茶色素對(duì)糖尿病并發(fā)癥的防治

茶色素對(duì)糖尿病并發(fā)癥的防治

陳云

(河北鋼鐵集團(tuán)宣鋼職工醫(yī)院，河北 張家口 075100)

摘要：目的探討茶色素防治糖尿病(DM)并發(fā)癥的作用及機(jī)制。方法對(duì)85例口服茶色素膠丸的DM患者頭發(fā)進(jìn)行微量元素、血β2-微球蛋白(β2-MG)、血流變學(xué)與甲襞微循環(huán)檢測(cè)，觀察其治療前后各項(xiàng)指標(biāo)變化。結(jié)果服用茶色素膠丸后患者頭發(fā)鐵、錳含量顯著增高(P<0.01)；發(fā)鋅、銅含量呈現(xiàn)增加趨勢(shì)，但治療前后比較無(wú)統(tǒng)計(jì)學(xué)意義(P>0.05)；治療前后DM患者發(fā)鎘含量比較無(wú)統(tǒng)計(jì)學(xué)意義(P>0.05)；DM患者治療后β2-微球蛋白較治療前顯著降低(P<0.01)；血流變學(xué)檢查DM患者紅細(xì)胞沉降率、全血黏度比、全血還原黏度、血沉方程K、纖維蛋白原較治療前顯著下降(P<0.05)；DM患者服藥后甲襞微循環(huán)顯著改善(P<0.05)。結(jié)論茶色素對(duì)DM患者的“三微”有顯著的調(diào)節(jié)和改善作用，可防止DM多種慢性并發(fā)癥的發(fā)生。

關(guān)鍵詞：茶色素；糖尿病；微量元素；微量蛋白；微循環(huán)

DOI:10.13463/j.cnki.cczyy.2015.06.039

中圖分類(lèi)號(hào)：R255.4文獻(xiàn)標(biāo)志碼：A

文章編號(hào)：2095-6258(2015)06-1206-02

基金項(xiàng)目：河北省衛(wèi)生廳醫(yī)學(xué)科學(xué)研究課題(20130364)。

作者簡(jiǎn)介：陳云(1975-)，大學(xué)本科，主管藥師，主要從事藥學(xué)相關(guān)疾病研究。

收稿日期:(2015-08-18)

Prevention mechanism of tea pigment to diabetic complications

CHEN Yun

(Xuangang Worker’s Hospital of Iron and Steel Group of Hebei Province, Zhangjiakou 075100, Hebei Province, China)

Abstract：ObjectiveTo explore the effect of tea pigment in preventing the complications of diabetes mellitus (DM) and its mechanism. MethodsA total of 85 DM patients who were orally administered with tea pigment pills were included in the study. The microelement, β2-MG, hemorheology, and nail fold microcirculation were detected, and the changes of all various indicators before and after treatment were observed. ResultsAfter taking tea pigment pills, the contents of iron and manganese in the hair were significantly increased (P<0.01). The contents of zinc and copper after treatment showed an increased tend, but were not significantly different from those before treatment (P>0.05). The comparison of cadmium before and after treatment was not statistically significant (P>0.05). The content of β2-MG after treatment in DN patients was significantly reduced when compared with before treatment (P<0.01). The hemorheology examination results showed that ESR, whole blood viscosity ratio, whole blood reduced viscosity, blood sedimentation equation K, and fibrinogen after treatment in DM patients were significantly reduced when compared with before treatment (P<0.05). The nail fold microcirculation after treatment was significantly improved (P<0.05). Conclusion Tea pigment can significantly regulate and improve “three micro”, and plays a vital role in preventing various chronic complications of DM.

Keywords：tea pigment; DM; microelement; microprotein; microcirculati

研究[1-2]表明，茶色素對(duì)糖尿病(DM)并發(fā)癥防治具有顯著效果。筆者為探討茶色素防治DM并發(fā)癥的作用及機(jī)制，對(duì)85例服用茶色素膠丸治療的DM患者用藥前后頭發(fā)微量元素、β2-MG、甲襞微循環(huán)等進(jìn)行檢測(cè)，旨在探討其作用機(jī)制。報(bào)告如下。

1資料與方法

1.1一般資料選取我院內(nèi)分泌科就診的DM 患者85例，男40例，女45例，年齡36～68歲，平均(53.4±3.4)歲，病程2～25年。均符合世界衛(wèi)生組織(WHO)制定的DM診斷標(biāo)準(zhǔn)[3]。1.2治療方法控制血糖，并給予擴(kuò)血管、活血化瘀藥物及茶色素口服治療。茶色素膠丸(江西綠色制藥集團(tuán)公司生產(chǎn)，國(guó)藥準(zhǔn)字Z36021248)，125 mg/次，3次/d，服藥70 d為1療程。1.3檢測(cè)項(xiàng)目收集用藥前及治療1療程后進(jìn)行頭發(fā)微量元素Zn、Cu、Fe、Mn、Cd、血β2-MG、微循環(huán)及血流變等指標(biāo)進(jìn)行檢測(cè)。樣本采集：采集受試者枕部頭發(fā)，去梢，取根部2～3 cm，標(biāo)本重約0.5 g，濕發(fā)處理，進(jìn)行火焰測(cè)定；微球蛋白與血液流變學(xué)分別檢測(cè)用藥前后空腹血，同時(shí)觀察甲襞微循環(huán)。1.4統(tǒng)計(jì)學(xué)處理采用 SPSS 14.0 軟件對(duì)研究數(shù)據(jù)進(jìn)行分析，計(jì)量資料以均數(shù)±標(biāo)準(zhǔn)差(±s)表示，行t檢驗(yàn)，P<0.05為有統(tǒng)計(jì)學(xué)意義。

2結(jié)果

2.1服藥前后頭發(fā)微量元素變化比較見(jiàn)表1。

表1　茶色素對(duì)微量元素的影響( ± s, n=85)　mmol/g

表1　茶色素對(duì)微量元素的影響( ± s, n=85)　mmol/g

項(xiàng) 目鋅(Zn)銅(Cu)鐵(Fe)錳(Mn)鎘(Cd)治療前2.33±0.190.17±0.060.41±0.27 0.02±0.01 0.02±0.001治療后2.40±1.070.18±0.070.61±0.27#0.03±0.01#0.02±0.001

注：與治療前比較,#P<0.05

2.2服藥前后血β2-MG水平變化比較本組DM患者中有31例血β2-MG高于正常，其中男17例，女14例，服藥后血β2-MG水平較服藥前顯著下降，服藥前后比較差異有統(tǒng)計(jì)學(xué)意義(P<0.01)。

2.3服藥前后血黏度變化比較見(jiàn)表2。

表2　茶色素對(duì)血黏度的影響( ± s, n=85)

表2　茶色素對(duì)血黏度的影響( ± s, n=85)

項(xiàng) 目用藥前用藥后紅細(xì)胞壓積0.47±0.890.46±0.92 血沉/mm/h15.60±3.0011.00±3.01#全血黏度比(高切)8.54±0.416.87±0.52#全血黏度比(低切)15.83±7.2112.30±3.56#血漿黏度比1.85±0.221.72±0.25 全血還原黏度12.80±3.1011.50±2.00#血沉方程K130.02±58.2168.11±39.12#纖維蛋白原/(g/L)3.42±1.102.18±0.83#

注：與治療前比較,#P<0.05

2.4服藥前后甲襞微循環(huán)的變化見(jiàn)表3。

表3　茶色素對(duì)甲襞微循環(huán)的變化

注：與治療前比較，#P<0.05

3小結(jié)

本研究結(jié)果顯示，茶色素可參與細(xì)胞代謝，延緩細(xì)胞的衰老，減少β2-MG生成，從而增強(qiáng)機(jī)體免疫功能[4-6]。茶色素能有效消除自由基，降低血漿脂質(zhì)過(guò)氧化物的含量，減少超氧化物歧化酶的消耗，從而改善腎功能，茶色素能明顯降低纖維蛋白原、全血黏度、紅細(xì)胞沉降率，從而起到抗凝，促纖溶作用，改善DM患者微循環(huán)瘀滯狀態(tài)，防治DM及其并發(fā)癥[7-8]。

參考文獻(xiàn)：

[1]陳來(lái)蔭,陳榮山,葉陳英,等.茶色素的提取,功效及應(yīng)用研究進(jìn)展[J].茶葉通訊,2013,40(2):32-35.

[2]SIL H, SEN T, MOULIK S, et al. Black tea polyphenol (theaflavin) downregulates MMP-2 in human melanoma cell line A375 by involving multiple regulatory molecules[J]. Journal of Environmental Pathology, Toxicology and Oncology : Official Organ of the International Society for Environmental Toxicology and Cancer, 2010, 29(1): 55-68.

[3]張片紅,何芳,陳瑩瑩,等.茶色素對(duì)腦缺血再灌注損傷大鼠一氧化氮合酶表達(dá)的影響[J].浙江預(yù)防醫(yī)學(xué),2011,23(8):14-15.

[4]楊新河,呂幫玉,毛清黎,等. 茶色素的生物活性研究進(jìn)展[J].江西農(nóng)業(yè)學(xué)報(bào),2012, 24(1):102-105.

[5]YOSHINO K, YAMAZAKI K, SANO M. Preventive effects of black tea theaflavins against mouse type IV allergy[J]. Journal of the Science of Food and Agriculture, 2010, 90(12): 1983-1987.

[6]LAHIRY L, SAHA B, CHAKRABORTY J, et al. Theaflavins target Fas/caspase-8 and Akt/pBad pathways to induce apoptosis in p53-mutated human breast cancer cells[J]. Carcinogenesis, 2010, 31(2): 259-268.

[7]陳麗如,張娜,楊更亮,等.茶色素的超聲輔助提取及其穩(wěn)定性研究[J].江西農(nóng)業(yè)大學(xué)學(xué)報(bào),2010,32(3):608-612.

[8]金恩惠,吳媛媛,屠幼英.茶黃素抑菌作用的研究[J].中國(guó)食品學(xué)報(bào),2011,11(6):108-112.