銅仁市萬(wàn)山區(qū)野生鞣料植物資源調(diào)查

摘要：本文運(yùn)用生產(chǎn)中常用的恢復(fù)系蜀恢498為父本，貴州大學(xué)水稻研究所培育的貴紅一號(hào)為母本構(gòu)建的F4 群體為材料，對(duì)水稻紅米色素基因進(jìn)行定位。經(jīng)過(guò)經(jīng)典遺傳學(xué)分析確定了本實(shí)驗(yàn)材料的紅米色素是由一對(duì)主效基因控制，其它微效基因具有修飾作用。運(yùn)用基因池法篩選出12對(duì)具有多態(tài)性的SSR引物，分別位于第2、第4、第5、第6、第7和第10染色體上。運(yùn)用mapmaker 30等定位軟件，共檢測(cè)到2個(gè)QTL位點(diǎn)，位于RM258-RM228的qAN-10a和位于RM5414-RM252的qAN-4a。其中qAN-10a在錦屏和惠水2個(gè)地點(diǎn)均檢測(cè)到，具有良好的遺傳穩(wěn)定性，且貢獻(xiàn)率較大，認(rèn)為其為主效QTL，可為后續(xù)的精細(xì)定位提供參考。

關(guān)鍵詞：水稻；花色苷；QTL

中圖分類號(hào)：Q9581；S4433（273）

文獻(xiàn)標(biāo)識(shí)碼：A

文章編號(hào)：1008-0457（2017）03-0075-03國(guó)際DOI編碼：10.15958/j.cnki.sdnyswxb.2017.03.013

Abstract：In this study，the pigment gene in the red brown rice was mapped using F4 population by crossing of Shuhui 498（male） and Guihong 1（female）After the classical genetics analysis，it was confirmed that the red brown rice pigment of this experiment material was controlled by a pair of main genes，while other microgenes played a role of modification effectTwelve pairs of SSR primers with polymorphism were creened by gene pool method andUsing BAS method，12 pairs of polymorphism markers were screened between the parents and the gene pool；and they were respectively located on the 2nd，4th，5th，6th，7th and 10th chromosomesUsing mapmaker 30 location software，two quantitative trait locus（QTL） locations were identified：qAN10a located between RM258 and RM228 and qAN4a located between RM5414 and RM252qAN-10a had been detected on two sites of Jinping County and Huishu County，respectivelyqAN10a has good genetic stability and big gentic contribution rate，and was considered as the main effect gene of QTL，thus，provides a references to subsequent mapping of this major QTL

Key words：red brown rice；pigment gene；QTL

水稻為我國(guó)的傳統(tǒng)主食。世界稻種資源中有色稻種主要分布于中國(guó)、馬來(lái)西亞、印度尼西亞、緬甸、菲律賓國(guó)家和地區(qū)。目前中國(guó)保存的水稻種質(zhì)資源中，有色稻種占10% 左右，其中紅米稻種質(zhì)有8963份[1]，占據(jù)有色稻種的首位。

目前為止已經(jīng)完成了多個(gè)花色苷相關(guān)基因的定位。石幫志、王麗華、趙輝等[2-4]認(rèn)為紅米性狀是受一對(duì)主基因控制的顯性遺傳，位于第7號(hào)染色體上。廖金花[5]認(rèn)為紅米性狀是受一對(duì)主基因控制的顯性遺傳，其中第1染色體上的RM212與目的基因緊密連鎖。Nagao等[6]通過(guò)研究水稻自然突變體，發(fā)現(xiàn)紅米色素是由2對(duì)互補(bǔ)基因Rc和Rd控制。當(dāng)Rc和Rd同時(shí)存在時(shí)，種皮呈現(xiàn)暗紅色，當(dāng)Rc單獨(dú)存在時(shí)呈現(xiàn)褐色并有不規(guī)則的斑點(diǎn)，Rd單獨(dú)存在時(shí)種皮為白色。Sweeney等[7]利用red pericarp的普通野生稻與白種皮亞洲栽培稻品種構(gòu)建群體，將Rc定位于水稻第7號(hào)染色體上RM21197與RID15間的185kb的區(qū)段內(nèi)。……