依達拉奉對肺泡Ⅱ型上皮細胞NF—κB和IL—6，IL—8表達及凋亡的影響

王媛媛+曹建+陳勤+等

1.南昌市第三醫院心內科，江西南昌 330009；2.南昌大學第二附屬醫院麻醉科，江西南昌 330006

[摘要] 目的 探討依達拉奉對肺泡Ⅱ型上皮細胞凋亡的影響及NF-κB和白介素-6（IL-6）、白介素-8（IL-8）表達。 方法 體外培養肺泡Ⅱ型上皮細胞，用過氧化氫氧化應激建立肺泡Ⅱ型上皮細胞凋亡細胞模型，將細胞分為對照組、過氧化氫組、依達拉奉-過氧化氫組。用MTT法檢測依達拉奉對肺泡Ⅱ型上皮細胞增殖能力的影響，流式細胞儀檢測依達拉奉對氧化應激肺泡Ⅱ型上皮細胞細胞凋亡的影響，采用ELISA試劑盒及谷胱甘肽（GSH）、脂質過氧化物（MDA）試劑盒檢測IL-6、IL-8及GSH、MDA含量，用Western-blot方法檢測氧化應激肺泡Ⅱ型上皮細胞中的NF-κB蛋白表達。 結果 依達拉奉-過氧化氫組細胞生長抑制率為（22.3±3.1）%，明顯低于過氧化氫組[（37.5±2.4）%]，差異有統計學意義（P < 0.05）。依達拉奉-過氧化氫組MDA含量低于過氧化氫組，GSH含量高于過氧化氫組，差異均有統計學意義（P < 0.05）。依達拉奉-過氧化氫組IL-6、IL-8含量低于過氧化氫組，差異均有統計學意義（P < 0.05）。依達拉奉-過氧化氫組細胞凋亡率[（14.67±0.50）%]明顯低于過氧化氫組[（38.88±0.80）%]，差異有統計學意義（P < 0.05）。過氧化氫+依達拉奉組NF-κB蛋白水平（2.106±0.012）低于過氧化氫組（4.216±0.127），差異有統計學意義（P < 0.05）。 結論 依達拉奉具有抑制氧化應激后所誘導肺泡Ⅱ型上皮細胞凋亡、下調NF-kB和IL-6、IL-8表達的作用。

[關鍵詞] 依達拉奉；氧化應激；核因子-κB；白介素-6；白介素-8；肺泡Ⅱ型上皮細胞；凋亡

[中圖分類號] R563 [文獻標識碼] A [文章編號] 1673-7210（2014）03（a）-0008-05

Effects of Edaravone on apoptosis and the expressions of NF-κB， IL-6， IL-8 in lung epithelial type Ⅱ cells

WANG Yuanyuan1 CAO Jian2 CHEN Qin1 OUYANG Xianguo1 SONG Guoliang1

1.Department of Cardiology， the Third Hospital of Nanchang City， Jiangxi Province， Nanchang 330009， China； 2.Department of Anesthesiology， the Second Affiliated Hospital of Nanchang University， Jiangxi Province， Nanchang 330006， China

[Abstract] Objective To explore the effects of Edaravone on apoptosis and the expressions of NF-κB， IL-6， IL-8 in lung epithelial type Ⅱ cells. Methods Lung epithelial type Ⅱ cells were cultured in vitro， the apoptosis of lung epithelial type Ⅱ cells model was established by hydrogen peroxid. Lung epithelial type Ⅱ cells were divided into three groups： control group， hydrogen peroxide group and edaravone-hydrogen peroxide group， lung epithelial type Ⅱ cells was treated by edaravone. The proliferation rate of lung epithelial type Ⅱ cells were detected by MTT； the apoptosis rate of lung epithelial type Ⅱ cells was determined though flow cytometry； the expression of IL-6， IL-8， GSH， MDA was detected by ELISA kit， GSH kit， MDA kit， the NF-κB protein expression after edaravone treatment was examined through Western-blot. Results Cell growth inhibition rate in edaravone-hydrogen peroxide group [（22.3±3.1） %] was lower than that in hydrogen peroxide group [（37.5±2.4） %]， the difference was statistically significant （P < 0.05）. MDA content in edaravone-hydrogen peroxide group was lower than that in hydrogen peroxide group， GSH content in edaravone-hydrogen peroxide group was higher than that in hydrogen peroxide group， the differences were statistically significant （P < 0.05）. IL-6， IL-8 content in edaravone-hydrogen peroxide group was lower than that in hydrogen peroxide group， the difference was statistically significant （P < 0.05）. Cell apoptosis rate in edaravone-hydrogen peroxide group [（14.67±0.50） %] was lower than that in hydrogen peroxide group [（38.88±0.80） %]， the difference was statistically significant （P < 0.05）. NF-κB protein level in edaravone-hydrogen peroxide group （2.106±0.012） was lower than that in hydrogen peroxide group （4.216±0.127）， the difference was statistically significant （P < 0.05）. Conclusion Edaravone can inhibit lung epithelial type Ⅱ cells apoptosis against -oxidative stress and down-regulate the expressions of NF-κB and IL-6， IL-8.

[Key words] Edaravone； Oxidative stress； NF-κB； IL-6； IL-8； Lung epithelial type Ⅱ cells； Apoptosis

急性肺損傷發病過程中，細胞因子可能通過抑制炎癥細胞凋亡延長炎性反應時間，促進肺泡上皮細胞凋亡而參與急性肺損傷的炎性反應過程[1]。……