效液相分析結果表明，黃鯽蛋白肽經改性磁性納米Fe3O4吸附后主要分離峰的保留時間和峰面積均發生改變。[結論]改性磁性納米Fe3O4能有效吸附黃鯽蛋白肽中的抗菌肽，實現對抗菌組分的分離富集作用。

關鍵詞 黃鯽蛋白肽;抗菌;磁性Fe3O4粒子;表面修飾;富集

中圖分類號 TS254.4文獻標識碼 A

文章編號 0517-6611（2019）10-0141-03

Abstract [Objective]The magnetic Fe3O4 particles was modified by combining the silica layer using the solgel method，with the aim of enrichment of antibacterial peptide from halffin anchovy hydrolysates （HAHp）.[Method]Determine the inhibition rate，peptide content，protein content and amino acid content of absorbed HAHp，and combine the results of transmission electron microscopy and high performance liquid phase analysis to compare the changes of HAHp before and after adsorption.[Result]Transmission electron microscopy showed that a coronal layer was formed on the surface of modified magnetic Fe3O4 after treated with HAHp.The antibacterial activity of HAHp was significantly reduced after adsorption by modified magnetic particles.The amounts of peptide and protein in HAHp were decreased from 7.249 mg to 6.297 mg，and 416.221 μg to 346.183 μg，respectively.By comparison，the amino acid content of HAHp was slightly increased after modified magnetic particles adsorption.Furthermore，the result of highperformance liquid phase analysis showed the changes of retention time and peak area for major peaks in HAHp after adsorbed by modified magnetic particles.[Conclusion]Our results suggested that the modified magnetic Fe3O4 particles could effectively enrich the antibacterial peptide from HAHp.

Key words Halffin anchovy peptides;Antibacterial activity;Magnetic Fe3O4 particles;Surface modification;Enrichment

黃鯽（Setipinna taty）屬于輻鰭魚綱、鯡形目、鳀科、黃鯽屬，在我國東海、南海、渤海等海域可以常年捕獲，且捕獲量較大[1]。通過酶解法制備的黃鯽蛋白肽除了抗菌活性外，還具有抗氧化、抑制腫瘤細胞增殖等生物活性[2-5]。前期研究中發現黃鯽蛋白肽的抑菌活性與其所帶電荷性有關[2]。磁性納米材料通常是由超順納米磁性粒子與無機或有機分子通過包覆或交聯等方式形成，可均勻分散于溶液中保持較高穩定性。……