中藥及復方水提物對DPPH·和·OH的清除作用

中藥及復方水提物對DPPH·和·OH的清除作用

龔永歡，劉 霞，周海霞，李 偉，馬 林

（西南科技大學生命科學與工程學院，四川 綿陽 621000）

摘要：采用分光光度法分析測定了具有抗仔豬腹瀉作用的12種中藥及5個復方的水提物對1，1-二苯基-2-苦基肼自由基（DPPH·）和羥基自由基（·OH）的清除能力，并對其多糖含量進行了測定。結果表明，12種中藥及5個復方對DPPH·和·OH均有一定程度的清除作用。濃度在0.2～1.0 mg/mL范圍內，秦皮、白頭翁、黃連、黃柏以及復方2、4、5對DPPH·清除作用較好，在高濃度時與維生素C對DPPH·的清除作用相當。除復方3外，中藥復方在高濃度時對DPPH·的清除率接近或高于其組成中藥，表現出協同效應。濃度在5～25 mg/mL范圍內，白術、防風、魚腥草及復方1和復方3對·OH的清除作用較好，復方1和復方3表現出對·OH清除的協同作用。中藥及其復方多糖含量與DPPH·和·OH清除作用不存在明顯的相關性。

關鍵詞： 抗腹瀉中藥；抗氧化；1，1-二苯基-2-苦基肼自由基；羥基自由基

中圖分類號：R932 文獻標識碼：A 文章編號：0439-8114（2013）20-4995-05

Scavenging Effect of Aqueous Extracts from Some Anti-diarrhea Chinese Herbs and Compounds on DPPH· and ·OH

GONG Yong-huan，LIU Xia，ZHOU Hai-xia，LI Wei，MA Lin

（School of Life Science and Engineering， Southwest University of Science and Technology， Mianyang 621000， Sichuan， China）

Abstract： Using spectrophotometric and phenol-sulfuric acid method， the scavenging effect on DPPH and hydroxyl radicals， and the polysaccharide contents in the aqueous extracts from 12 Chinese herbs and 5 compounds with anti-diarrhea effects were investigated. The results showed that all 12 Chinese herbs and 5 compounds had scavenging effect on DPPH· and ·OH with different degree. With the concentration of 0.2 to 1.0 mg/mL， the Cortex fraxini， Radix pulsatillae， Rhizoma coptidis and Cortex phellodendri and the compounds 2， 4 and 5 had high scavenging effects on DPPH·. The scavenging effect was close to that of vitamin C at 0.8 mg/mL and 1.0 mg/mL. Except the compounds 3， other compounds had synergistic effect and showed higher scavenging effect on DPPH than that of its component herbs with tested concentration. Under the concentration of 5 mg/mL to 25 mg/mL， the extracts from Rhizoma atractylodis， Radix saposhnikoviae and Houttuynia cordata and compounds 1 and 3 had good scavenging effect on hydroxyl radicals. Similarly， the compounds 1 and 3 showed synergistic effect. There was no significant correlation between the polysaccharide content of different herbs， compounds and the scavenging effect on DPPH· and ·OH.

Key words： anti-diarrhea Chinese herbs； antioxidant； DPPH·； ·OH

早期斷奶仔豬因消化器官發育不充分、消化酶分泌不足、免疫力低下等極易帶來嚴重的應激反應，導致腸道系統紊亂及腹瀉[1]。正常情況下，機體內自由基的產生與清除處于動態平衡，維持著機體的正常生理機能，但當仔豬在遭受患病、應激或者日糧中營養缺乏等情況下，機體代謝出現異常，自由基將過量產生。自由基活性極高，攻擊性很強，導致機體發生一系列變化，致使動物病理狀況加重[2，3]。中國全年仔豬平均腹瀉發病率為46.5%[4]，因腹瀉死亡的仔豬占仔豬總數的39.8%[5]，給養豬業帶來巨大的經濟損失。……