mdig基因在人胰腺癌中的表達及其促癌作用

羅和生+占婷+田霞+黃曉東

[摘要] 目的 研究mdig基因在胰腺癌細胞中的表達情況，探討mdig與胰腺癌發生發展的關系及其作用的可能機制。 方法 采用實時熒光定量聚合酶鏈反應（Q-PCR）和Western blot方法檢測mdig在胰腺癌細胞PANC1和MIAPaca2與正常胰腺導管上皮細胞HPDE6c7中表達的差異。將轉染mdigsi RNA質粒的胰腺癌細胞PANC1和MIAPaca2設為實驗組，將轉染空載質粒的胰腺癌細胞PANC1和MIAPaca2設為對照組，用CCK8法和流式測凋亡法分別檢測兩組細胞的增殖和凋亡情況；用Western blot檢測兩組細胞的Sox2基因表達情況。 結果 與正常胰腺導管上皮細胞HPDE6c7相比，mdig基因在胰腺癌細胞PANC1和MIAPaca2中表達均上調（P < 0.05）。與對照組相比，實驗組細胞的增殖率下降（P < 0.05），凋亡率增高（P < 0.05），Sox2的表達下調（P < 0.05）。 結論 mdig在胰腺癌細胞中表達升高，并可能通過介導原癌基因Sox2發揮促癌作用。

[關鍵詞] 胰腺癌；礦物粉塵誘導基因；增殖；凋亡

[中圖分類號] R735 [文獻標識碼] A [文章編號] 1673-7210（2017）03（b）-0012-04

Expression of mdig gene in human pancreatic carcinoma and its role in promoting cancer

LUO Hesheng1 ZHAN Ting1 TIAN Xia HUANG Xiaodong2

1.Department of Gastroenterology， People's Hospitial of Wuhan University， Hubei Province， Wuhan 430060， China； 2. Department of Gastroenterology， Tongren Hospital of Wuhan Univerity， Hubei Province， Wuhan 430060， China

[Abstract] Objective To study the expression of mdig in pancreatic cancer cells， in order to explore the relationship between mdig and the development of pancreatic cancer and its possible mechanism. Methods The expression of mdig in pancreatic cancer cells and normal pancreatic ductal epithelial cells were detected by Q-PCR and Western blot. The pancreatic cancer cells PANC1 and MIAPaca2 transfected with mdigsi RNA plasmid were designated as the experimental group， and the pancreatic cancer cells PANC1 and MIAPaca2 transfected with empty plasmid were designated as control group， the proliferation of two groups of cells was detected by CCK8， the apoptosis of two groups of cells was detected by flow cytometry， the expression of Sox2 in two groups of cells was detected Western blot. Results Compared with normal pancreatic ductal epithelial cells HPDE6c7， the expression of mdigin pancreatic cancer cells PANC1 and MIAPaca2 was up-regulatede. Compared with the control group， the proliferation rate of the experimental group was decreased （P < 0.05）， the apoptosis rate was increased （P < 0.05）， and the expression of Sox2 was down regulated （P < 0.05）. Conclusion The expression of mdig in pancreatic cancer cells increases， and mdig may regulate the expression of Sox2 to promoting the occurrence of pancreatic cancer.

[Key words] Pancreatic cancer； Mdig； Proliferation； Apoptosis

近年來，胰腺癌的發生率和死亡率逐漸升高，已成為癌癥致死的第4位病因[1]，它的侵襲性生長和早期轉移擴散使其成為最具侵襲性的腫瘤之一，約40%患者在確診時已經發生局部或遠處轉移，失去根治性手術的機會[2]。隨著研究的深入發現，腫瘤的發生往往是多種基因共同作用的結果[3-4]因此可以通過研究與胰腺癌發病密切相關的基因，從而在分子水平上尋找其中的關鍵基因并開發相應的靶向治療。礦物粉塵誘導基因（mineral dust induced gene，mdig）于2005年作為一種新肺癌基因被發現[5]，隨后被證實與mina53基因是同一種基因，而mina53基因是原癌基因c-myc的靶基因[6]。越來越多的研究證實mdig基因是一種重要的腫瘤相關基因，與某些腫瘤的發生與發展密切相關，并且已經有報道發現mdig在人類結腸癌[7]，食管鱗狀細胞癌[8]、肝細胞癌[9]、膽管細胞癌[10]表達均升高。然而，目前只有極少數關于mdig基因在胰腺癌組織中的表達情況的報道，而且其表達情況的定量研究及其與胰腺癌發生發展的關系還未見報道。本研究擬探討mdig基因在胰腺癌細胞中表達狀況及其促癌作用并探討其發揮作用的可能機制。