SSR分子標記技術在漢青一號蘿卜種子純度鑒定中的應用
2016-12-31 00:00:00張小康朱運峰陳玉霞徐長城張雪麗熊秋芳
湖北農業科學 2016年19期
摘要：選用120對引物在漢青一號蘿卜（Raphanus sativus L. cv. HanQing NO.1）親本間進行PCR擴增，從中篩選出3對引物10、30、63，在雙親本間能夠擴增出差異互補的條帶，利用3對引物對漢青一號蘿卜雜種1代群體進行純度鑒定。鑒定結果純度為95.1%，與同批次種子的田間鑒定結果96.0%相近，說明應用SSR分子標記技術實施蘿卜雜種1代種子的純度鑒定是可行并可靠的。
關鍵詞：漢青一號蘿卜（Raphanus sativus L. cv. HanQing NO.1）；SSR分子標記技術；種子純度；鑒定
中圖分類號：S338；S631.1 文獻標識碼：A 文章編號：0439-8114（2016）19-5069-03
DOI：10.14088/j.cnki.issn0439-8114.2016.19.043
Abstract： 120 pairs of SSR primers were selected for PCR amplification between the parental lines of radish hybrid（Raphanus sativus L.） HanQing-No.1. In which 3 pairs of primers，10，30 and 63 could amplify difference-complementary bands from either of the parents. Using the 3 pairs of primers to identify the purity of HanQing-No.1， the results showed that the seed purity was 95.1%，which was approximate to the field purity（96% in the same batch of seeds identification）. The conclusion suggested that the application of SSR molecular markers technique could be feasible and reliable for the purity identification of radish hybrid seed.
Key words： Raphanus sativus L.； SSR molecular marker technique； seed purity； identification
